...WORKING DILUTION PROBLEMS It is a common practice to determine microbial counts for both liquid and solid specimens--suspensions of E. coli in nutrient broth all the way to soil samples and hamburger meat. Most specimens have high enough numbers of microorganisms that the specimen has to be serially diluted to quantitate effectively. The following is a step-by-step procedure to working dilution problems, and includes some practice problems at the end. The purpose can be determination of bacterial, fungal, or viral counts (indirectly). This protocol is specific for bacterial counts (colony-forming units, CFUs), but can be modified for fungi (CFUs) and viruses (plaque-forming units, PFUs for viral counts). A set of serial dilutions is made, a sample of each is placed into a liquefied agar medium, and the medium poured into a petri dish. The agar solidifies, with the bacterial cells locked inside of the agar. Colonies grow within the agar, as well as on top of the agar and below the agar (between the agar and the lower dish). The procedure described above produces a set of pour plates from many dilutions, but spread plates (sample spread on top of solidified agar) can be used also. The agar plate allows accurate counting of the microorganisms, resulting from the equal distribution across the agar plate. This cannot be done with a fluid solution since 1) one cannot identify purity of the specimen, and 2) there is no way to enumerate the cells in a liquid. Bacterial colonies Viral...
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...Part I INTRODUCTION 1.1 Theories/Concept of the Design Class Volume Monitoring System with Automatic Influx Adjustment is a reliable circuit that takes over the task of counting the number of individuals inside a room very accurately. When somebody enters the room, the counter is incremented by one. When somebody exits the room, the counter will count down by one. The total number of individuals inside the room is displayed on the two 7-segments. Individual Counting is simply a measurement of the individual traffic entering and exiting offices, malls, sports, venues, rooms etc. Counting the number of individuals helps to maximize the efficiency and effectiveness of employees, floor area of an organization, and even the monitoring of the people inside. Individual counting is not limited to the entry or exit point of an establishment but has a wide range of applications that provide information to management on the volume and flow of people throughout a location. A common method for counting the visitors involves sharing human auditors to manually tally the number of individuals who pass by a certain location. But human-based data collection comes at great expense. Our project aims to automate the counting of individuals with the use of a detector called Light-Dependent Resistor which makes it more reliable and efficient. The use of human auditors is redirected to other functions which makes a more productive system. Another feature of this project is...
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...kill it in the middle of the experiment. Method: These are the steps that we followed when doing the experiment: 1. Use a pipette to transfer the daphnia to a cavity slide containing pond water of known volume. 2. Add some cotton fibres to the cavity slide and move them with a fine paint brush in such a way that the daphnia can’t move. 3. Use a calculator to calculate the heart rate. Set its reading to zero and add 1 to the answer. 4. One student will use a stopwatch to count 30 seconds which is the time during which the measurement of the heart rate will take place. 5. Turn the microscope lamp on and locate the heart of the daphnia through the microscope. 6. Measure the beat rate. 7. Repeat steps 1-6 about 4 or 5 times and get an average. Then calculate the heart beats per minute (just by doubling the average). 8. Clean the cavity slide using a tissue and make sure there is no pond water left. 9. Use a pipette to transfer a known volume of caffeine solution of concentration 0.125% to the cavity slide. 10. Transfer the daphnia to the cavity slide...
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...counting FunWithNumbers fun = new FunWithNumbers(); boolean done = false; try { do { fun.displayMenu(); choice = input.nextInt(); switch(choice) { case 1: System.out.print("Please enter the number that you would like to perform the factorial on: "); userNumber = input.nextInt(); System.out.println("The factoral of " + userNumber + " yields the value: " + fun.findFactorial(userNumber)); break; case 2: System.out.print("Please enter the number of fluffy bunnies whose ears you would like to count: "); userNumber = input.nextInt(); System.out.println(userNumber + " bunnies would get you " + fun.bunnyCounter(userNumber) + " ears."); break; case 3: System.out.print("Please enter the number representing the position in the Fibonacci Sequence " +...
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...issues; the governance of decisions that define expectations or verify one's performance-- as well as managing and guiding one's decisions and processes for completing a task, also comes into play; finally, the obligation to report, explain, and be answerable to resulting consequences when implementing those actions is quite significant. To put it simply, when taking accountability for something, you are acknowledging and assuming responsibility for your actions.If I go further in depth with this particular topic, we can see that this system of accountability can actually be created by breaking down the very word "accountability," into separate synonyms (words that have the same or similar meaning) and can be categorized as such. The word "count," in "accountability" implies the action of finding the number of elements of a finite set of objects. The traditional way of counting consists of continually increasing a (mental or spoken) counter by a unit for every element of the set, in some order, while marking (or displacing) those elements to avoid visiting the same element more than once, until no unmarked elements are left; if the counter was set to one after the first object, the value after visiting the final object gives the desired number of elements....
