DL3: Instructor Newton 3/29/2015 PURPOSE This experiment is designed to become familiar with and perform the MR-VP biochemical test, learn variations in how different organisms metabolize glucose, and to become familiar with and perform the catalase biochemical test. Materials Used 10% bleach solution Hydrogen peroxide Paper towels Saved E. coli culture Stock culture: S. epidermidis Gloves Candle used for a flame source Test Tube Test Tube rack Pipet Slide-Box-MBK with blank
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Lab Background: Enzymes are proteins that catalyze or control metabolic reaction. Enzymes work by lowering the amount of activation energy needed so the reaction will happen more quickly. The molecules that an enzyme acts upon are called substrates, the substrate solutions used in this lab were milk and water. In this lab, the enzymes are specific for particular substrates. The enzyme (junket tablet) converts these substrates into different molecules by curdling. If the enzyme concentration required
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The purpose of this laboratory exercise was to perform tests necessary to be able to distinguish one microorganism from 10 others. Using a series of biochemical tests and characteristics, unknown #22 was concluded to be Pseudomonas aeruginosa. A dichotomous key was mapped out and used during this process. Using this provided guidance as well as organization as to what the result may be. Upon obtaining the unknown organism, it was important to make a streak plate of the bacteria on TSA. The purpose
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A lysosome (derived from the Greek words lysis, meaning "dissolution/ destruction", and soma, "body") is a membrane-bound cell organellefound in most animal cells. They are known to contain more than 50 different enzymes, which are all optimally active at an acidic environment of about pH 4.5 For this function they are popularly referred to as "suicide bags" or "suicide sacs" of the cell. hey were discovered and named by Belgian biologist Christian de Duve, who eventually received the Nobel Prize
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for oxidase and catalase were also performed using an oxidative reagent and hydrogen peroxide, respectively. The Enterotube II System was used to further classify its metabolic profile. The stains revealed that the bacterium was a Gram-negative bacillus. The organism was shown to be non-motile and grows best at a pH of 10 and a temperature of 25° C, making it an alkaliphilic mesophile. The oxidation and fermentation tests showed that the organism is an oxidase-positive, catalase-positive aerobe that
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experiment is looking for amylase in-particular. The first control was a negative control, Water ,this would show there is no Amylase present. The second control would be a Positive control ,the starch solution this would show the presence of the enzyme amylase. The negative control water does not contain starch. Saliva contains Amylase this is why it is included in the experiment. The saliva is a positive because it turned yellow, this shows no starch left because the amylase broke it all
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Your Full Name: UMUC Biology 102/103 Lab 4: Enzymes INSTRUCTIONS: * On your own and without assistance, complete this Lab 4 Answer Sheet electronically and submit it via the Assignments Folder by the date listed in the Course Schedule (under Syllabus). * To conduct your laboratory exercises, use the Laboratory Manual located under Course Content. Read the introduction and the directions for each exercise/experiment carefully before completing the exercises/experiments and answering
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feathers, hooves, silk, and fingernails * Collagens – found in tendons, bone, and other connective tissue * Elastins – found in blood vessels and ligaments * Myosins – found in muscle tissue * Fibrin – found in blood clots Enzymes * Enzymes serve a wide variety of functions inside living organisms. They are
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Introduction Enzymes are protein molecules that catalyze chemical reactions in all living organisms. Enzymes allow living organisms to carry out complex chemical activities at low temperatures, but can’t cause a reaction that hasn’t occurred in their absence. Also, enzymes are thought to speed up reactions by bringing reacting molecules together to increase the chances that a reaction will occur (Worthington Biomedical Corporation, 2015). Each enzyme has a specific active site where the substrates
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features commonly seen in the prokaryotic world. Instead, biochemical testing has been used to make bacterial identification down to the “species” level. These schemes are based on creating and matching biochemical profiles of the production of enzymes, acids and gases by isolated pure cultures of a given microorganism. Identification schemes and flow charts can be found in reference texts such as “Bergey’s Manual of Determinative Bacteriology” or “The Prokaryotes”. Each group of students will
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