...similarities to study individual macromolecular components found within all living cells. Through this experiment we will learn the basic sub-units that make up each of these macromolecules while also learning some of their important structural characteristics. This experiment will consist of two parts. The first of which will divide the yeast cells into three of its major macromolecular components: nucleic acids, proteins and polysaccharides. These components are large macromolecules that are quite unique in their composition, structure and function. However, they share a common feature as each macromolecule is composed of repeating subunits, characteristic of the macromolecule. The subunits are linked together by a bond between two adjacent subunits, formed by the loss of water (condensation). Thus, macromolecules can be broken down by the addition of water across the bond, in a process known as hydrolysis. This process was used in the experimental procedure to allow analysis of each individual macromolecule in its subunit form. Proteins are hydrolyzed into amino acids, nucleic acids are hydrolyzed into sugar, base and phosphate, and polysaccharides are broken down into simple sugars. In the second part of the experiment, the principle method of chromatography was used to analyze the macromolecules isolated in part one of the experiment. With this technique, individual molecular species were separated from one another. This separation technique was performed for the protein and...
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...formaldehyde (H2CO), hydrogen cyanide (HCN) 2. Formation of mid-sized molecules from these small compounds * i.e., amino acids, simple sugars 3. Mid-sized building blocks combine to form large molecules. * i.e., proteins, complex carbohydrates 4. Life became possible when one of these large molecules self-replicated. * Organic Molecules * Large organic molecules are called macromolecules * Four major categories of macromolecules: 1. Proteins 2. Nucleic Acids 3. Carbohydrates 4. Lipids * Several of these are long chains of smaller subunits. * The smaller subunits are known as monomers * The long chains of monomers are known as polymers * Four groups of Macromolecules * Building Macromolecules – Monomers and Polymers * Molecules, such as amino acids, are individual units called monomers. They link together (polymerize) to form polymers, such as proteins. * Macromolecules are very large polymers made up of many monomers linked together. * Thus, proteins are macromolecules consisting of linked amino acid monomers. * Energy is required from the cells to build molecule * Assembling and Breaking Apart Polymers: Condensation and Hydrolysis Reactions * Monomers polymerize to form polymers. * Polymerization reactions...
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...you find proteins inside cells or cells inside proteins? (choose one answer) 4. What two things make osmosis a special type of diffusion? 5. What is a selectively permeable membrane? (Also called a semi-permeable membrane.) 6. Explain what happens to a cell when placed in isotonic, hypotonic and hypertonic solutions. 7. Explain the basic organization (main components) of a plasma membrane. Include at least 4 distinct parts. 8. Identify (a) a similarity and (b) a difference between facilitated transport and active transport; do not state they both move substances across membranes. 9. What is an organelle? Which organelle is a processing, packaging and shipping center for proteins and lipids? 10. Where would you find the nucleolus? What happens there? 11. What is the role of the ribosome? 12. Which organelle digests macromolecules, worn out cell parts, debris and disease-causing microbes? 13. What structures are associated with cell movement? 14. What is the most significant difference in structure between rough and smooth ER? What type of macromolecule do each produce? 15. Which organelle makes ATP? 16. What is the most important product of cellular respiration? 17. List the three major steps in cellular respiration. (3 pts) 18. How does the body use the oxygen that we breathe? In other words, what is oxygen's final function? 19. What ATP producing process occurs during anaerobic conditions? 20. Glucose enters cells from the blood stream by what type of transport...
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...Objectives : 1. To test for the present of carbohydrate in food samples by using Benedict’s reagent. 2. To determine the present of starch in food samples by using iodine reagent. 3. To investigate the present of protein in food samples with Biuret solution. 4. To determine the present of lipid in food samples using brown paper. Introduction : We had learned about that all living organisms are made up of molecules, each molecule serve its own special purpose. Molecule can be divided into two types, macromolecules and micro molecules. The molecules that we are studying in this experiment are macromolecules. In this case, we are studying on 3 types of macromolecules, carbohydrates, protein and lipid that present in different food. Carbohydrate can be divided into monosaccharide, disaccharide and polysaccharide. Monosaccharide is the simplest sugar that appears in food, the most common example of this is glucose. Disaccharide is a double sugar, which means by it linked two monosaccharides together to form a disaccharide. The examples for disaccharide are maltose, galactose and sucrose. The example of polysaccharide is starch. Protein is build up by the monomers, amino acid. Each protein has it own special sequence that is different from others. The Biuret reagent is used to determine the presence of protein as it turns the original blue colour of the solution to purple when it react with the protein, specifically between the bonds of amino acid. Lipids are...
