Data: See attached sheet Discussion: The catalase enzyme does not work at high temperatures around or above 50 degrees celsius. We proved this in our lab when the slope changed from 0.021 kPa/sec at 34 degrees celsius to negative 0.009 kPa/sec at 54 degrees celsius. With just a change in 20 degrees the slope, or how well the enzyme is working went from really good to really bad. The catalase enzyme works best around a pH of 10. At a pH of 4 and 7 the slopes were 0.007 and 0.009 kPa/sec. At the
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Enzymes are fundamental proteins which act as effective catalysts for biochemical reactions within an organism. Enzymes are distinct from one another; they attach themselves to specific slots on substrates called active sites to lower the activation energy to start the chemical reaction. This is represented by the lock and key model, where the shape of the enzyme directly corresponds to the substrate to carry out a specific job. An enzyme is able to be used until it becomes denatured, or when the
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Enzymes are biological catalysts or assistants that consist of various types of proteins that work to drive the chemical reaction required for a specific action or nutrient. They can either launch a reaction or speed it up. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). The catalase used in this experiment will come from five different sources: Spinacia
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Biology HL 20/9/2013 How Substrate Concentration affects a Catalase Enzyme Reaction RQ How does changing the substrate concentration affect the rate of a catalase reaction in an enzyme? Hydrogen peroxide was used as the substrate and the rate was measured by oxygen production. Hypothesis If the substrate concentration is increased then the rate of a catalase reaction will also increase until it reaches the optimal concentration or saturation point and will remain constant. This is because there
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Running head: LAB 5: INVESTIGATING AN ENZYME-CATALYZED REACTION Lab 5: Investigating an Enzyme-Catalyzed Reaction September 24, 2014 Principles of Biology 120.601 Mrs. Annemarie Duncan Abstract: (Burmania) This experiment was performed in order to examine ways in which a potato catalase enzyme reacts to various assays with differing variables. To do so a baseline assay (undiluted extract and room temperature H2O2) was used within the experiment with only one other variable changed in
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the rest of reaction tubes. (2 marks) |No of the reaction tube |Solution |Material |Relative amount of gas produced during the first two| | |(substrate + buffer) |(enzyme source) |minutes after the reaction started | |1 |3ml H2O2 + 3ml DDW |Fresh potato |Potato remained at bottom of test tube, | |
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morphology after Gram staining and observed under a brightfiled microscope: purple color, spherical shape and clustered like grape. After determining that it was a Gram positive staphylococcus, it was inoculated on a MSA plate, Blood Agar, DNA agar and Catalase test was also performed to help figure out the staphylococcus type. The Table below lists all of the biochemical tests, their purpose and results. TEST PURPOSE REAGENTS OBSERVATIONS RESULTS Gram Stain To determine the Gram reaction of the bacterium
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Effect of Catalase on Hydrogen Peroxide Introduction Catalase is an oxidative catalyst enzyme that is found in all organisms that require oxygen or can survive in its presence. Catalase is an enzyme which catalyzes the breakdown of hydrogen peroxide into oxygen and water (Everything Bio, 2007). Catalase is found in large amounts in the liver and serves as the liver’s cleansing function. Hydrogen peroxide is a colorless, dense liquid that is often used as bleach or is diluted with water for
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4/15/2015 BIO156 Lab 2 Print Lab 2 Biological Molecules and pH Introduction: Connecting Your Learning Biological organisms, like all things, are made up of elements. These elements combine to form organic molecules that create the basis for life. The main elements found in biological organisms include carbon (C), hydrogen (H), phosphorus (P), nitrogen (N), and oxygen (O). This lab describes how these elements form some of the most important molecules in life: carbohydrates, proteins
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[pic] FHSB 1214 BIOLOGY 1 FOUNDATION IN SCIENCE [pic] |Name |Chai Che Hoe | |Group member |Lim Chen Ye | | |Liow Yi Sheng | |
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