...CH 220C ORGANIC CHEMISTRY LABORATORY Spring, 2015 Section Page 1. General Information 2 2. Safety Information 2 3. Attendance 3 Make-Up Policy 3 4. Laboratory Protocol 3 Assigned Reading 3 Pre-Lab Quizzes 3 Lab Notebook 5 Chemicals 5 Due Dates for Reports 5 5. Orientation 5 In-Lab Information 5 Library Information 5 6. Check-In 6 7. Grading Procedure 6 8. Policy on Cheating 7 9. TA Office Hours 8 10. Faculty Course CoordinatorS 8 11. Course Web Page 8 12. Hints to Minimize Frustration IN ORGANIC CHEMISTRY 8 13. Work Schedule 10 Lab Report Due Date Schedule 10 Experiments 10 14. Supplements 17 A. Extraction of Unknown 17 B. Recrystallization of Unknown Products 18 C. Methyl Benzoate 19 D. Synthesis of Luminol 20 E. Azo Violet 23 1. GENERAL INFORMATION PRE- and CO-REQUISITES Pre- and co-requisites for CH 220C listed in the Course Schedule. Important: Because the lecture and laboratory courses are co-requisites of each other, dropping one of them requires that you drop the other as well, unless the drop occurs during ...
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...Experiment 8: Redox: Analysis of the Oxidizing Capacity of Bleach PURPOSE In this experiment, you will show how redox reactions can be used to quantitatively determine the amount of oxidizing agent in liquid hypochlorite household bleach. There are two oxidation-reduction reactions for determining the oxidizing capacity of bleach. Initially an excess of iodide ions are added to a bleach solution. The iodide ions are oxidized to iodine after the solution has been acidified. Starch is added to the resulting iodine solution as an indicator. The solution is then titrated with sodium thiosulfate until the color of the solution changes indicating the endpoint of the reaction. Data collected from the titrations will be used to calculate the mass of the sodium hypochlorite in an unknown solution of bleach. Given the original mass of the unknown solution and the calculated mass of the sodium hypochlorite in the unknown solution, the percent by mass of the sodium hypochlorite can be determined. The oxidizing capacity of the unknown bleach is effectively the percent by mass of the sodium hypochlorite in the unknown bleach sample. OJECTIVES 1) Titration of a sample of bleach with thiosulfate. 2) Determining the end point of the titration with starch indicator. 3) To determine oxidizing capacity of bleach by determining the percentage by mass of the bleach that is sodium hypochlorite. 4) Balance and summarize the two redox reactions to determine the overall chemical reaction that...
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...My statement of “does a solution with a nonvolatile solute have a lower freezing point than a pure solvent?” can be reasoned by the following scientific information. Solutions are composed of a solute and a solvent and most properties of the solution are dependent on the properties of the solute. However, there are four properties that are unique to solutions that are based only on the number of particles of solute in the given solution. These four properties are: vapor pressure, boiling point, freezing point and osmotic pressure and collectively these properties are know as the colligative properties of solutions. In this lab, freezing point depression was observed when comparing a pure solvent of distilled water to a saltwater solution. The decreased freezing point of the saltwater solution is a result of the interactions between particles of solute from preventing the solvent particles from becoming more organized and forming solid crystals. Finally, collected data also confirms that they statement is true. The saltwater solution had an initial temperature of 26.5 °C and after being submersed in an ice bath, the temperature decreased to –5.0 °C. This temperature change was also confirmed when applying the formula for calculating the freezing point of the saltwater solution. The colligative properties concepts as well as the data all support my statement that...
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...Why Animal Testing should be Prohibited Alexandria McConnell 6/4/2015 ENG/130 Maureen Chisholm It is estimated that about 26 million animals are being used every year in the US for scientific and commercial research. They are used for many different things such as medical developments, the safety of products, and many other health care and cosmetic uses. While there are some laws in place that prevent the abuse of animals in these testing laboratories, many of these test subjects face cruel and inhumane treatment. There are alternative and equally effective ways to study and conduct research for such treatments, and although there have been proven benefits to some of the research conducted on these defenseless animals, the harsh effects of the experiments should not be overlooked or ignored. Animals are put through excruciating pain and usually given no anesthesia or relief during many of the test experiments performed in these laboratories. They are often forced to ingest and inhale deadly concoctions to study whether or not it would be safe for human consumption. It is also not uncommon for them to be deprived of food and water for prolonged periods of time. Animal test subjects are also being intentionally injured to study the healing process and the effects of pain and remedies of such, and sometimes purposely killed. A common test performed is the LD50, or lethal dose 50. This test involves giving animal large doses of chemicals to find out how much will kill...