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...Assignment Five Differentiated Assessment By Ashford University The Unit Goal that aligns with the Common Core State Standards Kindergarten students will count from 1 to 100 by ones with at least 70% accuracy ten out of ten times by the end of the first-grading period that is usually a quarter, October 10, 2014. Kindergarten students will count from 1-100 by tens with minimum of 70% accuracy ten out of ten times by the end of the first-grading period, October 10, 2014. How will mastery be measured? Students will demonstrate knowledge by creating snack time refreshments for the entire room, students, staff, visitors if any, all will be the same number of crackers or grapes; even if the diabetic girls cannot eat the prepared snack. As they hand they snack which are to have ten of the main “course” to the individuals, hand them the snack plate, counting by ten as they hand the plate to the recipient. First person, they’ll say ten; next recipient they’ll say twenty; and so forth until ten people have received snack, and the student has reached the 100 by tens goal. Specific Outcome At the end of this quarter, October 10, 2014, you will be able to: prepare snack for a classroom by equal distribution of the main food item, whether it be celery, crackers or grapes. Students’ will decide which snack will be served (motivation) (Puckett, K., 2013, p. 21). They will place ten-snack items on the snack plates for each in the classroom at snack time. Measurable...
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...Introduction Life as we know it is possible for the presence of the same elements repeatedly distributed throughout the matter that surrounds us in nature. These elements form molecules that in turn are the building blocks of every biological cell. Significantly, the information obtained from nature to understand life and evolution are the product of an applied scientific method, which has been improved by the invention and use of technology over time. Nowadays, Scientific are able to measure the components of specific samples precisely, but there are cases when limitations are present in their investigations. For example, that is the case of the measurement of proteins, which is not as easy as looking a sample through a microscope to be able to count how many proteins are present in the biological sample. Proteins are an...
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...Detailed Lesson Plan (Grade 7) 1. Objectives After providing the necessary materials, each student; 1.1 investigates the different types of solutions: -unsaturated -saturated -supersaturated 1.2 performs an actual activity about solubility. 1.3 values the common solutions that can be found at home and can be used in daily living. 1. Learning Tasks 2.1 Topic: Solutions 2.2 Concept: The unsaturated solution has a less amount of solute to be dissolved. The saturated solution can hold no more solute to be dissolved. The supersaturated solution cannot hold more solute. 2.3 Materials 2.3.1 Textbook/Other Reference -Science Grade 7: Matter (K-12 Curriculum), pages 1-16 - http://www.infoplease.com/encyclopedia/science/solution-heat-solution.html 2.3.2 Instructional Materials - video clips, materials enumerated for the experiment and activity sheet. 2. Methodology 3.1 Daily Activities 3.1.1 Prayer/ Greetings Christ Jesus Whom we open our eyes, may you be there; When we open our ears, may you be there; When we open our mouths, may you be there; When we open our diaries, may you be there. Help us to see with your eyes; Help us to hear with your ears; Help us to speak your truth in love; Help us to make time for you… for others… for ourselves. Amen. 3.1.2 Checking of Attendance 3.1.3 Checking of Assignment 3.2 Preparatory Activities 3.2.1 Review Teacher: Class...
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...Osmosis Introduction: Tissues are made up of cells that have the same structure and function. In this experiment osmosis will be measured in a piece of tissue. Water potential can be determined by the changes in dimensions of the tissues when it is placed in solutions of different concentrations. Research Question: What is the effect of sucrose solution on potato cells Hypothesis: Osmosis will increase when the solutions are less concentrated with sucrose Variables: Dependant: Osmosis Independent: Length Materials: 12 large test tubes or specimen tubes with bungs, marker pen, potato chip cutter, scalpel, fine forceps, 3 watch glasses or petri dishes, ruler, 6 labeled beakers one containing 50cm^3 of distilled water and other four containing 50cm^3 of 0.2 mol dm^-3, 0.4 mol dm^-3, 0.6 mol dm^-3 and 0.8 mol dm^-3. Method: 1- Using the potato chip cutter, cut 24 chips from the potato. Cut the chips into 5cm lengths using the scalpel. Be as accurate as possible. Place 4 chips into each of six, labeled test tubes, one test tube for each of the different sucrose solutions. Pour in enough of each respective solution to cover the potato tissue. Put a bung in each one of the test tubes and label this series “potato”. 2- Repeat the method using the apple fruit or turnip root, putting four chips of tissue 5cm long into each of the second series of test tubes. Label these tubes “apple” or “turnip”. 3- Leave the tubes for at least an hour 4- After at least...
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...FUNDAMENTALS OF SURFACE MODES: ¥ COLLOIDS ¥ means ÒglueÓ in Greek ¥ was coined in 1861 by Thomas Graham. ¥ ¥ ¥ ¥ ¥ WHAT IS A COLLOID? usually consists of two phases; one continuous phase in which the other phase is dispersed. Size of particles: larger than the size of molecules and small enough for the dispersed phase to stay suspended for a longer period of time. ¥ No strict boundaries for the size limits. OFFICIAL DEFINITION ¥ In 1903 Wolfgang Ostwald formulated the official definition of a colloid: ¥ a system containing entities having at least one length scale in between 1nm and 1µm. ¥ For smaller particles there is no distinct boundaries between the phases and the system is considered a solution; ¥ for larger entities the particles will fall to the bottom due to the gravitational force, and the phases are separated. 1 2 3 MESOSCOPIC PHYSICS ¥ The particle size is in the so-called mesoscopic range in between the macroscopic and microscopic limits. LARGE INTERFACIAL AREA ¥ One very important quality of the colloids is the large interfacial area between the dispersed and the continuous phases. WHAT EFFECTS HAS THIS? ¥ This means that interface effects and hence the electromagnetic surface modes, are very important for the properties of the colloids. ¥ It costs energy to create this much surface and the particles would clump together if this isnÕt prevented. ¥ Usually the particles are charged and hence repel each other. 5 6 4 Four states...
