Determination of Acetic Acid in Vinegar Introduction: Vinegar is a dilute solution of acetic acid. Since vinegar is an acid it can be titrated with a base. Titration is the process of adding a known amount of a solution of known concentration to a known amount of solution of unknown concentration. The more accurately the concentration of the solution of known concentration is known, the more accurately the concentration of the unknown solution can be determined. Some chemicals can be purchased
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Experiments in Chemistry Lecture 38 : Titrations : Acid-Base, Redox and Complexometric Objectives In this lecture you will learn the techniques to do following Determination of the amount of sodium carbonate and sodium hydroxide in a mixture by titration. Carrying out acid-base titration using a pH meter. Carrying out acid-base titration by conductometric measurement. Determination of the composition of a mixture of acetic acid and hydrochloric acid by conductometric titration. Determination of
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Determination of Acetic Acid in Vinegar Introduction: Vinegar is a dilute solution of acetic acid. Since vinegar is an acid it can be titrated with a base. Titration is the process of adding a known amount of a solution of known concentration to a known amount of solution of unknown concentration. The more accurately the concentration of the solution of known concentration is known, the more accurately the concentration of the unknown solution can be determined. Some chemicals can be purchased
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review 7.1 a-acid b- acid c- acid d-base 7.3 a-hydrochloric acid b-calcium hydroxide c-carbonic acid d-nitric acid e-sulfurous acid 7.5 a- Mg(OH)2 b-HF c- H3PO4 d- LiOH e- Cu(OH)2 7.7 a- HI is the acid and F- is the base b- H20 is the acid and f- is the base 7.9 a- F- fluoride ion b-OH- hydroxide ion c- HCO3- bicarbonate ion d- So42- sulfate ion 7.11 a- HCCO3- bicarbonate ion b- H3O+ hydronium ion c- H3PO4 phosphoric acid d- HBr hydrobromic
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Title of Experiment 9: Measurement of Nucleic Acid Solutions Objectives: 1. To study the principle of gel electrophoresis. 2. To use gel electrophoresis method to measure the nucleic acid solutions. 3. To learn the technique of nuclei acid measurement by using gel electrophoresis. Introduction: Gel electrophoresis is a widely used technique for the analysis of nucleic acids and proteins. Most every molecular biology research laboratory routinely uses agarose gel electrophoresis for the
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LAW 1. Calculate the dissociation constant of monobasic acid which is 3.5% dissociated in N/20 solution at 20o C. (6.34x10-5) 2. Calculate the hydrogen ion concentration in 10 liters of 0.1 N solution of an acid having dissociation constant 4.0x10-10 . (6.32x10-6g ion liter-1 ) 3. Nicotinic acid (Ka =1.4x10-5) is represented by the formula HNic. Calculate
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in microbiological systems. The chemistry of the predominant biological elements of groups I to VII will be discussed. The students will explore the principles of inorganic chemical phenomenon including partially soluble substances, weak acids and bases, buffer systems and redox reactions. In addition, a basic understanding of the chemistry of the biologically important elements (H, C, N, O, K, Na, Ca, Mg, P, S, Cl and some transitional elements) will be explored. Course Learning
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Andrew NB 1110 / M 5 – 7:50 Lab 1 February 2, 2015 pH and Buffer – Using Red Cabbage Indicator to Measure pH Introduction This experiment was performed to evaluate the following hypothesis: Red cabbage indicator can be used as a measure of pH. According to Campbell, et al, in Biology: Concepts and Connections, red cabbage contains a water-soluble pigment called anthocyanin that changes color when it is mixed with an acid or a base. The pigment turns red in acidic environments with a pH less
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size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.[1] Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through an agarose matrix. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores
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Isolation of Onion DNA. Abstract Agarose Gel Electrophoresis method was applied on an onion sample to isolate onion DNA. The objective of this experiment was to be able to extract the DNA of an onion in the presence of Ethylenediaminetetraacetic acid. The A260A280 absorbance ratios of isolated DNA were around 1.0593, suggesting that the DNA was not pure and could not be used for further analysis. Moreover, the A260A280 values were lower than 1.8, suggesting that there was a contamination by proteins
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