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...Taster Genomic Analysis Lab Report Laboratory Goals: 1. Determine Taster Phenotype 2. Isolate DNA from each individual 3. Determine Taster Genotype Hypothesis: If I am a taster, then my genotype for PTC taster must be either TT (homozygous dominant) or Tt (heterozygous) I – Results: This experiment aimed to investigate the allele frequency of the PTC taster gene (TAS2R38) in a small population, represented by the students in class. The genotype obtained from genomic analysis (via PCR and gel electrophoresis) confirmed that the genotypic result is consistent with the phenotypic result observed at the beginning of the lab. However, DNA fragments of 3 lab subjects didn’t show up on the gel. The allele frequencies can’t be calculated because the data is insufficient to apply the Hardy-Weinberg equation. There are many factors that might be contributed to the invisibility of these DNA fragments, most likely accidental errors. For example, the DNA wasn’t loaded onto the gel probably, or the DNA for some reason didn’t sink to the bottom of the well, or just simply there was not enough DNA. To determine the genotypic profile of the students PTC gene, DNA samplers from each individual was collected from saliva. Using premade PTC primers (short oligonucleotides), a DNA template that encoded the PTC gene (approximately, 303 bp) was amplified by PCR. After amplification, the produced DNA fragments were digested with Fnu4H1 to identify if the lab subjects have a C...
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...Biologically Important Molecules, Carbohydrates, Proteins, lipids and Nucleic Acids Tiffany Osbey Jackson State University Lab Report Authors: Tiffany Osbey Portia Mcmlertrey Jamia Ransome Darrius Bryant Abstract: Organic molecules are those primarily made up of carbon, hydrogen and oxygen. The common organic compounds of living organisms are carbohydrates, proteins, lipids, and nucleic acids. Each of these macromolecules (polymers) are made of smaller subunits (monomers). The bonds between these subunits are formed by dehydration synthesis. This process requires energy; a molecule of water is removed (dehydration) and a covalent bond is formed between the subunits (Fig.1). Breaking this bond is called hydrolysis; it requires the addition of a water molecule and releases energy. Each class of these macromolecules has different structures and properties. For example, lipids (composed of fatty acids) have many C-H bonds and relatively little oxygen, while proteins (composed of amino acids) have amino groups (-NH3+) and carboxyl (-COOH) groups. These characteristic subunits and chemical groups impart different properties to the macromolecules. For example, monosaccharide’s such as glucose are polar and soluble in water, whereas lipids are non-polar and insoluble in water. Objective: Perform lest to detect the presence of biology important carbohydrates, proteins lipids and nucleic acid. Explain the importance of a positive and a negative control in biochemical test. Use biochemical...
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...Studies VII Practical 9 Cell Biology Studies IX Practical 10 Cell Biology Studies X - Experiment Description Page Writing of Lab Reports Identification of Biomolecules 5 13 Identification of Unknown Carbohydrate Solutions and Investigation of Action of Saliva and HCl in Carbohydrate Solution at Two Different Temperatures Investigation of the Effects of Catalase Concentration on Hydrogen Peroxide Decomposition 20 Synthesis of Starch Using an Enzyme Extracted from Potato Tuber Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Microscopy 27 Practical 6 Cell studies II Practical 7 Cell studies III Extraction of Cell Organelles by Cell Fractionation Determination of Solute Potential of Potato Cell Sap 47 Practical 8 Cell studies IV Effects of Different Treatments on Stained Potato Cells 64 Practical 9 Energetics I Respiration of Germinating Beans 67 Microscopic Examination of Cells at Various Stages of Plant Mitosis and Meiosis DNA, Mitosis and Meiosis Modelling 71 Respiration of Yeast 93 Practical 3 Enzyme studies I (Experiment 1) Optional: Practical 3 Enzyme studies I (Experiment 2) Practical 4 Enzyme studies II Practical 5 Cell studies I - - Practical 10 Energetics II Lab manual version 6_201505 FHSB1214 Biology I & FHSC1214 Fundamentals of Cell...
