Premium Essay

Infection Control Barrier Lab Report

Submitted By
Words 456
Pages 2
Objective The purpose of this lab was to demonstrate the effectiveness of infection control barriers in a lab setting and to review the fundamental skills from the Introductory Microbiology lab such as aseptic technique, streak for isolation, and microscopy. The materials used in this lab were: gloves, Tryptic Soy Agar (TSA) plates, a sterile loop, microscope slides, and Gram staining reagents (Crystal violet, Iodine, 95% Ethanol, and Safranin).The bacteria that was used was a culture of Serratia marcescens and a mixed broth culture of Escherichia coli and Staphylococcus epidermidis.
Discussion
After conducting the “Effectiveness of masks” experiment, the results from Figure 1 indicated that the mask was an effective barrier for a cough. According to Figure 1, the TSA plate on the right side (protected cough) did not display any form of bacteria growth after incubation. However, the TSA plate on the left side (unprotected cough) did not display bacterial growth either. The lack of bacterial growth from the TSA plate with the unprotected cough could be due to not having a vigorous cough. …show more content…
Based on Figure 2, each bench members’ handwashing technique was not effective. However, the two quadrants on the left showed less bacterial growth than the two quadrants on the right. Thus, the two quadrants on the left side hand washing technique were more effective than the two right quadrants. Overall, the ineffective handwashing technique results from this experiment may have been caused by the amount of soap that each person obtained and the amount of time spent on washing their

Similar Documents

Free Essay

Bacteria and Antibiotics Lab Report

...BACTERIA AND ANTIBIOTICS LAB REPORT Bryan Bennett Ms. Johnson Biology 09 period 8 March 12, 2013 Introduction: 1. Eubacteria -Has cell wall with peptidoglycan -They can live nearly anywhere on earth (sky to underground) -Unicellular -Prokaryotic -Reproduce Asexually Archaebacteria -Cell wall without peptidoglycan -Live in environments without oxygen (anaerobic) -Prokaryotic -Unicellular -Reproduce Asexually -Oldest bacterial form -Unique lipids in their cell membrane -DNA sequence is more like other Eukaryotes than other bacterial types (eubacteria) 2. Bacteria are classified into four groups: * PHOTOAUTOTROPHS * PHOTOHETEROTROPHS * CHEMOAUTOTROPHS * CHEMOHETEROTROPHS 3. 4. Many bacteria are heterotrophic which is to thrive off other organisms. The type of bacteria that causes disease are heterotrophic parasites. There are also many harmless bacterial parasites, many of which can be helpful to their hosts. Autotrophic bacteria manufacture their own food by chemosynthesis and photosynthesis. In aerobic respiration a series of reactions convert glucose to carbon dioxide and water and give off energy. Free oxygen is required as the final acceptor for electrons and hydrogen to form water. Bacteria, able to grow in the presence of oxygen, are called aerobic bacteria. Pseudomonas is an example of aerobic bacteria. In anaerobic respiration free oxygen isn’t required. Organic compounds are the final electron acceptors in anaerobic...

Words: 1569 - Pages: 7

Free Essay

Care Plan

...created to improve the mortality rate of patients who had colonic adenocarcinomas but is now indicated for | |several pathologies including complicated and severe diverticulitis, rectosigmoid cancer, and in cases where a colon resection is needed but a primary anastomosis cannot be safely done. There are few | |contraindications to the procedure and is often the procedure of choice when other complicated procedures cannot be performed. Patients with hypotension, renal failure, diabetes, malnutrition, immune | |compromise, and ascites can have unfavorable performance to the procedure. | |The important labs for this patient are the CBC(WBC,H&H, and diff), CMP, ABG if intubated still, lactic acid if still septic/possibly septic, if still on TPN (glucose, calcium, magnesium, phosphate, | |LFT’s, albumin)...

