graduated pipette 1 x 20 cm3 of stock NADH (80 g cm-3) 1 x 20 cm3 of 50 mM phosphate buffer pH 7.2 2 x semi micro cuvettes Distilled water wash bottle Access to a Whirly mixer Access to Novaspec digital spectrophotometer 1 x 5 cm3 of a solution of NADH of unknown concentration. Method: 1. Using the protocol depicted in Table 1 set up 6 standard concentrations of NADH in buffer ranging from 0 to 80 µg cm-3. Table 1: Protocol for NADH standard curve. Tube Number. | 1 | 2 | 3 |
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Buffer solutions and common ion effect A buffer solution resists (or buffers) a change in its pH. That is, we can add a small amount of an acid or base to a buffer solution and the pH will change very little. How to calculate pH of buffer solution containing both acid and conjugate base? Dissociation constant definition 1.1 can be rearranged into [pic] or [pic] (note that due to sign change [A-] was moved to nominator). This is so called Henderson-Hasselbalch equation (or buffer equation)
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the following mixture problem. Problem: What quantity (amount) of a 60% acid solution must be mixed with a 30% acid solution to produce 300 mL of a 50% acid solution? Analysis: In this analysis (an integral part of the solution process) I will state the steps in logical order. Beneath each step, in small blue indented type, I will state the reason/logic/explanation for that step. (1) Let x be the amount of the 60% solution to be added. Begin by assigning a variable to the quantity (not the rate) of
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Running head: PROBLEM SOLUTION: GLOBAL COMMUNICATIONS Problem Solution: Global Communications John Doe University of Phoenix Problem Solution: Global Communications Problem-based learning allows the student to develop his or her problem-solving skills by applying them to an authentic scenario that requires them to identify a problem, apply a problem-solving approach to develop and analyze alternative solutions, and recommend and defend an optimal solution (University of Phoenix, 2010
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Buffering Work and Family Conflict Cyndi Willis Author note: This assignment was made by Cyndi Willis on July 25, 2014 for Kathryn Chiplis’ Work and Family course at Rasmussen College. Buffering Work and Family Conflict There are many things that create conflict when one is trying to balance their work life and their family life. Sometimes it becomes increasingly overwhelming trying to keep a job and move up the ranks to a higher paying position while still pleasing your spouse, kids, and just
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Introduction Buffer is a solution that resists a change in pH when bases or acid are added. Solutions that are acidic contain high concentrations of hydrogen ions (H+) and have pH values less than seven. Buffer usually consist of a weak acid, and its conjugate base or a weak base and its conjugate acid. The function of buffer is to resist the changes in hydrogen ion concentration as a result of internal and environmental factor. This buffer experiment is important so that we relies the important
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|6 |150 | As the numbers of products are natural numbers, there are two more constraints. |X1 ≥ 0 | |X2 ≥ 0 | |4. Find the set of feasible solutions that graphically represent the constraints. | | | | | |
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THE PASSAGE OF SALYCILATE INTO YEAST CELLS AIM: Aim of the experiment is to investigate and analyse the effect of pH on the transfer of salicylate across membranes. INTRODUCTION: As Schedule METHOD: As Schedule RESULTS: Test Tube number | Absorbance | 1 | 0 | 2 | 0.171 | 3 | 0.337 | 4 | 0.496 | 5 | 0.598 | 6 | 0.801 | 7 | 1.598 | 8a | 0.46 | 9a | 0.629 | 10a | 0.701 | 11a | 0.899 | 8b | 0.704 | 9b | 0.793 | 10b | 0.830 | 11b | 0.877 | 1. 1.0 x (PCV/100)X0
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points of view, and life experiences as tools to produce a quality solution or product for the course. The variation of ideas can then allow each team member to explore ideas and approaches that they would otherwise not have thought of or considered. In essence, there is an exchange of knowledge that allows each member to complete the project with the realization that the variation of skills and knowledge has created a better solution. Each member has to assist in the shared responsibility of a group
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Running head: PROBLEM SOLUTION: GLOBAL COMMUNICATIONS Problem Solution: Global Communications John Smith University of Phoenix Problem Solution: Global Communications Problem-based learning allows the student to develop his or her problem-solving skills by applying them to an authentic scenario that requires them to identify a problem, apply a problem-solving approach to develop and analyze alternative solutions, and recommend and defend an optimal solution (University of Phoenix
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