Three different test tubes were used to test for the effect of alkalinity and temperature on the structure of DNA. The control of the experiment was test tube A because no variables were tested. This test tube was necessary in the experiment because it behaved as a baseline to compare with the results of the experimental variables. Test tube B was exposed to a high temperature (100°C) to observe the effect of high temperatures on DNA structure while test tube C was exposed to an alkaline pH through
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0225mM. keeping our enzyme constant at 100μL, what we concluded is a significant decrease on the absorbance as we decrease the substrate concentration, for example our initial reading of absorbance at 0.09mM concentration was 0.558, the same reading at 0.0225mM was significantly lower (0.176) thus indicating that substrate concentration does influence absorbance if the enzyme concentration remains constant.
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The purpose of this lab was to determine the effect of temperature and pH on the reaction rate of Catecholase. The substrates of catecholase are catechol and oxygen, and it is an enzyme that speeds up chemical processes. Enzymes work best under optimal conditions, and we tested the reaction rate of catecholase with substances containing different temperatures and pH levels. pH measures the acidity or alkalinity of a substance, and is measured on a scale from 1 to 14. Temperature is the measurement
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ADAMTS-13 is a protease and it’s role is to cleave vWF (ulvWF). ADAMTS-13 INH ELISA allows us to detect the human autoantibodies (IgG) which is found in plasma and or serum against the ADAMTS-13. These autoantibodies inhibit ADAMTS-13 activity, hence non-cleaved ULvW gather in the plasma – one of the main causes of thrombotic thrombocytopenic purpura (TTP). TECHNOZYM® ADAMTS-13 INH test allows us to differentiate between autoantibodies (acquired) and congenital (gene polymorphisms) TTP when paired
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ANSWER THE FOLLOWING QUESTIONS (ignore questions in lab manual) 1) Describe clearly and precisely the function of a positive control. 2) Describe the function of a negative control. What conclusions you would make about your entire experiment if the negative control did not act accordingly? 3) What might you conclude about the results of your test samples if one or more of the test samples tested positive but the positive control did not work according to plan? 4) What tubes represent the positive
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Introduction: This unit, about Enzymes and Biotechnology, contains information on how enzymes work in chemical reaction. For every enzyme there is a substrate made of reactant molecules that is worked upon. During a chemical reaction, the enzyme lactase breaks lactose molecules into their monomers, glucose and galactose. This lab is observing enzyme specificity as demonstrated by how lactase affects the presence of glucose in soy, cow, and rice milk. Data: Conclusion: 1. Cow milk contains lactose
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honor code of University of the Pacific. (Please sign by typing your name or pasting an electronic signature if you have one) Signature: Jennae Fenton Date: 10/12/17 Abstract: The enzyme catechol oxidase is a protein that produces benzoquinone when the substrate catechol binds to the enzyme. This is commonly referred to as browning. How quickly produce spoils
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Background Dexcom is a medical device company based out of San Diego that uses innovative technology to improve the quality of life for diabetics across the globe. Dexcom is transforming diabetes management by providing superior continuous glucose (CGM) technology to help patients, and healthcare professionals better manage diabetes. Dexcom was founded in 1999, and went public in 2005. Kevin Sayer is the current CEO, and is on the board of directors with eleven others majority of whom have significant
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The first article discusses the gymnotiform fish Eigenmannia, a weakly electric fish, which generates a weakly electric signals for electrolocation and communication. Metzner provides explanation as to how the signals are produced and mentions the electric organ discharges (EODs) that are driven by a medullary pacemaker nucleus. The electrolocation can suffer from dramatic degradation when the conspecifics with a similar frequency are present in the environment. To avoid jamming, Eigenmannia modulates
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In biology, in order to purify biological molecules, a common process called chromatography is often used. This process involves the separation of the large particles in the solution from the smaller particles. This is instrumental in filtering the solution, but without specialty scientific instruments, there is no way to immediately know the concentration of the, now separated, solution. However, there are multiple processes that are utilized to find unknown concentrations of a solution. One of
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