Words: 2513 - Pages: 11

Free Essay

Care Plan

...RACE/ETHNICITY: CULTURAL CONSIDERATIONS: Caucasian None RELIGION/SPIRITUAL CONSIDERATIONS: Unknown OCCUPATION/HOBBIES/RECREATIONAL ACTIVITIES: Retired LIVING SITUATION/WITH WHOM: (home, assisted living, LTC, etc) Lives with daughter. SOCIAL HISTORY: (tobacco, ETOH, illicit drugs, family dynamics) Quit smoking many years ago, no history of ETOH or drug use. NURSING CARE PLAN ADMITTING MEDICAL DIAGNOSIS: Client's principal admitting diagnosis was leukocytosis. Definition: (from Taber’s) “An increase in the number of leukocytes (usually above 10,000/mm3) in the blood. It occurs most commonly in disease processes involving infection, inflammation, trauma, or stress, but it also can result from the use of some medications” (Venes, 2009, p. 1327). Etiology/pathophysiology: ( NOT from Taber’s or Wikipedia) Etiology: Causes of leukocytosis are infection, inflammation, tissue damage, immune reaction, bone marrow problems, medications, and stress (Drug Information Online, 2011). Pathophysiology: “Leukocytosis can be a reaction to various infectious, inflammatory, and, in certain instances, physiologic processes (eg, stress, exercise). This reaction is mediated by several molecules, which are released or regulated in response to stimulatory events that include growth or survival factors (eg, granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, c-kit ligand), adhesion molecules (eg, CD11b/CD18), and various cytokines (eg, interleukin-1, interleukin-3...

Words: 3941 - Pages: 16

Premium Essay

Whooping Cough

...WHOOPING COUGH Pertussis/Whooping Cough Mary Beth Gibson Ashford University August 27, 2012 Instructor Kristin Akerele What is pertussis? What describe the epidemiology of pertussis? Who provides services to patients with pertussis? The Government mandates we have policies and procedures in place? Identify barriers that might make it difficult for treatment or patients to access services needed? Communication sometimes causes challenges for the public. In order to maintain our safety we should have strategies for communication to follow. The federal government makes sure the states and local levels are following protocol. What is pertussis, commonly known as “whooping cough”? Whooping cough is an infection of the respiratory system causes by bacterium bordetella pertussis. Pertussis is characterized by severe coughing spell, which makes a “whooping” sound when you breathe. Pertussis usually affects infants younger than 6 months old that are not adequately protected by immunizations. It also affects youth 11 to 18 years old, because their immunity is starting to fade. Some of the first symptoms of whooping cough are similar to common cold, such as runny nose, mild cough, and low grade fever and sneezing. “After about 1 to 2 weeks, the dry, irritating cough evolves into coughing spells. During a coughing spell, which can last for more than a minute, the child may turn red or purple. At the end of a spell, the child may make a characteristic whooping sound when breathing...

Words: 1378 - Pages: 6

Premium Essay

Abc Hospital

...Introduction: If we speak about this subject before 15 – 20 years back, the subject would be very unique and most of the people will have no understanding about this. But today, Quality tools understanding and importance has grown among people and organizations. There are many tools that are used among the organizations that are classified as follows: 1. Cause Analysis Tools: includes Fishbone, Pareto, and Scattered Diagram. 2. Evaluation and Decision Making Tool: Decision Matrix, Multivoting. 3. Process Analysis Tool: includes Flow chart, Failure Mode Effects Analysis, Mistake-proofing and Spaghetti Diagram. 4. Data Collection and Analysis Tools: box and whisker plot, check sheet, control chart, Design of experiments, Histogram, Scatter Diagram, Stratification, and Survey. 5. Idea Creation Tools: Affinity Diagram, Benchmarking, Brainstorming, Nominal Group Technique. 6. Project Planning and Implementation Tool: Gantt Chart, Plan-Do-Check-Act (PDCA) Cycle or Plan-Do-Study-Act (PDSA) Cycle. 7. Seven New Management and Planning Tools: Affinity Diagram, Relations Diagram, Tree Diagram, Matrix Diagram, Matrix Data Analysis, Arrow Diagram, Process Decision Program Chart. Quality tools & techniques use statistical knowledge to accumulate data and analyze them. It serves diverse range of medical, computing, industrial, telecommunications and defense. These tools drive improvement throughout the organization. Employee has to at all levels has to master the fundamental...

