...GOLD BUYING PROCEDURE Undocumented/treasure AU Bars PRIOR OR BEFORE IMPLEMENTATION A TABLE MEETING IS A MUST FOR M.O.A SIGNING BY BUYER & SELLER. For sponsored project (Seller is not the one paying the Contingency Fee/ Intermediary/ies paid the fee ) both Parties have agreed that a day before implementation, First Party (SELLER ) will hand over the _____ grams of sliced gold grams from subject gold bars to Second Party ( BUYER ) as “commitment” and to be given to the buyer’s verifier at the venue a day prior ( before ) to implementation as “COMMITMENT” rest in three MAJOR reasons: Assurance gold slice quantity varies as follows : METRO MANILA 100 grams LUZON 200 grams VISAYAS & MINDANAO 300 grams. 1. Avoid last minute back out. ( pag-urong ng seller sa araw ng bilihan / paglabag sa pinirmahang kasunduan ) 2. Avoid switching ( pagpalit ng item na peke ) 3. Serve as key for the Buyer as assurance for expenses incurred ( in caase of seller’s failure ) ( ang sliced grams of gold ay magsilbing pantapal sa nagastos ng BUYER, kung sakali man magkaroon ng hindi pagkatuluyan sa transakayon sa anumang dahilan nilabag ng SELLER sa araw ng buying/implementasyon. ) MIMINUM BUYING PER IMPLEMENTATION 6.2 KG. ( 5 BARS ) 12.5 KG ( 3 BARS ) 74.6 KG. ( 1 BAR ) Transaction/s wherein the Seller or intermeiaries pays for the Contingency Fee for its implementation...
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...culture, is the removal of cells from an animal or a plant, and growing the cells in a favourable artificial condition (Wiley, J.M et al, 2013). There are three types of cell culture: primary, this is cultivating the cells away from the parental tissue, secondary, which is when a primary culture is sub-cultured and cell lines, which can be continuous or finite depending on the life span of the culture. Virus titrations are used to estimate the virus concentration; it is a viral quantification technique. When detecting the virus, the cytopathic effect is looked at, whether there is lysis of the cells, vacuolation, formation of syncytia and the presence of inclusion bodies. TCID50, is the measure of the infectious titre. The end point dilution assay quantifies the amount of virus that is required to kill 50% of infected hosts or to produce a cytopathic effect in 50% of inoculated tissue culture cells (Kumar P, 2013). The purpose of the virus titration within tissue culture is to isolate and identify viruses within clinical samples, to carry out research on the viral structure, replication, genetics of the virus and the effect on the host cells, and also to prepare viruses for vaccine production. Results Table 1 Data used to determine the 50% endpoint using the Reed-Muench method Log of virus dilution | Infected test units | Cumulative infected (A) | Cumulative non-infected (B) | Ratio A/(A+B) | Percentage infected (%) | -1 | 5/5 | 37 | 0 | 38/38 | 100 | -2 | 5/5 | 32 | 0...
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...Introduction Ash is a vital ingredient in the canine diet as it is the main constituent of minerals within most diets. Ash content in food is determined by burning the food to calculate the ash percentage left behind. A deficiency in any of the minerals within ash would result in a wide range of health problems which is why it is a legal requirement to display the ash content on food packaging. This experiment is designed to recreate the process undergone by the pet food company to determine the ash content within the food. Method Weigh silica crucible and record weight prior to experiment. Grind over 5g of food sample in pestle and mortar and measure 5g of ground sample into the crucible. (W0) Place the dish in a baking oven for 24hrs to dry out the sample. Reweigh the sample in silica crucible, This is now the true weight of the sample (W1) Set up the bunsen burner on top of the heatproof mat with tripod and clay pipe triangle above to support the crucible. Ignite the bunsen burner and begin to heat the sample slowly ensuring the sample does not set on fire as this will result in a loss of material to the atmosphere in the form of particles. The sample should turn black. Once the sample reaches roughly 100°C continue to heat to 500°C until the sample turns white. Allow sample to cool to room temperature in a dessicator. Weigh the finished sample (W2) Calculate the ash content (X): (W2-W0)/(W1/W0)x100=X Materials: Silica dish Baking Oven Bunsen Burner ...