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...encouraged to ask question/s that is related to this module and that you don’t know the answer to and want it to be answered. The question/s can be answered by anyone in class. The instructor will allow sometime for other students to answer the question/s before contributing. You can post your questions in MODULE 2 forum under the Discussion Forums. Complete Assignment #2. The assignments are posted in the ASSIGNMENTS area of the website. Even though the homework assignments are not to be turned in and graded, you are strongly encouraged to do them to understand the material and to prepare for quizzes and tests. A complete solution to this assignment will be posted on the course website under ASSIGNMENTS on Thursday (Sept 4th). You are encouraged to review the solutions and compare your work to the solution, ensuring that you understand the reasons the solutions appear as they do. You are also encouraged...
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... * Has no secretary The most important problem would be catching the flight for the meeting in Chicago since it’s an emergency and work related. Solutions: Getting work done for the day on the air plane on the way to the meeting. Call the secretary to find out at least some of the info for the meeting. Item#2 Problems: * Internal politicking in the company * Finding someone to replace F.T. Dickenson * Dealing with eliminating his overtime hours The two most important problems would the politicking, because someone else may feel the same way and decide to leave the company too. Also, finding someone to replace him and do his unfinished work in such a short period of time. Solution: Search for a new employee ASAP. Item#3 Problems: * Workers threatening to walk out over a co-worker * 10 votes to dismiss Foreman Edward George The workers are the most important asset to the company, so their interest should be first. Second, you have to figure out what to do about the votes to dismiss Ed George. Solutions: Talk to Ed George about his problems with his co-workers. Hold a meeting and get even more workers involved in the voting process, the take another vote. Item#4 Problems: * Overloading which can result in interruption of electrical power. * Not being reachable for Southern Power Solution: Contact Southern Power ASAP. See if they could come out and help with the problem. Item#5 Problem: * Balancing your work and his until Wednesday ...
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...Name Date Class COLLIGATIVE PROPERTIES OF SOLUTIONS Section Review Objectives • Identify the three colligative properties of solutions • Describe why the vapor pressure, freezing point, and boiling point of a solution differ from those properties of the pure solvent. Vocabulary • colligative properties • freezing-point depression • boiling-point elevation Part A Completion Use this completion exercise to check your understanding of the concepts and terms that are introduced in this section. Each blank can be completed with a term, short phrase, or number. In a solution, the effects of a nonvolatile _______ on the properties of the solvent are called _______. They include _______ point and vapor pressure _______, and boiling point _______. In each case, the magnitude of the effect is _______ proportional to the number of solute molecules or ions present in the _______. Colligative properties are a function of the number of solute _______ in solution. For example, one mole of sodium chloride produces _______ as many particles in solution as one mole of sucrose and, thus, will depress the freezing point of water _______ as much. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. Part B True-False Classify each of these statements as always true, AT; sometimes true, ST; or never true, NT. 11. When added to 1000 g of water, 2 moles of a solute will increase the boiling point by...
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... it increases the water movement out of the plant by a process called transpiration. When there is a shortage of water, the guard cells well get smaller and close the stomata, and the transpiration process will move slower. The osmolarity would be tested with solute concentrations ranging from 0.0 M to 0.6 M. The concentrations increased 0.1 M each time and the solute used was sucrose. The goal of this experiment was to determine which concentration had the least effect on the potato after being incubated. This information helped us estimate the osmolarity of the potato tuber tissue. Our group hypothesis is that the osmolarity of the potato will have the concentration with the 0.1 M solution. This hypothesis is based off the predicted outcome that smaller molarity concentrations will have the least effect on the potato. The more solute added to a solution decreases the concentration of water in most cases (Kosinski). The decrease in water concentration would then lead to a lower weight of the potato tuber once it has finished incubating. Methods & Materials: For experiment A you will need the following items: 1 large potato tuber Forceps petri dish razor blade DI water metric ruler 7 250 mL beakers/ or disposable cups balance that weighs to the nearest 0.01g sucrose...
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...The data solute concentration of the egg was found by taking the average percent change in mass for each percent solute concentration used in the lab and putting the data into the graph(Mass vs. Solute Concentration Over 3 Days). According to the data from the class, the solute concentration inside an egg is 25.1%. The egg would be in an isotonic solution at 25.1% solute concentration. This is when the concentration outside the egg is equal with the concentration of water inside the egg. The point of equilibrium is the point at which the trendline crosses the x-axis. At this point, the egg would no longer gain or lose mass. If the concentrations are the same inside and outside the egg, diffusion will not be able to take place. All averages...
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