...CLC204 – Analytic Chemistry Experiment Quantitative Analysis of an Unknown Liquid Sample Objective: To be familiar with Ultra Violet Spectrophotometer (UV) and Gas Chromatography (GC) used in chemical analysis UV Abstract In this report, people who consumed tom yum soup suffered from nausea and vomiting due to copper poisoning which was found to leach from a pot into the soup under high heat and acidic condition. It was also suspected that an organic compound was present in the soup which enhanced the absorption of copper in consumer. Hence, as an analytical chemist, we have to use UV to determine the actual concentration of copper standards and blank using external calibration standards. The result of the test solution was measured by comparing it with the calibration of copper. Introduction The goal of this experiment is to obtain the concentration of copper in a known solution. Therefore, in this experiment, we will be using UV to measure the absorbance of the solution. A spectrophotometer is used to measure the amount of light that a sample absorbs. Ultraviolet (UV) light is an electromagnetic radiation with a wavelength in the range of 10nm to 400nm and energy from 3eV to 124Ev. With a shorter wavelength as compared to visible light, UV light is able to penetrate more readily through obstacles. Its name came from a spectrum which humans identify as the colour violet. UV light is invisible, but it can be seen indirectly when it makes other substances...
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...in a person’s body has the same DNA. Ribonucleic acid or RNA, is one of the three major macromolecules (along with DNA and proteins) that are essential for all known forms of life. A pipette (also called a pipet, pipettor or chemical dropper) is a laboratory instrument used to transport a measured volume of liquid. Pipettes come in several designs for various purposes with differing levels of accuracy and precision, from single piece glass pipettes to more complex adjustable or electronic pipettes. Lab dish washing Cleaning laboratory glassware isn't as simple as washing the dishes. Here's how to wash your glassware so that you won't ruin your chemical solution or laboratory experiment. You can rinse the glassware with the proper solvent, then finish up with a couple of rinses with distilled water, followed by final rinses with deionized water Water Soluble Solutions (e.g., sodium chloride or sucrose solutions) Rinse 3-4 times with deionized water then put the glassware away. Water Insoluble Solutions (e.g., solutions in hexane or chloroform) Rinse 2-3 times with ethanol or acetone, rinse 3-4 times with deionized water, then put the glassware away. In some situations other solvents need to be used for the initial rinse. Lab chemical stock maintenance • Remove items from the written inventory as they are disposed or used • Record all new orders • Record the received date on...
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...parts. In the first part, you will analyze an unknown solution for the presence of seven common ions. In the second part, you will test an unknown solid to determine which of two possible identities is correct. Part I. Spot Tests for Some Common Ions A simple approach to the qualitative analysis of an unknown solution is to test for the presence of each possible ion by adding a reagent which will cause the ion, if it is in the sample, to react in a characteristic way. This method involves a series of “spot” tests, one for each ion, carried out on separate samples of the unknown solution. The difficulty with this way of doing qualitative analysis is that frequently, particularly in complex mixtures, one species may interfere with the analytical test for another. Although interferences are common, there are many ions which can be identified in mixtures by simple spot tests. In this experiment we will use spot tests for the analysis of a mixture which may contain the following commonly encountered ions in solution: CO32– SO42– PO43– SCN Cl – – carbonate sulfate phosphate thiocyanate chloride acetate ammonium C2H3O2– NH4+ 78 Chemistry 1B Experiment 16 The procedures we involve simple acid-base, precipitation, complex ion formation or oxidation-reduction reactions. You will carry out each test three times. First, you will test 1 M solutions of each ion, so you can easily observe the expected results. Then you will test 0.1 M solutions of each ion, which will require...
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...Clean Water Project Report Day 1- “The Balanced Equation” Documentary Notes In the documentary, “The Balanced Equation”, a group of high school girls tells their story of traveling to Kenya and the Dominican Republic to investigate and learn more about issues dealing with water in developing countries. The girls focus primarily on problems with water that exist in these countries and the water filtration systems being provided. According to the video, approximately 3.5 million people die from water-related illnesses each year, and over 800 million people do not have access to clean water today. The girls discover that portable point-of-use filtration devices are given to those in developing countries who don’t have access to clean water. Permanent water solutions, such as digging wells, have been tried, but often fail due to lack of maintenance and education. Wells often become contaminated with runoff water among other issues, so over 50% of these permanent solutions fail after 5 years. Instead, point-of-use water systems are distributed since they are portable and can be used at home. The girl’s spent their first night of their adventure in...
