Free Essay

Microbiology an Introduction

In:

Submitted By vhremington
Words 708
Pages 3
Microorganisms are all round us. Everyday at any given minute our bodies contain flora or normal microbiota. Which as described in the textbook Microbiology An Introduction as, “Some normal microbiota protect us against disease by preventing the overgrowth of harmful microbes, and others produce useful substances such as vitamin K and some B vitamins. Unfortunately, under some circumstances normal microbiota can make us sick or infect people we contact. For instance, when some microbiota leave their habitat, they can cause disease. ” (Tortora, Funke, and Case 17) In my experience of working in a hospital on the medical-surgical floor, I have become very familiar with the opportunist infection formally know as Clostridium Diffcile and informally known as C. diff. C. Diff is an infection that affects the colon and causes the patient to have irretractable diarrhea and can even cause inflammation of the colon that can sometimes be lethal. C. diff is very common in the elderly and patients on multiple antibiotics which causes the natural bacteria in the colon to be irradiated allowing opportunistic bacteria to invade. The New York Times reported that “Two factors typically lead to the infection: taking antibiotics, which make the intestine vulnerable, followed by exposure to the bacteria or their spores in a hospital, clinic or nursing home that has not been properly disinfected. Spores can survive for weeks or maybe even months outside the body, and it takes bleach or other strong disinfectants to kill them. Many people recover once they stop taking antibiotics, but some become severely ill and require treatment with different antibiotics to get rid of C. difficile. Even then, 20 percent relapse, and some suffer intestinal damage that can be fatal or can require surgery to remove part of the intestine.” (Grady) C. diff is really difficult to treat because the bacteria is resistant to many antibiotics. The New York times also described an unconventional treatment, which is not commonly performed called a fecal transplant, “In recent years, some people have received fecal transplants, in which stool from a healthy person is placed into the patient’s intestine. The idea is to restore the normal balance of bacteria in the gut, which may include 25,000 to 30,000 different species. There is simply no better way to replace them. Awful as they may sound, the transplants can cure cases that were otherwise intractable.” (Grady) The premise of this treatment is to reintroduce good bacteria into the colon thus disallowing the C. Diff to replicate and take over. Hospital and physicians are taking extra steps in preventing the high incidents of C. Diff by prescribing scheduled probiotics, especially if they are taking antibiotics. A study conducted by Annal of Internal Medicine and featured in the NY Times researched the effectiveness of probiotics preventing the incidence of C diff. The study was used data from 20 randomized and controlled trials that compared a course of probiotics with a placebo. The study also used different types of probiotics with varying doses. The researchers were able to deduce from trails that compared to the subjects using the actual probiotics compared the placebo had a lower incident in diarrhea. The researchers ultimately found in their studies that probiotics reduced the risk of C. diff associated diarrhea by 66 percent. (Bakalar)

It is important to remember that your colon contains nature flora and when that flora is depleted by antibiotics it can put you at risk for opportunist infections such as C. diff and measures such be taken to reintroduce new bacteria in the colon and that can be done simply by taking a probiotic. With preventive medicine on the for front of healthcare it is important to remember some of taking steps to inhibit diseases like C. diff with simple measures like proper sanitization and most importantly washing your hands.

Works Cited

Bakalar, Nicholas. "Probiotics Linked to Lowered Diarrhea Risk." Well Probiotics Linked to Lowered Diarrhea Risk Comments. New York Times, 19 Nov. 2012. Web. 22 Jan. 2014.

Grady, Denise. "Gut Infections Are Growing More Lethal." Www.nytimes.com. New York Times, 19 Mar. 2012. Web. 22 Feb. 2014.

Tortora, Gerard J., Berdell R. Funke, and Christine L. Case. Microbiology: An Introduction. Boston, MA: Pearson Learning Solutions, 2012. 16-17. Print.

