Title: To investigate the effect of temperature on the rate of respiration.

Introduction: In this experiment, we are going to investigate how the rate of respiration is affected by changes of temperature.

Hypothesis: I think that the rate of respiration increases as the temperature increases; on the contrary, the rate of respiration decreases as temperature decreases. The reason is that as temperature increases until a certain point, acetyl-CoA works most efficiently, so that the amount of O2 and Co2 will increase as well. For instance, body temperature maintains constantly around 37℃. At this temperature, human beings can work more efficient than other extreme high or extreme low temperature.

Experimental Design:

Independent variable:
Different temperatures (Inside & Outside)

Dependent variable:
The rate of respiration (Change in O2 & Change in Co2)

Controls: * Timer * Pressure * Type + Number of worms * Volume of container * The initial amount of O2 and Co2

Materials: * 8 worms * 2 O2 sensors * 2 Co2 sensors * 2 Biochampers (250 ml) * 2 Thermometers * 2 Timers * 2 Lab Quest Minis * 2 Laptops

Risk Assessment:

Procedure:
Put 4 worms in a biochamper inside the classroom.
Set up O2 sensor and Co2 sensor in the biochamper.
Set up O2 sensor, Co2 sensor and thermometer on the Lab Quest Mini, and then on the laptop.
Wait for 2-3 minutes for Co2 inside to be even.
Start the timer for 40 minutes and record the data.
Put other 4 worms in another biochamper outside the classroom.
Set up O2 sensor and Co2 sensor in the biochamper.
Set up O2 sensor, Co2 sensor and thermometer on the Lab Quest Mini, and then on the laptop.
Wait for 2-3 minutes for Co2 inside to be even.
Start the timer for 40 minutes and record the data.

Results & Data:

Temperature (℃) | Amount of