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...The purpose of this lab was to use different indicators to determine what macromolecules are in different foods. We used Lugol’s solution to determine the starch content, Benedict’s solution to see the sugar content, Biuret reagent to test for the proteins, and used a paper bag to determine the lipid content. Materials: Vegetable Oil Glucose Starch from corn or potatoes Powdered egg whites Water Lugol’s solution Benedict’s solution Biuret reagent Pretzel Toast Jelly Pineapple Fat-free yogurt Beans Salami Swiss cheese Test Tubes Test Tube Holder Beaker Hot plate Analysis #1 1. Does dH2O contain any macromolecules? Why did we test dH2O with each of the indicators? DH2O does not contain any macromolecules. We tested dH2O with each of the indicators because we needed a base to make sure the solutions were accurate in testing the controls. 2....
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...MCDB1A Lecture Schedule, Fall 2013 Part 1: Introduction to Biochemistry and Molecular Biology Dr. Stuart Feinstein Email: stu.feinstein@lifesci.ucsb.edu Office: Bio II, Rm. 5123; Office Hours: T and F after Lecture, in front of Campbell Hall and by appointment (after Oct. 22, T at 9 AM in Bio II, Rm. 5123). Approximate Lecture Schedule for Biochemistry/Molecular Biology Date | TOPIC | Reading in Text*[chapter (pages)] | | | | 9/27 | Introduction to class and Biochemical Principles | 1 (1-20); 2 (21-37) | | | | 9/30 | Introduction to Macromolecules/ Lipids and Polysaccharides | 3 (39-42; 51-61);6 (105-109) | | | | 10/1 | Macromolecules: Polysaccharides and Proteins | 3 (42-51) | | | | 10/2 | Macromolecules: Proteins and Nucleic Acids | 4 (62-67);13 (259-280) | | | | 10/4 | Macromolecules: Nucleic acids as Genetic Material | 13 (259-280) | | | | 10/7 | The Central Dogma: DNA Replication | 13 (259-280) | | | | 10/8 | The Central Dogma: Transcription and Translation | 14 (281-303) | | | | 10/9 | The Central Dogma: Translation and Mutations | 14 (281-303)15 (304-308) | 10/10 | First Honors Meeting | | 10/11 | The Central Dogma: Post-Translational Modifications, Alternative RNA Splicing and MicroRNAs | 14 (300-304;312-313) 16 (346-349) | | | | 10/14 | Energy, Enzymes and Metabolism | 8 (144-164) | | | | 10/15 | Chemical Pathways that Harvest Chemical Energy | 9 (165-184)...
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...practice exercises, students should be able to: • Define the biologically relevant interactions (bonds) between molecules • List functional groups commonly found in biological molecules. • List the different kinds of biological macromolecules and their monomers. • Summarize how polymers are made and broken down and how water participates in these reactions. • Describe the structure and functions of: o Simple sugars. o Nucleotides o Amino acids • Summarize the different forms and functions of complex carbohydrates. • Describe the possible levels of protein structure. • Summarize the fundamental property of all lipids • Illustrate the structure of triglycerides and phospholipids. Advanced learning objectives After the biomolecules lectures, students should be able to: • Predict what type of bond would be formed using electronegativity information. • Develop the critical thinking skills that allow you to evaluate scientific experiments that seek to explore how life started on earth. • Summarize why and where carbohydrates are commonly combined with other macromolecules. • Describe the relationship between functional groups and the molecules they are part of. • Compare and contrast the structure and functions of DNA and RNA. • Describe the four types of lipids. o Summarize and predict commonalities and differences in lipid structure and function • Describe how man-made...