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... They lack a distinct nucleus and occur singly or in chains or clusters and form distinct colonies. Bacteria are classified on the basis of many characteristics. Morphological and physiological features such as cell shape, motility, formation of spores and other distinguishable structures, and reaction to Gram stain is a good start in identifying bacteria. Other staining techniques such as Acid Fast stain are also useful in determining species. More important in identification of a genus and species of bacteria are biological tests, including the determination of the types of nutrients a cell can use, the products of its metabolism, and the response to specific chemicals. Other factors that can assist in identification of bacteria are their ecological habitats and more advanced methods such as genetic and molecular composition. Using various techniques one is able to distinguish and ultimately assign then genus and species of the unknown bacteria. Methods: Gram Staining: The Gram stain separates bacteria in two distinct classes and is also useful in distinguishing morphology. Through this technique one is able to identify bacteria as either gram positive or gram negative. The gram-negative bacteria have thin peptidoglycan layers that do not hold the primary stain, rather...
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...an explosion in both the use of traditional materials and in the research for development of a wider range of materials to satisfy technological developments. Added to this was a reduction in availability of the traditional resources to supply the increasing world population. Chemists and chemical engineers continue to play a pivotal role in the search for new sources of traditional materials such as those from the petrochemical industry. As the fossil organic reserves dwindle, new sources of the organic chemicals presently used have to be found. In addition, chemists are continually searching for compounds to be used in the design and production of new materials to replace those that have been deemed no longer satisfactory for needs. This module increases students’ understanding of the implications of chemistry for society and the environment and the current issues, research and developments in chemistry. 1.1 Construct word and balanced formulae equations of all chemical reactions as they are encountered in this module: • Acid reactions: o acid (aq) + base (aq) salt (aq) + water (l) o acid (aq) + active metal (s) salt (aq) + hydrogen (g) o acid (aq) + metal carbonate (s) salt (aq) + water (l) + carbon dioxide (g) • Cracking of long chain alkanes (e.g. decane) to give a shorter chain alkene & alkane o Thermal cracking (heated with steam in absence of O2 to 750-900°C) Initiation: C10H22 2C5H11· Propagation: C5H11· C3H7· + C2H4 Termination: 2C3H7· C6H14 o Catalytic cracking ...
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...of chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition. Typical uses of GC include testing the purity of a particular substance, or separating the different components of a mixture (the relative amounts of such components can also be determined). In some situations, GC may help in identifying a compound. In preparative chromatography, GC can be used to prepare pure compounds from a mixture.[1][2] In gas chromatography, the mobile phase (or "moving phase") is a carrier gas, usually an inert gas such as helium or an unreactive gas such as nitrogen. The stationary phase is a microscopic layer of liquid or polymer on an inert solidsupport, inside a piece of glass or metal tubing called a column (a homage to the fractionating column used in distillation). The instrument used to perform gas chromatography is called a gas chromatograph (or "aerograph", "gas separator"). The gaseous compounds being analyzed interact with the walls of the column, which is coated with a stationary phase. This causes each compound to elute at a different time, known as the retention time of the compound. The comparison of retention times is what gives GC its analytical usefulness. Gas chromatography is in principle similar to column chromatography (as well as other forms of chromatography, such as HPLC, TLC), but has several notable differences. First, the process of separating the compounds in a mixture is carried out between a liquid stationary...
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...Classification Tests for Hydroxyl- and Carbonyl-containing Compounds Miguel, K.D., Moron, R.S.S., Pazon, A.D., Ramirez, C.V., Raquepo, T.M.R., Razon, D.N.A.Jr. 2B-PH, Group No. 6, Department of Pharmacy, Faculty of Pharmacy, University of Santo Tomas, España Boulevard, 1015 Manila, Philippines Abstract In organic chemistry it is very common to see molecules comprised mainly of a carbon backbone with functional groups attached to the chain. The functional group gives the molecule its properties, regardless of what molecule contains it.[1] Examples of functional groups are that of hydroxyl (-OH) which is usually seen in alcohols, and carbonyl (C=0) which is seen in aldehydes and ketones. In this experiment, several tests were conducted to distinguish and differentiate various sample compounds such as ethanol, n-butyl alcohol, sec-butyl alcohol, tert-butyl alcohol, benzyl alcohol, n-butyraldehyde, benzaldehyde, acetone, acetophenone, isopropyl alcohol, and acetaldehyde. The tests are the following: solubility test of alcohols in water, which gave a soluble result in ethanol, sec-butyl alcohol and tert-butyl alcohol. Next is the Lucas test, which is used to differentiate 1°, 2° & 3° alcohols. In Lucas test, tert-butyl alcohol gave an immediate turbid result; the rate of reaction was noted. Chromic Acid test (Jones Oxidation) which gave a positive result by producing a blue-green solution with the sample n-butyl alcohol, acetaldehyde, benzaldehyde, and isopropyl alcohol. 2...