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...Studies VII Practical 9 Cell Biology Studies IX Practical 10 Cell Biology Studies X - Experiment Description Page Writing of Lab Reports Identification of Biomolecules 5 13 Identification of Unknown Carbohydrate Solutions and Investigation of Action of Saliva and HCl in Carbohydrate Solution at Two Different Temperatures Investigation of the Effects of Catalase Concentration on Hydrogen Peroxide Decomposition 20 Synthesis of Starch Using an Enzyme Extracted from Potato Tuber Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Microscopy 27 Practical 6 Cell studies II Practical 7 Cell studies III Extraction of Cell Organelles by Cell Fractionation Determination of Solute Potential of Potato Cell Sap 47 Practical 8 Cell studies IV Effects of Different Treatments on Stained Potato Cells 64 Practical 9 Energetics I Respiration of Germinating Beans 67 Microscopic Examination of Cells at Various Stages of Plant Mitosis and Meiosis DNA, Mitosis and Meiosis Modelling 71 Respiration of Yeast 93 Practical 3 Enzyme studies I (Experiment 1) Optional: Practical 3 Enzyme studies I (Experiment 2) Practical 4 Enzyme studies II Practical 5 Cell studies I - - Practical 10 Energetics II Lab manual version 6_201505 FHSB1214 Biology I & FHSC1214 Fundamentals of Cell...
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...filled bumps. Healthcare Associated (HA) MRSA can lead to more severe illness to the vital organs and can lead to widespread infections. According to Methicillin- Resistant Staphyloccus aureus Infections by John Jernigan and Alex Kallen, once acquired, MRSA can be long-lasting. A patient that gets MRSA during a hospital stay has increased risk for MRSA infections after they are released. MRSA carriers also have the potential to further transmission as they move through and across healthcare facilities. The quality of MRSA control in one facility may influence the MRSA experience in others. If MRSA is not controlled it can result in severe illness. For example, if it reaches your blood, lungs, or joints more serious illness can happen or even pneumonia. The Michigan Department of Community Health found that the number of MRSA report is rising in Michigan. In 2002 there were about 5,000 MRSA. By 2008 there were over 15,000 MRSA cases. There are still many ways to prevent MRSA though, and many of them are being practiced in hospitals and in schools. Some of these precautions include hand hygiene practices, implementing contact precautions, recognizing...
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...UTAR FHSC1214 Fundamentals of Cell Biology Trimester 1 How YOU can do well in BIOLOGY Follow the 4A’s and you can expect A’s. A ttitude • Attend ALL lectures, tutorials and practicals on time without fail. • Be attentive in class and revise your notes after class while the topic is still fresh in your mind. Why waste time re-reading 2-3 months later? • Do your assignments faithfully as they carry marks for the finals. • Come prepared for lessons (i.e. read up beforehand). • Read up beforehand before attending lectures so that you won’t be lost and wasted hours of your life week after week. • Why stress yourself out if you can avoid it? Do NOT count on last minute revision for tests and examinations, as it will be too late to catch up and seek help in areas where you may find confusing or unclear of. • Why panic before exams because you can’t find this or that? Keep separate files for lecture, tutorial and practical. File up the respective notes systematically so that you do not lose them along the semester. • Do you expect the lecturer/ tutor to be available all the time to answer your questions? It is YOUR responsibility to take the initiative to clear your doubts or satisfy your curiosity to understand certain scientific phenomena by reading up on the relevant topics. A Based on a true story… A professor at the National University of Singapore recounts how on one occasion a student consulted him days before the exam. Student:...
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...usually from a different species. For example, an artificial combination of genes that includes a gene to produce the pesticide Cry1Ab protein (commonly known as Bt toxin), originally found in Bacillus thuringiensis, is inserted in to the DNA of corn randomly. Both the location of the transferred gene sequence in the corn DNA and the consequences of the insertion differ with each insertion. The plant cells that have taken up the inserted gene are then grown in a lab using tissue culture and/or nutrient medium that allows them to develop into plants that are used to grow GM food crops.2 Natural breeding processes have been safely utilized for the past several thousand years. In contrast, "GE crop technology abrogates natural reproductive processes, selection occurs at the single cell level, the procedure is highly mutagenic and routinely breeches genera barriers, and the technique has only been used commercially for 10 years."3 Despite these differences, safety assessment of GM foods has been based on the idea of "substantial equivalence" such that "if a new food is found to be substantially equivalent in composition and nutritional characteristics to an existing food, it can be regarded as safe as the conventional food."4 However, several animal studies indicate serious health risks associated with GM food consumption including infertility, immune dysregulation, accelerated aging, dysregulation of genes associated with cholesterol synthesis,...