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...different macromolecules by using the biochemical tests. This lab also indicates the hydrolysis of macromolecules when heat or acidity is applied to the molecules or compounds. The molecules that are being observed in the experiment are the polysaccharides and proteins, two main components that play an important role in cell biology. Hydrolysis could be done by applying heat, acid treatments, enzymic reaction, and bacteria involvement. IKI test and Benedict’s test is used in the experiment in order to detect the type of carbohydrate present in the testing tube containing the testing sample. In this experiment Hydrolysis is done through different methods. The first method is the hydrolysis of polysaccharides by adding acid or heat to the experiment. Starch is the polysaccharide being used in the experiment that is diluted with water. Starch solution, water, and glucose are tested for the presence of polysaccharides in it. The results are recorded in “before treatment” section. Same process is repeated for the benedict’s test that includes slight boiling, for the indication of reducing sugars. HCl is added to the test tubes that are placed into the bath tub at boiling temperature. The results are recorded in “after treatment” sections for both IKI and Benedict’s test. Color change is observed in the starch after adding heat. Results indicate that change in heat and acidity affect the hydrolysis. Glucose is a monomer while starch is a polysaccharide. In the second part of the experiment...
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...birth, hatching, or germination.¨Germination is the process in which plants grow. Comparative Anatomy is the body structures in different species and how they are similar. In 1555 a man named Pierre Belon showed that human skeletons and and bird skeletons are somewhat the same. He showed that our skeletons were the same elements of a birds just constructed a different way. Comparative Anatomy is evidence to prove that evolution is real. We have a lot of similarities with monkeys. Similarities such as our hands, fingers, toes, ears, etc. Molecular Biology is the study of biology but it explores the cell and pays a lot of attention to how molecules control the cell's activities. It is also the part of biology that deals with the structure and function of macromolecules. Macromolecules such as proteins and nucleic acids. It seeks to explain the phenomena of life. Molecular Biology seeks to explain the phenomena of life through the study of molecules found in living things. A biography is a story of someone's life written by someone else. Direct observation is a method that you use when you are collecting evaluative information. When you put the two together you get a story written about someone that was using the direct observation when trying to find evidence to support why evolution is real. When observing you watch the subject or subjects without altering the environment. All of these subjects relate and come down to one thing. To pull this all together all these topics...
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...The isolation and purification of genomic DNA requires three main processes which include, lysing of cells to release the DNA, purifying the solution by removing unwanted macromolecules, and precipitating the DNA from the solution. The process of isolation and purification requires several reagents. The Nuclei Lysis Solution contains lysozyme and EDTA. The lysozyme is an enzyme that degrades peptidoglycan, which is a rigid exoskeleton cell wall that protect the bacteria (3). Disrupting the peptidoglycan will result the release of the content inside the cell and the death of the cell. In addition to inhibiting DNases, the EDTA in the lysis solution aids the lysozyme to access the peptidoglycan by removing the Mg2+ from the lipopolysaccharide...
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...EUSTINE THULAMBO CONCEPTS OF NUTRITIONAL HEALTH P2 A balanced diet means eating a variety of foods in the right proportion, and consuming the right amount of food and drink to achieve and maintain a healthy body weight. A balanced diet consists of vitamins, carbohydrates, minerals, vitamins, fats (lipids) and fibre which is also known as roughage. CARBOHYDRATES Carbohydrates are macromolecules which and contains carbon, hydrogen and oxygen (C,H,O). There are also two types of sugars, sweet soluble and starches which are non-soluble. Sugars can also be single or simple sugars.Carbohydrates can be found in a wide variety of foods such as pasta, wheat and grains, rice, potatoes, fruits and sugars. Carbohydrates are broken down into energy by the digestive system, in the form glucose. Carbohydrates provide energy in our bodies needed for various purposes such as the synthesis of biochemical macromolecules, transportation and muscle concentration. PROTEINS Protein is the major structural component of cell which is made up of several elements which are carbon, hydrogen, oxygen, and nitrogen(C, H, O, N). They are essential for growth, repair and maintenance of body tissues. There are animal and non-animal food sources which are rich in proteins such as meat, fish eggs beans and lentils. When consumed, proteins are broken down by digestive system into amino acids which are sometimes referred to as building blocks of proteins. Proteins consist of a long chain of amino acids...
