Part 1 - Sample Preparation The key to a successful identification is to start with a "good" sample. Many pathogenic bacteria do not grow well on solid culture medium, making identification by traditional means difficult. (Why?) Even poorly growing bacterial cultures, however, can be identified with the methods used in this lab. In this lab, you will act as a pathologist or perhaps a pathology lab technician at a well-equipped research hospital. Your task is to identify a bacterial sample received
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1.2.1 STRUCTURE OF GENOME 3 1.2.2 GENETIC VARIATION 6 1.2.3 GENOTYPIC DIFFERENCES 8 1.3 RNA DEPENDENT RNA POLYMERASE ACTIVITY 9 1.3.1 POLYMERASE FUNCTION 9 1.3.2 MODEL SYSTEMS OF HCV REPLICATION 11 1.3.3 GENOTYPE SPECIFIC STUDIES 11 1.3.4 BIOCHEMICAL PROPERTIES 12 1.4 KUNJIN VIRUS RNA DEPENDENT RNA POLYMERASE 13 1.5 CONCLUSION 15 1.6 AIMS AND HYPOTHESIS 16 2 MATERIALS AND METHODS 17 2.1 HCV POSITIVE SERA SAMPLES
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PCR also known as polymerase chain reaction, it was developed by Kary Mullis in 1983 in order to magnify small segments of DNA. PCR is a technique used to produce millions of copies of a particular DNA sequence in less amount of time than previous methods. It became important in the identification of bacteria and virus, diagnosis of disease, genetic manipulation, forensic science, cloning, and in other fields. PCR takes advantage of different temperatures while it mimics the natural process of
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investigate the allele frequency of the PTC taster gene (TAS2R38) in a small population, represented by the students in class. To determine the genotypic profile of the students PTC gene,a sample of DNA was extracted using Chelex resin. Polymerase chain reaction was then used to amplify then digest the gene of TAS2R38 gene with a restriction enzyme. Digested DNA will be cut into 2 fragments 239 bp and 64 bp in length. (Figure 2) This discrepancy in length allowed us to profile the alleles using
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1.2.1 STRUCTURE OF GENOME 3 1.2.2 GENETIC VARIATION 6 1.2.3 GENOTYPIC DIFFERENCES 8 1.3 RNA DEPENDENT RNA POLYMERASE ACTIVITY 9 1.3.1 POLYMERASE FUNCTION 9 1.3.2 MODEL SYSTEMS OF HCV REPLICATION 11 1.3.3 GENOTYPE SPECIFIC STUDIES 11 1.3.4 BIOCHEMICAL PROPERTIES 12 1.4 KUNJIN VIRUS RNA DEPENDENT RNA POLYMERASE 13 1.5 CONCLUSION 15 1.6 AIMS AND HYPOTHESIS 16 2 MATERIALS AND METHODS 17 2.1 HCV POSITIVE SERA SAMPLES
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BIOTERRORISM Name: 1. Introduction Historically, infectious disease outbreaks brought about by microbial species against human beings have caused far more mortality rates than war itself. Numerous cases of disease outbreaks in the past have been a major concern to health authorities, although such concerns have been partially addressed in the recent past. Examples of disease outbreaks in the past include: the infamous Bubonic Plague of the 14th century in Europe that led to the death
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Identification of Chemotaxis Protein Substrates in Thiomicrospira crunogena Introduction Thiomicrospira crunogena is a Gram negative, aquatic, colorless sulfur oxidizing, chemolithoautotrophic bacterium. Cells are spiral shaped 0.2-0.3 µm in diameter and 1-2µm long, some individual cells can reach up to 30 µm long (3). T. crunogena is motile via a singular polar flagellum. It is the first deep-sea autotrophic hydrothermal vent bacterium to have its genome fully sequenced and annotated
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Lester Minhenga Ngijoi 2/21/18 Dr. Chad R. Sethman Abstract DNA fingerprinting is the process of analyzing an individual’s DNA base-pair patterns. The DNA fingerprinting lab involved identifying the suspect using Agarose Gel and Polymerase Chain Reaction. It was found that suspect two s DNA matched the crime scene DNA. This is known because suspect twos DNA traveled the same distance as the crime scene DNA. DNA Fingerprinting Using Agarose Gel Introduction In 1984 Dr. Alex Jeffreys
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Andrei Vasiliev 01040632 PTC Genotype Determination basing on DNA Samples (Obtained From Individuals with known PTC Test result) that are incubated with Restriction Enzyme (Hae III) Abstract: The ability to taste the bitter compound phenylthiocarbamide (PTC) and related chemicals is bimodal, and all human populations tested to date contain some people who can and some people who cannot taste PTC. Why this trait has been maintained in the population is uncertain but this polymorphism may influence
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rising number of cancer-related deaths. The global cancer diagnostics market is witnessing a rapid transformation owing to several technological advancements in diagnostic platforms such as next-generation sequencing, DNA microarrays, and polymerase chain reaction (PCR). Moreover, the market has seen the advent of hybrid imaging instruments with enhanced accuracy, such as PET/CT and SPECT/CT. These advancements have augmented the growth of the cancer diagnostics market. See Complete Info at: http://www
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