Similar Documents

Premium Essay

Unknown Microbes

...Microbiology is requires not only an academic understanding of the microscopic world but also a practical understanding of lab techniques and procedures used to identify, control, and manipulate microorganisms. The proper identification of a microorganism is not only important in a microbiology lab but also in the medical, industrial, and pharmaceutical fields. In this lab report, lab techniques and procedures learned during this course were performed to assess each students’ practical knowledge in microbiology. 6In area of fields I mention earlier microbiology is very important to our vaccination and antibiotics we are using, understand that microorganism play a key role in maintaining life on earth, fixing gases and breaking down dead plant and animal matter into simpler substances that are used at the beginning of the food chain 6. Biotechnologists can also exploit the activities of microbes to benefit humans, such as in the production of medicines, enzymes and food. The goal of this lab report is 1) to demonstrate comprehension of the methods and lab techniques learned during the semester 2) to explain the tests performed on each isolated unknown that led to the identification of each unknown 3) and to give a background on the characteristics, pathogenicity and some uses of one of the identified unknowns. II. Introduction In this lab report I will discuss how I came to find my two unknown bacteria. Each bacteria have undergo many different test to eventually identifying...

Words: 464 - Pages: 2

Free Essay

Reflective Report

...university and have no regrets. However at the beginning I did not know what I wanted to specialise in and what path I was going to take. It all changed once I begin studying the introduction to biomedical science, as it made me understand more about each profession and how everyday life working in a specific area of biology is like. Introduction to biomedical science has most definitely changed my mind on what career I want to do once I graduate. It has given me a wide outlook of the many different directions I can go with this course and explained to me what each of them contains. Overall this module has impacted my perspective of biomedical science. By doing this course I now am confident in what profession I want to specialise. It has taught me the importance of microbiology and how much of a big role it plays in healthcare and that without the study of microbes there would be no way for cures to be found of certain infections/diseases. From doing the patient case studies it has really taught me how scientists who specialize in different biology subjects come together to diagnose a patient. For example this showed me how a haematologist, microbiologist and a clinical biochemist work together to diagnose a patient of a certain infection/disease. In addition to this I did some more research into microbiology and have decided that this is the route I am going to specialise in in the future. This module has taught me how much biomedical scientists are a part of a patient’s wellbeing...

Words: 543 - Pages: 3

Premium Essay

Identification of Unknown Organism

...coli November 12, 2014 Dr. Brenda Deming Microbiology 2420 Fall 2014 Section 01 Introduction Microorganisms, which play an important role in the ecology of life, can be both beneficial as in the decomposition of waste products and harmful in the way that they can cause illness, diseases and even death in animals and humans. The purpose of this study was to identify two unknown organisms from a single mixed culture broth by using biochemical tests and procedures learned in the microbiology laboratory and the Biology 2420 Laboratory Manual for Microbiology by Mary Hearron, Ed. D. and Brenda Deming, Ph. D., M, T. (ASCP) (1). Materials and Methods On November 5, 2014, unknown #13, a mixed broth containing two unknown organisms was obtained from an arrangement set up by Dr. Deming. Each student chose an unknown from the arrangement and was not assigned a particular number to ensure that the project was blind and not rigged in any way. From that point, streak plates and biochemical testing was conducted to determine, the A and B unknowns. All media and biochemical tests were labeled with the name date, time, unknown A or B, and the test being conducted to eliminate confusion and errors. All procedures used unless otherwise noted were taken directly from the Biology 2420 Laboratory Manual for Microbiology by Mary Hearron, Ed. D. and Brenda Deming, Ph. D., M, T (ASCP) (1) and verified with A Photographic Atlas for the Microbiology Laboratory, 4th Edition by Michael J. Leboffe...

Words: 368 - Pages: 2

Free Essay

Microbiology

...HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased, not the writing. The purpose of scientific writing is not to entertain, but to inform. The writing should be simple and easy to understand. There is a specific style that must be followed when writing scientific reports. Scientific writing is typically written in the passive voice. The pronouns "I", "We" and "They" are not typically used.. For example, instead of writing "I used a TSA agar plate to isolate my unknown," it is customary to write, "A trypticase soy agar (TSA) plate was used to isolate the unknown." It is also customary to write in the past tense for most of the report. This includes the introduction, the summary, the description of the materials and methods and the results. The present tense is reserved for the conclusions about the results. See the examples given below. Some other general rules that should be followed are: Microbial nomenclature: The name of the bacterium should written and spelled correctly. The name should be italicized or underlined. Italicized is preferred. For example, Staphylococcus aureus. The genus is capitalized but the species is not. After the full genus name is given in the paper, it can be written as S. aureus, but still italicized. This is as long as there in no other...