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...4110065A.qxp 9/25/2007 2:39 PM Page 1 ™ Quick Start Bradford Protein Assay Instruction Manual For technical service call your local Bio-Rad office, or in the US, 1-800-4BIORAD (1-800-424-6723) 4110065A.qxp 9/25/2007 2:39 PM Page 5 Table of Contents Section 1 Introduction 1 1.1 Principle 1 1.2 Selecting a Protein Standard 5 1.3 Product Description Section 2 Instructions 9 11 2.1 Standard Assay Protocol 11 2.2 Microassay Protocol 14 Section 3 Data Analysis 18 Section 4 FAQs and Troubleshooting 22 Section 5 Ordering Information 26 Section 6 References 28 Section 7 Appendix 30 4110065A.qxp 9/25/2007 2:39 PM Page 7 Section 1 Introduction The Quick Start Bradford protein assay is a simple and accurate procedure for determining the concentration of protein in solution. It provides ready-to-use convenience by supplying the dye reagent at 1x concentration and two protein assay standards at seven prediluted concentrations. The prediluted standards are conveniently packaged in 2 ml screwcap vials, eliminating wasteful and sharp ampoules, and ensuring protein stability over the shelf life of the product. 1.1 Principle The Bradford assay is a protein determination method that involves the binding of Coomassie 1 4110065A.qxp 9/25/2007 2:39 PM Page 8 Brilliant Blue G-250 dye to proteins (Bradford 1976). The dye exists in three...
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...Animal Nutrition Lab Reports FdSc: Animal Health and Welfare STE09288743 Practical 1 - Determination of the ash content of foods Introduction Foodstuffs are divided into five categories; crude protein – the total level of nitrogen in a feed, ether extract – the total fat content of a feed, nitrogen free extract – the soluble carbohydrate in a feed, crude fibre - cellulose, fibre with nitrogen attached & alkali insoluble lignin and total ash content – an indication of the mineral content of feeds. The aim of this report is to try and find out the total ash content of Wainwrights Adult Complete Dog Food With Duck And Rice through a food analysis method and compare the amount found with what the original packaging states. Mclements (2003) explains that “ash is the inorganic residue remaining after the water and organic matter have been removed by heating in the presence of oxidizing agents, which provides a measure of the total amount of minerals within a food.” It’s important for the correct amount of ash to be in a dog’s diet because as Professional Pet Foods (2013) explain, “excess ash will contribute excess minerals to the diet, potentially leading to health problems.” Method This method can be used for all types of food containing less than 50% fat, it allows for the determination of the mineral content of foodstuffs. In this process, the samples temperature is taken to 550°C to ensure all organic matter is...
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...Baghpat Crossing, MEERUT 250 005 UP, India 2 Department of Pharmaceutics, Laureate Institute of Pharmacy, KATHOG 177 101 The. Dehra, Dist. Kangra, HP, India Abstract: Piper betle leaves (Family: Piperaceae) have been used in Chinese and Indian folk medicine for centuries and recently been proposed to be used as a chemopreventive agent because of its anti-oxidant activity. In the present paper, Piper betle leaves were standardized for stomatal index, vein islet and vein termination numbers, palisade ratio, UV fluorescence and different ash values. The Piper betle leaves are earlier reported to possess anticancer potential. Hence, the aqueous extract of the leaves was subjected to cytotoxicity studies on Hep-2 cell line using MTT and SRB assays. The mean CTC50 was found to be 96.25 µg/mL, which proved the potent cytotoxicity and hence, the probable anticancer property of the selected extract. Key words: Betel Leaf Anticancer Phytochemical characterization and its leaves, with a strong pungent and aromatic flavour, are widely consumed as a mouth freshener [4]. The leaves are credited with wound healing, digestive and pancreatic lipase stimulant activities in the traditional medicine [5]. During our exploration of non-toxic and affordable herbal medicinal formulations, the PBL extract and its constituent phenolics were found to show impressive anticancer activities [6-8]. The deep green heart shaped leaves of betel vine are popularly known as Paan in India [9, 10]. MATERIALS AND...
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...have higher peroxidase activity levels because it was under more stress than the plant that was watered with water. Through the use of such techniques as tissue prints, dot-blot assays, electrophoretic analysis, and spectrophotometric assays, peroxidase activity levels were visualized and quantified. The results were as expected; the tissue from the plant that had been treated with Vitamin Water was more purple on the tissue prints and it was spectrophotometrically determined that the plant that had been treated with Vitamin water had a higher amount of peroxidase activity than the one that was treated with water. The results proved that the hypothesis was correct; stress on a plant, Vitamin Water in this case, causes higher levels of peroxidase activity. Introduction: Peroxidases are enzymes that use hydrogen peroxide as an electron acceptor to catalyze oxidative reactions. Peroxidase is believed to play a major role in synthesizing cell walls, preventing toxins and responding to stress. It is responsible for the purple color that is seen throughout the experiments. Peroxidase can be found in different locations in a plant in the form of isoenzymes. Many different experiments can be done to locate, visualize and quantify the activity of peroxidase enzymes. These include, tissue printing, dot-blot assays, electrophoresis, and spectrophotometry. This knowledge of what peroxidase does and how its activity levels can be detected was used to develop...