Words: 3917 - Pages: 16

Premium Essay

Pneumonia Case Studt

...morbidity and mortality is Pneumonia. Pneumonia is a lung infection caused by bacteria, fungi or viruses (Center for Disease Control, 2014).The infection is classified into two categories; community-acquired pneumonia (CAP) or health care-associated pneumonia (HCAP). The classification is determined by the environment in which the infection develops (CDC, 2014). HCAP develops during or following a stay in a healthcare facility. In contrast, patients who are diagnosed with CAP have had no contact within a health care setting prior to presenting with the infection (Driver, 2012). Pneumonia may be present as a mild illness but has the potential to be life-threatening. Despite advances in research, pneumonia remains a common illness contributing to the death of young children in developing countries and the elderly population of developed countries throughout the world (Ruuskanen, et al., 2011). In 2010, approximately 50,000 people in the U.S. died after developing the infection (CDC, 2014). From a global perspective, 450 million cases of pneumonia are recorded annually and roughly 4 million of those diagnosed will die from this illness (Ruuskanen, et al., 2011). CAP is the eighth-leading cause of death within the U.S. and is the leading cause of death from infection in the developed world (Brown, et al., 2012). While anyone is susceptible to contracting it, certain risk factors increase the chances of developing the infection. Research confirms that pneumonia is more serious in younger...

Words: 3087 - Pages: 13

Premium Essay

Task 4

...| Task 4 | | | Monica DeWitt | | | Current Compliance Status The hospital is compliant in with the National Patient Safety Goals (NPSG) in the following areas: staff is using 2 identifiers when providing care, correctly transfusing patients, maintaining a healthy patient care environment by complying with the Center for Disease Control (CDC) and World Health Organization (WHO) hand hygiene guidelines, continuing evidence-based best practice to prevent or reduce the risk of catheter-associated urinary tract infections (CAUTI), identifying patients at risk for suicide. Within the rest of the accreditation requirements the facility was compliant with the following elements: EM-emergency management HR-Human Resources IC-Infection Prevention and Control IM-Information Management MS-Medical Staff PI-Performance Improvement RI-Rights and Responsibilities of the Individual TS-Transplant Safety Trends of noncompliance within the healthcare system From the list of recorded finding there are several trends identified in which the hospital is will need to address to meet the Joint Commission (JC) standards. The list is divided into direct and indirect impact. There are 4 indirect impact issues that need to be addressed and 1 direct impact issue. Indirect Impact Trends Verbal Orders-Verbal orders are not being authenticated within the 48 hours on several units. The hospital audits should show a 100% compliance regarding verbal orders...

Words: 1189 - Pages: 5

Premium Essay

Nabh

...Date: 17/05/11 Page 2 of 53 NABH Assessment Checklist CONTENTS Clinical Areas S. No Department/Area Page Number 1. Emergency and Ambulance 5 2. Out Patient Department 6 3. Wards 7 4. Specialized wards 11 5. Palliative Care 12 6. Dialysis Unit 13 7. Intensive Care, Neonatal/ Paediatric ICU and High Dependency Units 14 8. Operation Theatre 17 9. Recovery Room 19 10. Endoscopy 20 11. Rehabilitation 22 12. Imaging: X Ray/ USG/ CT Scan/ MRI 22 13. Nuclear Medicine 24 14. Cardiac Catheterization lab 25 15. Laboratory: Haematology/ Microbiology 16. Blood Bank 27 17. Radiation therapy/Radioactive drugs 28 18. Nutrition 29 19. Research 30 20. Hospital Infection Control 30 Version 1 Biochemistry/ Issue Date: 17/05/11 Pathology/ 26 Page 3 of 53 NABH Assessment Checklist Non Clinical Areas S. No Department/Area Page Number 1. Document Review 32 2. Quality...

Words: 7807 - Pages: 32

Premium Essay

Cleaning vs Disinfecting

...2013-14 Advanced Environmental Technologies 2013-14 Holly Cross Athletic Facility Decontamination Analysis Cleaning vs. Disinfecting Introduction Cleaning and disinfecting of public transportation is a matter of technique, coupled with the proper cleaning agents. To insure that cross contamination of harmful microbes does not occur, certain steps need to be followed to take cleanliness down to the microscopic level, where the cause of the problem actually originates. Current practices of popular cleaners often instruct the user to spray on, wait 30 seconds, and wipe. They also do little to stop cross contamination as the user moves from one container to the next. For containers, airplanes, and intermodal vehicles that go all over the world, the simple act of “cleaning” with a less powerful agent could actually be the cause of a disease jumping from one country to the next. The current practices of using alcohol-based Simple Green, or similar products, instead of the more powerful chlorine dioxide could be a legal problem due to the to the lack of disinfecting properties required by insurance and governing policies. School buses play a major role in the intermodal world we live in. The public transportation network moves millions of people around the world in vehicles that are prime targets for the growth of microbials such as viruses, bacteria and fungi. About Microbes within Athletic Facilities Microbial populations of viruses, bacteria, and fungi are among...