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...Preparation of standard solution and standardization of hydrochloric acid To prepare a standard solution of sodium carbonate and use it to standardize a given solution of dilute hydrochloric acid. Anhydrous sodium carbonate is a suitable chemical for preparing a standard solution (as a primary standard). The molarity of the given hydrochloric acid can be found by titrating it against the standard sodium carbonate solution prepared. The equation for the complete neutralization of sodium carbonate with dilute hydrochloric acid is Na2CO3(aq) + 2HCl(aq) → 2NaCl(aq) + CO2(g) + H2O(l) The end-point is marked by using methyl orange as indicator. Introduction : Chemicals : Apparatus : Procedure : solid sodium carbonate, 0.1 M hydrochloric acid 1. 2. 3. 4. 5. 6. 7. 8. Weight out about 1.3 g of anhydrous sodium carbonate accurately using the method of “weighing by difference”. Transfer the weighed carbonate to a beaker and add about 100 cm3 of distilled water to dissolve it completely. After dissolving, transfer the solution to a 250.00 cm3 volumetric flask. Rinse the beaker thoroughly and transfer all the washes into the volumetric flask. Remember not to overshoot the graduation mark of the flask. Make up the solution to the mark on the neck by adding water. Pipette 25.00 cm3 of sodium carbonate solution to a clean conical flask. Add 2 drops of methyl orange indicator to the carbonate solution. Titrate the carbonate solution with the given dilute hydrochloric acid until the colour...
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...Percent Copper in a Penny Lab Report Chemistry 101 Section #02 Washington State University By Sadie Yost April 15, 2024 Partner: Abigail Swenson. Introduction This lab was conducted to help students learn how to develop their own experiment by using knowledge gained from previous labs to determine the amount of copper in a 1983 or newer penny. Information from previous labs was used to help students properly collect and analyze data. Throughout the experiment, notetaking and observational skills were also tested. The U.S. Mint was first opened in 1793, and pennies were originally crafted out of 100% pure copper. By 1857, the composition of the penny was altered to incorporate nickel and then switched to a combination of tin and zinc in...
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...Chapter 1 The Problem and Its Setting Introduction Thrombocytopenia or abnormally low platelet counts in the body are typically caused by genetic disorder or fatal diseases. This condition may result from thromboembolic or hemorrhagic complications caused by heparin as well as from various diseases states such as chronic liver disease and dengue hemorrhagic fever. In most rural areas a health care institution is often inaccessible and most patients cannot afford high cost medication. Henceforth, herbal medicines are often used as an alternative treatment. Inadequacy of providing supportive treatment to correct the decrease of platelet is fatal to patients. Thus, several decoctions of herbal plants are purportedly used to improve platelet count. Recently, the mortality rate in heparin – induced thrombocytopenia (HIT) has been reported as high as 20% (Greinacher & Lubanow, 2003). Retrospective data shows that up to 8% of heparinized patients will develop the antibody associated with HIT and that approximately 1–5% of patients on heparin will progress to develop HIT with thrombocytopenia suffering from venous and/or arterial thrombosis in at least one-third of cases (Warkentin et al., 1998). Heparin – induced thrombocytopenia (HIT) is a potentially devastating immune mediated adverse drug reaction caused by the emergence of antibodies that activate platelets in the presence of heparin. Despite thrombocytopenia, bleeding is rare; rather, HIT is strongly associated with...