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...cancer [2]. Chemotherapy is restricted by both intrinsic and acquired cell resistance to drugs. [3] This has necessitated the use of natural products for the treatment of cancer. There are compelling evidences for experimental investigations on the efficacy of plant drugs against cancer [4]. It is known that there are links between the inflammatory and nociceptive, oxidative and cancer processes. The ability to inhibit any of the process will definitely lead to the inhibition of the others [5]. Based on the fact that M. calabura fruit possess antioxidant activity, the aim of the present study was to determine the in vitro anticancer activity of methanol extracts of the fruits of M. calabura against HeLa and HEp-2 cancer cell lines using MTT assay. Plant Material and Extraction Collection and authentication of fruits of M. calabura Linn were collected from the surrounding areas of Erode, Tamil Nadu state, India during 2008-2009 and the plant was identified and authenticated and deposited (voucher number: BSI/SC/5/23/09-10/Tech-132) at Tamil Nadu Agricultural...
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...The Brad assay * is one of the most common forms of measuring absorbance through a spectrometer * uses a a dye (coomassie Brilliant Blue G-250) that binds to proteins and is absorbed at a wavelength of 465- 598 nm * Fast, therefore can meaure a 96 plate in 5 minutes * Absorbance shift allows the measure the protein at the 598 nm wavelength by using the spectrometer, can be justified through the beer-lambert law * The standard curve can be used to estimate the concentration of an unknown protein in solution by * Taking in account their known absorbance levels * Use line equation from curve * The unknown protein conc. in a sample with a known absorbance level can be determined Bradford Vs. Lowry * Lowry * Uses alkaline conditions- reduction cu 2+ to Cu+ * Folin phenol reagent- yellow to blue * Peptide bonds for monocovalent copper and radicals of try and trp react with folin * Concentrations 0.1-1.0 ug/ml of protein * Can be interfered with easily * Slow 40-60 * Colour varies with proteins * Criticial timing with procedure * * Bradford * Based on absorbance shift from brilliant blue when bound to proteins * 465- 598 (when bound) * Acidic conditions * Binds tightly to protein, and inhibits binding sites (little inference) * Limitations * Strong basic buffers Detergents interfere with binding of the cosmic blue, causing...
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...4PY013 MOLECULAR BASIS OF LIFE PROTEIN ASSAY BY BIURET AND FOLIN METHODS Student Number and Name:1316740 Katerina Koupa | Name of Lab Instructor: Dr. J. Walton | Date of the experiment:06/02/2014 | Date of Report Submission:20/02/2014 | (Fig.1) Biuret Reagent Structure ABSTRACT: This report describes the use of the Biuret and Folin methods of protein assay, to verify the protein concentrations of two unknown solutions. To calculate the protein concentrations of the unknowns, nineteen samples were passed through a spectrophotometer which twelve of them had a known protein concentration and there absorbance levels were found. Then a calibration graph was plot that determines the concentration levels of the unknown samples. INTRODUCTION: One of the most typical procedures applied by lab scientist is the protein quantification. Finding protein concentrations in solutions is important in many ways. First of all detecting the levels of a protein in body fluids can result in identifying various diseases. Also protein verification is required for characterization and purification of enzymes. Because of the significance of protein assays laboratories perform this techniques on almost a daily basis. For this experimental procedure, the Biuret and Folin methods were used to determine the protein concentrations of two unknown samples. First the Biuret method (Fig.1) is based on the appearance of peptides bonds in proteins...
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...Proof that Jesus Christ is GOD Revelation 1:8 I am Alpha and Omega, the beginning and the ending, saith the Lord, which is, and which was, and which is to come, the Almighty.(kjv) (Jesus calls himself the alpha which is the beginning of everything and the omega which is the end of everything he also calls himself the almighty these are all names of GOD) John 13:13 Ye call me Master and Lord: and ye say well; for so I am. (John 13:13 KJV) Isaiah 42:8 I am the Lord: that is my name: and my glory will I not give to another, neither my praise to graven images. (Isaiah 42:8 KJV) Matthew 28:18 And Jesus came and spake unto them, saying, ,All power is given unto me in heaven and in earth. (Matthew 28:18 KJV) John 14:1 Let not your heart be troubled: ye believe in God, believe also in me. (John 14:1 KJV) John 8:23 And he said unto them, Ye are from beneath; I am from above: ye are of this world; I am not of this world. (John 8:23 KJV) Exodus 3:14 And God said unto Moses, I AM THAT I AM: and he said, Thus shalt thou say unto the children of Israel, I AM hath sent me unto you. (Exodus 3:14 KJV) John 8:58 Jesus said unto them, Verily, verily, I say unto you, Before Abraham was, I am. (John 8:58 KJV) John 14:8:9 Philip saith unto him, Lord, shew us the Father, and it sufficeth us. Jesus saith unto him, Have I been so long time with you, and yet hast thou not known me, Philip? he that hath seen me hath seen the Father; and how sayest...