Words: 1646 - Pages: 7

Premium Essay

Unknown Identification

...Unknown Identification Introduction to Microbiology Lab Professor M Biology Lab 1913 11/11/2014 I. Introduction A series of tests was completed in order to identify an unknown microorganism. A gram stain reaction test was done. A gram stain reaction test is used to differentiate between two bacterial species. The two species; gram positive and gram negative bacteria have varying properties of their cell wall structural composition. The gram positive bacteria contain a thick peptidoglycan layer in their cell wall which retains the primary crystal violet stain. The crystal violet is washed from the fixed stain and gram-negative bacteria appear red after the decolorizer washes the primary stain due to their more porous higher lipid content walls and the safranin counter stain adheres to their thinner cell wall. The microscopic examination of the bacteria after staining allows for the morphology of the organism to be determined because as the cell is killed during the staining process it retains its rigid structure allowing for morphology determination. A fermentation test was done. Three different carbohydrates are used to determine whether the organism can ferment a sugar as well as if a gas is produced during heterofermentation. A phenol red broth is used which retains a red color at a pH of 7.4 indicating no fermentation of a sugar. When an acid is produced during fermentation, the pH of the broth will lower and the broth will turn yellow. The sugars...

Words: 1675 - Pages: 7

Free Essay

Registered General Nursing Student

...Section A: Basic Microbiology 1 SCOPE AND HISTORICAL DEVELOPMENTS IN MICROBIOLOGY “Science contributes to our culture in many ways, as a creative intellectual activity in its own right, as a light which has served to illuminate man’s place in the uni-verse, and as the source of understanding of man’s own nature” —John F. Kennedy (1917–63) The President of America The bacterium Escherichia coli INTRODUCTION AND SCOPE MICROBIOLOGY is a specialized area of biology (Gr. bios-life+ logos-to study) that concerns with the study of microbes ordinarily too small to be seen without magnification. Microorganisms are microscopic (Gr. mikros-small+ scopein-to see) and independently living cells that, like humans, live in communities. Microorganisms include a large and diverse group of microscopic organisms that exist as single cell or cell clusters (e.g., bacteria, archaea, fungi, algae, protozoa and helminths) and the viruses, which are microscopic but not cellular. While bacteria and archaea are classed as prokaryotes (Gr. pro-before+ karyon-nucleus) the fungi, algae, protozoa and helminths are eukaryotes (Gr. eu-true or good+ karyon-nucleus). Microorganisms are present everywhere on earth, which includes humans, animals, plants and other living creatures, soil,water and atmosphere. Microorganisms are relevant to all of our lives in a multitude of ways. Sometimes, the influence of microorganisms on human life is beneficial, whereas at other times, it is detrimental. For example...

Words: 9515 - Pages: 39

Free Essay

Nitrate Reduction Test

...Nitrate and Nitrite Reduction Test Protocols HISTORY Current tests for nitrate and nitrite reduction are based on the Griess diazotization reaction described in 1858 by Peter Griess. Griess was raised on a farm in Prussia and was the son of a blacksmith, but “…tilling the soil was little to his liking, and on more than one occasion his father found him in a corner of the field, deep in a book, seated on the plough.” (25) In his early attempts at higher education, he was far from a model student, spending time in the institution’s prison and eventually expelled for a year. Finally, in his 6th year at university he began to seriously study chemistry. He obtained employment in the coal-tar distillery where the senior chemists discovered and developed the aniline dye industry. Even though the distillery was soon destroyed by fire, Griess had become obsessed with the chemistry of dye making. He was recommended for a position at the Royal College of Chemistry in Great Britain on the very day that his first article on possible diazo compounds appeared in print: “A Preliminary Notice on the Influence of Nitrous Acid on Aminonitro- and Aminodinitrophenol.” Griess’ first several attempts at diazotization exploded, but his commission at the Royal College was to investigate his new nitrogen intermediates, with the result that diazobenzoic acid was isolated and an entirely new class of compounds was discovered.(18, 25) Because many of these compounds were found to be stable and could be used...