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...EXPERIMENT 1 DETERMINATION OF DISSOLVED OXYGEN IN WATER INTRODUCTION The dissolved oxygen content is an important index when considering its suitability for town supply. A good clean potable water will give dissolved oxygen value close to the theoretical value for the saturated solution of oxygen in water. When there is pollution from organic matter and other trade effluents, the dissolved oxygen is up in various biochemical oxidation processes and its is only slowly replaced through surface absorption. Such water will give a low dissolved oxygen content until oxidation is completed. Adequate dissolved oxygen is necessary for the life of fish and other aquatic organisms. The methods described below for the determination of oxygen in water is based on that devised by Winkler. When manganese hydroxide is precipitated in the water sample it is quickly oxidized to higher hydrated oxides (probably in the four valent state) by the dissolve oxygen. Iodine, equivalent to the dissolved oxygen content, is then liberated on acidification in the presence of iodine, and it may be titrated with standard thio‐sulphate. INTERFERENCES AND PRE – TREATMENT Most oxidising and reducing substances e.g dissolved organic substances, nitrite ions, higher‐valency manganese compounds, active chlorine, sulphide and sulphite ions, iron (II) and irons interfere. The influence of the dissolved organic substances can be excluded by conversion of the manganese hydroxides...
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...the requirements of the current arrangements. Acknowledgement © Crown copyright 2012. You may re-use this information (excluding logos) free of charge in any format or medium, under the terms of the Open Government Licence. To view this licence, visit http://www.nationalarchives.gov.uk/doc/open-government-licence/ or e-mail: psi@nationalarchives.gsi.gov.uk. Where we have identified any third party copyright information you will need to obtain permission from the copyright holders concerned. Any enquiries regarding this document/publication should be sent to us at enquiries@educationscotland.gov.uk. This document is also available from our website at www.educationscotland.gov.uk. Contents Introduction 5 Chemical analysis 6 Qualitative and quantitative analysis 6 Volumetric analysis 6 Gravimetric analysis 14 Colorimetric analysis 17 Organic techniques 22 Introduction 22 Preparation 22 Isolation 24 Purification 29 Identification 33 Percentage yield 37 Errors 39 Accuracy and precision 39 Repeatability and reproducibility 41 Quantifying errors 41 Absolute uncertainties and percentage uncertainties 42 Combining uncertainties 43 Some ‘forgotten’ uncertainties 46 Experiments 53 Experiment 1A: Preparation of a standard solution of 0.1 mol l–1 oxalic acid 53 Experiment 1B: Standardisation of approximately 0.1 mol l–1 sodium hydroxide 55 Experiment 1C: Determination of the ethanoic acid...
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...detection times . . . . . . . . . . . . . . . 2.4.1 Physical Activity . . . . . . . . . . . . . . . . 2.4.2 Diet . . . . . . . . . . . . . . . . . . . . . . . 2.4.3 Using Drugs to Reduce Detection Times . . . 3 Test Methods 3.1 Substances that are Detectable . . . . . . . . . . . 3.2 DrugAlert . . . . . . . . . . . . . . . . . . . . . . . 3.3 Gas Chromatography . . . . . . . . . . . . . . . . . 3.4 Gas Chromatography / Mass Spectrometry . . . . . 3.5 Hair testing . . . . . . . . . . . . . . . . . . . . . . 3.6 High Performance Liquid Chromatography . . . . . 3.7 ImmunoAssay . . . . . . . . . . . . . . . . . . . . . 3.7.1 Radio ImmunoAssay (aka AbuScreen) . . . 3.7.2 Enzyme Multiplied Immunoassay Technique 3.7.3 Fluorescence Polarization ImmunoAssay . . 3.8 PharmChek . . . . . . . . . . . . . . . . . . . . . . 3.9 TestCup . . . . . . . . . . . . . . . . . . . . . . . . 3 11 13 13 13 15 16 16 16 17 17 18 18 18 19 19 21 21 21 22 24 24 25 25 25 26 26 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 CONTENTS 3.10 Thin Layer Chromatography . . . . . . . . . . . . . . . . . . . 26 4 Test...
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...artery to the lungs and returns to the left atrium through the pulmonary veins. Blood then enters the left ventricle to be circulated again. (Fig 1: http://upload.wikimedia.org/wikipedia/commons/thumb/e/e5/Diagram_of_the_human_heart_%28cropped%29.svg/220px-Diagram_of_the_human_heart_%28cropped%29.svg.png) Blood is a type of evidence found most often at violent crime scenes and submitted to forensic laboratories for further identification and analysis. The investigators at the crime scene always seek to answer three questions when in contact with bloodstains: (1) Is this blood? (2) Is it from a human? (3) How closely does it match the blood of the victim or suspect? Answers to these questions can be found through several analyses and tests carried out by the forensic...
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