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...History 200 14 December 2010 1 Mysticism and Diabolic Witchcraft: Female Susceptibility of the Italian Renaissance During the Italian Renaissance, Christianity experienced a heavy resurgence in mysticism. Mysticism was a type of devout faith or spirituality found throughout the convents in Italy and primarily exercised by Christian Italian women (Sheldrake 93-95). These women underwent vivid connections with God which involved an awakening of consciousness and awareness for God’s divine will. In extreme cases, women fell into a transcendental union with God in which they experienced ultimate illumination. In these rare occurrences, women could encounter faith miracles such as stigmatas, ecstasies, or the re-living of Christ’s Passion. During this period, Italy also experienced another intense spiritual movement labeled diabolic witchcraft (Tavuzzi 150). In the case of diabolic witchcraft, again experienced primarily by females, women underwent a concentrated level of worship and contractual relations with Satan. Historical examples show these women developing sexual relations with Satan, as well as maleficia or harmful magic (Tavuzzi 153). The women involved in diabolic witchcraft were pursued by the Church’s legal arm, the Dominican Inquisitors. They were put on trial, accused of heresy, and either imprisoned or killed. Similarly, the Dominican Inquisitors investigated women who were involved in mysticism and upon the examinations performed by the inquisitors;...
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...is today. This paper will discuss the historical connections, theological similarities, and contemporary struggles between Judaism, Islam, and Christianity as well as the contemporary struggles within Judaism. Theological Similarities Judaism, much like Christianity and Islam, shares a common belief in one God, believes in sacred time, prophets, angels, Satan, and divine revelation. Jews, Christians, and Muslims worship the same God, but call him by a different name. These three faiths believe in peace. In fact, the three religions have different greetings that wish peace upon others proves their similarities regarding peace. Jews, Christians, and Muslims believe in accountability, moral responsibility, judgment day, eternal punishment, and eternal rewards. The theological similarities shared among the religions do not stop at their common beliefs, but where they get them from. All three faiths are ‘Abrahamic’ (Robert Marquand, 1996). Judaism, Christianity, and Islam were conceived from Abraham. All three faiths emphasize the special covenant with God, for Judaism through Moses, Christianity through Jesus, and Islam through Muhammad (Esposito, 2009). Though founded by different people, these three faiths were taught with similar messages by different individuals seen as prophets. In fact Judaism, Islam, and Christianity share the common names of Abraham, David, Solomon, Jesus, Moses, and Mary the mother of...
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...Christianity: Pictures and Controversial Captions As Christians rally to spread the Gospel of Jesus Christ, they are faced with a multitude of controversy. One example: photos depicting Jesus Christ upon the cross with mocking captions below it: as seen in this particular photo. CHRISTIANITY The belief that a cosmic Jewish Zombie who was his own father can make you live forever if you symbolically eat his flesh and telepathically tell him you accept him as your master, so he can remove an evil force from your soul that is present in humanity because a rib-woman was convinced by a talking snake to eat from a magical tree… yeah, makes perfect sense now. To most followers of Christ the photo alone is representative of a beautiful sacrifice. We all know the story of how Jesus Christ died for our sins. In accepting this concept as followers of Christ we are to be permitted into the “Kingdom of Heaven” where we will have an everlasting joy filled existence, upon His return. This is only offered to those who truly believe. Sadly there are many who are only “Sunday Christians,” “Christians in times of wants/needs,” “Holiday (Christmas/Easter) Christians” or whenever it’s convenient for them. The bible teaches these are not true followers of Christ, and will not be permitted into the Great Kingdom. This belief has caused non-Christians, Agnostics, Atheists and other non-Christian faiths to try and destroy what Christians hold so dear to them. The integrity of our Father...
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...The religious Awakening of early America proved to be especially divisive because it (a) disrupted the prevailing social order in many towns and cities that utilized a church government, (b) encouraged emotional involvement in religion, and (c) made religion an individual matter. These factors combined to cause division within many of communities of the nascent country. Before the series of events know as the Awakening, many towns and cities had one church, and one preacher; who essentially had a monopoly on the local church goers. Not only were the people united religiously, but in many towns, the church was also the government. It was this in this state that traveling preachers know as itinerants found many of the communities they sought to evangelize. The itinerants traveled from place to place preaching doctrine that generally contrasted with what local leaders taught. At times, groups of them would enter a town, and in a swirl of religious fervor, win the people’s religious loyalty away from the existing order. This not only angered local leaders, but, also tended to divide local populations, as some believed the new doctrines that were preached, and others vehemently opposed the new ideas. Those who believed the itinerants were called the “new lights,” and those who stuck to the old ways were referred to as “old lights.” These theological differences became a serious problem in many communities because religion was also the main social structure. Those who came to...
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