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...INTRODUCTION Carbon, hydrogen, oxygen, nitrogen, sulfur and phosphorus are a very important part of all living organisms. These elements combine is various ways to make the molecules that make up biological systems. There are four major molecules (macromolecule) found in living organisms – Carbohydrates, Lipid, Proteins and Nucleic Acids. They all have different chemical properties and can be identified by their functional groups. A chemical test that is sensitive to these functional groups can be used to identify or test for the presence of each of the macromolecules. This lab tests for major macromolecules using such tests. (Karp, 2005) The Iodine test is used to detect starch and glycogen, the Benedict’s test is used to test for reducing sugars and the Biuret test is used to detect protein. (Landesberg, 2011) The Iodine test is useful to distinguish starch and glycogen from other polysaccharides. A polysaccharide is a carbohydrate whose molecules consist of sugar molecules. Plants and animals store their simple sugars in the form of polysaccharides. The polysaccharide that plants use is called starch, and it has a lot of glucose units connected together with glycosidic bonds. Starch also has a polymer called amylose, which reacts with the iodine during the test and gives it a blue-black colour. Glycogen, in animals, is bigger (in terms of molecular weight) and it’s structurally very different. It has a lot of branches and they give glycogen the reddish-brown colour in...
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...movement of molecules in and out of the nucleus (through nuclear pores). When the chromosomes are not diving they are in the form of chromatin, the nucleus contains the chromatin. It contains DNA. There are two forms of chromatin: euchromatin and heterochromatin. Euchromatin contains inactive DNA and heterochromatin inactive. Michael Kent (2000) explains ‘the nucleus also contains one of more nucleoli. These manufacture ribosomes’. In the nuclear envelope in a substance called nucleoplasm or karyoplasm and it similar to cytoplasm. It is mainly water, enzymes and dissolves salts. The nucleus has a nucleolus which is membrane-less and is made of RNA and proteins. Thoughtco (2017) explains ‘The nucleolus contains nucleolar organizers, which are parts of chromosomes with the genes for ribosome synthesis on them’. Thoughtco further explains how the nucleolus helps to ‘synthesize ribosomes by transcribing and assembling ribosomal RNA subunits’. In protein synthesis, sub-units will join and become a ribosome. Peroxisome Peroxisomes are small organelles, that are membrane bound and are cytoplasmic. They are found in both animal cells and plant cells. They are responsible for a lot of metabolic activities, breaking down fatty acids and amino acids. In some cells they are responsible for lipid break-down. The enzymes produce a biproduct, hydrogen peroxide, this can be dangerous for the cells so peroxisomes will isolate it. An enzyme called catalase is able to break down hydrogen peroxide. Peroxisomes...
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...In this lab, we were trying to identify certain macromolecules commonly found in food and other substances. These macromolecules were carbohydrates(both simple and complex), proteins, and lipids. Carbohydrates are a simple, short-term source of energy, and they are used in making cell structures. Carbohydrates are made up of monomers(which are comprised of single molecules) called monosaccharides. Monosaccharides include glucose, sucrose, and galactose. Two monosaccharides can be combined by removing a hydrogen end from one monomer and an oxygen/hydrogen end from another(forming a water molecule), and they can be combined using a bond called a glycosidic bond. These are called disaccharides, and can include carbohydrates like sucrose. When three or more monosaccharides are bonded together(once again using glycosidic bonds and...
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...function. As the nuclear envelope isolates and protects a cell’s DNA from various molecules that might damage the structure or its processing this when DNA is synthesized special RNA (Parker and Honeysett, biology, 2008). The nucleolus produces ribosome’s, which move out of nucleus to a position on the rough endoplasmic reticulum where they are critical in protein synthesis. Endoplasmic Reticulum which has a system of complex tunnels that spread throughout the cell, this part of the cell which is suspended. This helps food to be consumed and transform into energy for the cell functions. It has elaborate system which is called endoplasmic reticulum (Rowland, as biology, 2008); this is interconnected with the lining membrane with nuclear envelope. This given to the fact that is referred to as called cytosol, mean cell substance which is a grey colour when looked at it. As it is made up of two parts the ectoplasm, a dense gelatinous outer layer concerned with cell movement and endoplasm which has a more fluid inner part where most of organelles are found, as there are chemical reactions happening. The Endoplasmic Reticulum helps in the transport of proteins and lipids, as it covers over half a cell....
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