Words: 1986 - Pages: 8

Free Essay

Micro Essay

...MicroBiology- MLT1 LabPaq / Published by: Hands-On Labs, Inc. sales@labpaq.com / www.LabPaq.com / Toll Free 866.206.0773 A Laboratory Manual of Small-Scale Experiments for the Independent Study of Microbiology 50-0222-MB-01 LabPaq® is a registered trademark of Hands-On Labs, Inc. (HOL). The LabPaq referenced in this manual is produced by Hands-On Labs, Inc. which holds and reserves all copyrights on the intellectual properties associated with the LabPaq’s unique design, assembly, and learning experiences. The laboratory manual included with a LabPaq is intended for the sole use by that LabPaq’s original purchaser and may not be reused without a LabPaq or by others without the specific written consent of HOL. No portion of any LabPaq manual’s materials may be reproduced, transmitted or distributed to others in any manner, nor may be downloaded to any public or privately shared systems or servers without the express written consent of HOL. No changes may be made in any LabPaq materials without the express written consent of HOL. HOL has invested years of research and development into these materials, reserves all rights related to them, and retains the right to impose substantial penalties for any misuse. Published by: Hands-On Labs, Inc. 3880 S. Windermere St. Englewood, CO 80110 Phone: Denver Area: 303-679-6252 Toll-free, Long-distance: 866-206-0773 www.LabPaq.com E-mail: info@LabPaq.com Printed...

Words: 31695 - Pages: 127

Premium Essay

Hiv in Homelessness

...HIV/AIDS & HOMELESSNESS Recommendations for Clinical Practice and Public Policy Developed for The Bureau of Primary Health Care and The HIV/AIDS Bureau Health Resources and Services Administration by John Song, M.D., M.P.H., M.A.T. November 1999 Financial and other support for the development and distribution of this paper were provided by the Bureau of Primary Health Care and the HIV/AIDS Bureau, Health Resources Services Administration, United States Department of Health and Human Services, to the National Health Care for the Homeless Council, Inc., and its subsidiary, the Health Care for the Homeless Clinicians’ Network. The views presented in this paper are those of the author and do not necessarily represent those of the United States government or of the National Health Care for the Homeless Council. Nothing in this paper should be construed as providing authoritative guidelines for the practice of medicine or for treatment of medical conditions. This paper may be reproduced in whole or in part with appropriate recognition to the author, John Y. Song, MD, and the publisher, the Health Care for the Homeless Clinicians’ Network, National Health Care for the Homeless Council, Inc. Second Printing February, 2000 National Health Care for the Homeless Council Health Care for the Homeless Clinicians’ Network Post Office Box 60427 Nashville TN 37206-0427 Phone 615/226-2292 Fax 615/226-1656 council@nhchc.org or network@nhchc.org http://www.nhchc.org i PREFACE HIV/AIDS...

Words: 29968 - Pages: 120

Premium Essay

Blood Work

...Essentials Ruth E. McCall, BS, MT (ASCP) Retired Program Director and Instructor Central New Mexico Community College Albuquerque, New Mexico President, NuHealth Educators, LLC Faculty, Emeritus Phoenix College Phoenix, Arizona Fifth Edition Cathee M. Tankersley, BS, MT (ASCP) Acquisitions Editor: Peter Sabatini Product Manager: Meredith L. Brittain Marketing Manager: Shauna Kelley Designer: Holly McLaughlin Production Services: Aptara, Inc. Fifth Edition Copyright © 2012, 2008 by Lippincott Williams & Wilkins, a Wolters Kluwer business. Two Commerce Square 2001 Market Street Philadelphia, PA 19103 351 West Camden Street Baltimore, MD 21201 Printed in China All rights reserved. This book is protected by copyright. No part of this book may be reproduced or transmitted in any form or by any means, including as photocopies or scanned-in or other electronic copies, or utilized by any information storage and retrieval system without written permission from the copyright owner, except for brief quotations embodied in critical articles and reviews. Materials appearing in this book prepared by individuals as part of their official duties as U.S. government employees are not covered by the above-mentioned copyright. To request permission, please contact Lippincott Williams & Wilkins at Two Commerce Square, 2001 Market Street, Philadelphia, PA 19103, via email at permissions@lww.com, or via website at lww.com (products and services). 9 8 7 6 5 4 3 2 1 Library of Congress...