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...4. Protein Supplementation a. Protein supplementation b. Combining Protein Supplementation 5. Cellular Respiration & Effect on Weight Training a. Glycolysis b. Aerobic Respiration c. Anaerobic Respiration (inc. lactic acid) d. Carbohydrate Loading 6. Creatine Supplementation a. An Introduction b. Lab: Effect of Phosphocreatine on Lactic Acid 7. Anabolic-Androgenic Steroids a. Reactions within the Body involving steroids b. Side Effects of Steroid Intake c. Detecting Steroids in the Human Body 8. Conclusion 9. Works Cited 10. Miscellaneous Bodybuilding Page 2 of 59 I. Introduction Exercise (essentially any form of physical exertion which results in the contraction of a muscle) has become a widespread interest over the past several years, especially in areas of weight training. While exercise is generally intended to promote good physical health, bodybuilding more specifically concentrates on building muscle mass and many individuals in society today begin bodybuilding to present a good image of themselves. Many different companies have grasped on to this concept of muscle mass growth and have formulated products which can enhance the process of muscle enlargement. For example, creatine monohydrate, a product advertised to “boost muscle size and strength” and “improve athletic performance”, is available over the counter and has...
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...adding reliable references. Unsourced material may be challenged and removed. (January 2009) | Paclitaxel (Taxol) is a natural product derived from the Yew tree.[1] A natural product is a chemical compound or substance produced by a living organism - found in nature that usually has a pharmacological or biological activity for use in pharmaceutical drug discovery and drug design. A natural product can be considered as such even if it can be prepared by total synthesis. These small molecules provide the source or inspiration for the majority of FDA-approved agents and continue to be one of the major sources of inspiration for drug discovery. In particular, these compounds are important in the treatment of life-threatening conditions.[2] Contents[hide] * 1 Natural sources * 2 Screening of natural products * 2.1 The plant kingdom * 2.2 The microbial world * 2.3 The marine world * 2.4 Animal sources * 2.5 Venoms and toxins * 3 Traditional Medicine * 4 Isolation and purification * 5 Synthesis * 6 See also * 7 References * 8 External links * 9 Further reading | [edit] Natural sources Natural products may be extracted from tissues of terrestrial plants, marine organisms or microorganism fermentation broths. A crude (untreated) extract from any one of these sources typically contains novel, structurally diverse chemical compounds, which the natural environment is a rich source of. Chemical diversity in nature is based on biological and geographical...
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...[pic] MARKETING 201: PRINCIPLES OF MARKETING Instructor: Derick Davis, PhD Kosar/Epstein Faculty Office Wing, Office: KE-515 Phone Office: 305.284.6145 E-mail: ddavis@bus.miami.edu Section O: Tuesday and Thursday 9:30-10:45 Stubblefield Classroom 502 Section Q: Tuesday and Thursday 12:30-1:45 Dooley Memorial Classroom 200 Section R: Tuesday and Thursday 2:00-3:15 Dooley Memorial Classroom 200 Office hours: Tuesday and Thursday 3:30-5:00 Friday 9:00 – 11:00, or by appointment Please feel free to ask questions anytime. Email is the quickest way to communicate with me. However, it is unlikely I’ll respond after 8:00 P.M. REQUIRED MATERIAL A text will be used in class. The text is Marketing, by Grewal and Levy, 4th Edition, McGraw-Hill, 2013 is available in the bookstore (ISBN 978-0-07-802900-4) at a price of $154.00 for a new book. It is highly recommended that you read the assigned reading before the class in which it will be discussed. Additionally, chapter slides and/or outlines are available on the course website. It is recommended you review and print these, bringing them to class to help you with note-taking. We will be discussing three HBR cases. You have to download them from https://cb.hbsp.harvard.edu/cbmp/access/28012946 They will be used for individual assignments and also discussed in class. The cost is around $12.00. We may also use several articles throughout this course (available on Blackboard) under Course Reserves...
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...NUTRITION 121 HUMAN NUTRITION LABORATORY BACKGROUND AND METHODS MODULE #1 BODY COMPOSITION Late Labs: 10 point deduction! A report submitted >1 week late, will receive 0 points for the lab!! Written and edited by: Karon Felten and Nutrition 121 TA’s With Special Thanks to: Chris Pritsos Ph.D. Nutrition Department Chairman ABSTRACT: In nutrition, there are many factors that influence an individual’s health and body weight, such as heredity, food selection, eating behaviors, and physical activity. Scale weight is not always a good indicator of body fat, lean mass, body water or overall health (1). This laboratory experience will give students the opportunity to conduct physical assessments by evaluating body weight and body composition. To determine body weight and body composition, techniques such as bioelectrical impedance analysis (BIA), body mass index (BMI), waist circumference, wrist circumference for determining frame size, and a scale will be utilized. Students will also evaluate their own risk for cardiovascular disease and assess cardiovascular health in volunteers by measuring heart rate, filling out a heart disease risk score form, and estimating oxygen consumption and energy expenditure through a technique known as the Queens College Three-Minute Step Test. BACKGROUND: Your body is made up of the six essential nutrients: water, fat, protein, carbohydrates and various vitamins and minerals. Many factors can influence an individual’s...
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