Words: 129902 - Pages: 520

Premium Essay

Botulism

...management/ preparedness needed in order to effectively treat this disease. Botulism is a rare, naturally occurring disease that may be caused by deliberate or accidental exposure to the toxins of Clostridium botulinum. The three types of naturally occurring disease are food-borne, wound and intestinal colonization botulism, dependent on the route of ingress of the toxins. It may also be a result of biological terrorism. It presents with an afebrile, descending, symmetrical, flaccid paralysis of motor and autonomic but not sensory nerves. Respiratory failure can occur rapidly with little prior ventilatory deterioration. Management includes respiratory support, specific antitoxin and if wound related, surgical debridement and antibiotics. I will report different cases of botulism and discuss the presenting features that should alert the emergency physician to the diagnosis and direction of further treatment. ASSESSMENT AND SYNTHESIS OF THE PROBLEM Pathology The rare toxin is a protein produced under anaerobic conditions by the bacterium Clostridium botulinum, and affects a wide range of animals, including mammals, birds and fish. Clostridium botulinum is a large anaerobic Gram-positive bacillus that forms sub-terminal endospores. There are seven serological varieties of the bacterium denoted by the letters A to G. The toxin from all of these acts in the same way and produces similar symptoms: the motor nerve endings are prevented from releasing acetylcholine, causing flaccid paralysis...

Words: 4392 - Pages: 18

Premium Essay

Accrediation Audit

...Executive Summary Accreditation Audit- Task 1 Maggie Miklos January 25, 2014 Executive Summary At Nightingale Community Hospital (NCH) one of our core values is to provide superior service and outstanding clinical care as noted in our safety statement. We welcome The Joint Commission (TJC) to survey our facility on a triennial basis to ensure compliance with their established standards and Priority Focus Areas: Infection control, Communication, Medication Management, and Information Management. In anticipation of the unannounced site visit, I have reviewed our current state of compliance for gaps in the Communication focus area and have prepared actions to close the gaps to ensure compliance. To gauge NGH’s compliance in the Priority Focus Area, Communication, I have reviewed the following Universal Protocol Standards depicted in TJC Handbook. This protocol “…was created to address the continuing occurrence of wrong site, wrong procedure and wrong person surgery and other procedures in Joint Commission accredited organizations” (Joint Commission, 2013). Within each standard our facility must meet the Elements of Performance criteria. The three standards I will be reviewing are: UP.01.01.01: Conduct a preprocedure verification process. (Commission 2013) UP.01.02.01: Mark the procedure site. (Commission 2013) UP.01.03.0: A Time-out is performed before the procedure. (Commissions 2013) The rationale...

Words: 1510 - Pages: 7

Premium Essay

Miss

...Content Frame Skip Breadcrumb Navigation Home arrow Chapter 2 arrow Nursing Care Plan Nursing Care Plan Nursing Diagnosis Impaired Verbal Communication r/t sedation, presence of artificial airway, or decreased level of consciousness Long Term Goal: Patient is able to use a form of communication to get needs met and relate to his environment Short Term Goals / Outcomes: Patient and nurse will establish a means of communication Patient will be able to effectively communicate and needs Intervention Rationale Evaluation Assess the patient’s primary and preferred means of communication (verbal, written, gestures) Communication can be frustrating for both the nurse and patient. It is critical that the nurse and patient determine the best method for each patient. Patient can write words clearly on paper Assess the patient’s preferred language and ability to understand written words, pictures and gestures The nurse can not assume that the patient is grasping the information that is provided. In recognition of the vast array of cultures and physical challenges that patient’s face, it is the nurse’s responsibility to communicate effectively Patient speaks and reads English. Recognize that the presence of an artificial airway will hinder the patient’s ability to communicate When air does not pass over the vocal cords, sounds are not produced. Other methods of communication will have...

Words: 3558 - Pages: 15