Words: 4292 - Pages: 18

Premium Essay

Project 2 Aquatic Micro

...------------------------------------------------- Aquatic microbiology & sewage treatment Aquatic Microbiology refers to the study of microorganisms and their activities in natural waters, like lakes, ponds, streams, rivers, estuaries(brackish water), and oceans. Domestic and industrial wastewater enters lakes and streams and its effects on microbial life are important factors in aquatic microbiology. Also, how methods of treating wastewater mimics a natural filtering process. ------------------------------------------------- Aquatic microbiology & sewage treatment Aquatic Microbiology refers to the study of microorganisms and their activities in natural waters, like lakes, ponds, streams, rivers, estuaries(brackish water), and oceans. Domestic and industrial wastewater enters lakes and streams and its effects on microbial life are important factors in aquatic microbiology. Also, how methods of treating wastewater mimics a natural filtering process. BIO: 212 M7A3 Project 2 Dr. Ilse Silva-Krott BIO: 212 M7A3 Project 2 Dr. Ilse Silva-Krott OUTLINE: Aquatic Microbiology and Sewage Treatment I. Freshwater and Seawater habitats of microorganisms II. How wastewater pollution is a public health and ecological problem III. Causes and Effect of Eutrophication IV. How water is tested for bacteriological purity V. How pathogens are removed from drinking water VI. Compare primary, secondary, tertiary...

Words: 2421 - Pages: 10

Premium Essay

Health Promotion and Prevention

...NINTH EDITION Burton’s MICROBIOLOGY FOR THE HEALTH SCIENCES Paul G. Engelkirk, PhD, MT(ASCP), SM(AAM) Biomedical Educational Services (Biomed Ed) Belton, Texas Adjunct Faculty, Biology Department Temple College, Temple, TX Janet Duben-Engelkirk, EdD, MT(ASCP) Biomedical Educational Services (Biomed Ed) Belton, Texas Adjunct Faculty, Biotechnology Department Temple College, Temple, TX Acquisitions Editor: David B. Troy Product Manager: John Larkin Managing Editor: Laura S. Horowitz, Hearthside Publishing Services Marketing Manager: Allison Powell Designer: Steve Druding Compositor: Maryland Composition/Absolute Service Inc. Ninth Edition Copyright © 2011 Lippincott Williams & Wilkins, a Wolters Kluwer business © 2007 Lippincott Williams & Wilkins, © 2004 Lippincott Williams & Wilkins, © 2000 Lippincott Williams & Wilkins, © 1996 Lippincott-Raven, © 1992, 1988, 1983, 1979 JB Lippincott Co. 351 West Camden Street Baltimore, MD 21201 Printed in the People’s Republic of China All rights reserved. This book is protected by copyright. No part of this book may be reproduced or transmitted in any form or by any means, including as photocopies or scanned-in or other electronic copies, or utilized by any information storage and retrieval system without written permission from the copyright owner, except for brief quotations embodied in critical articles and reviews. Materials appearing in this book prepared by individuals as part of their official duties as U.S. government employees...

Words: 118758 - Pages: 476

Premium Essay

Microbiology

...Diagnosis of an Infected Patient: Microbiology Paper BIO 212 Professor Fazely January 18, 2015 Professor Fazely Bio 212 18 January 2015 Diagnosis of an Infected Patient After collecting a sputum sample from a patient that is suspected or may be infected with a bacterium from one of the following genera: Escherichia, Mycoplasma or Bacillus; Each bacteria listed should be isolated by utilizing each of the various staining techniques. The best staining techniques to use is the Gram stain or the Acid- fast stain due to the fact that they both will provide a lot of information in detail regarding the bacteria being studied. It is very important to be observant of how each bacterium obtained reacts to each stain, and how the results obtained will lead us in a developing diagnosis. The first technique being used is the Gram stain. Gram stain is probably one of the most common used staining procedures used in the field of microbiology. It is one of the differential stains that are used to characterize bacteria in one of the two groups: either gram positive or gram negative bacteria. Bacteria prepared for the Gram stain is a heated fixed smear that is covered with a crystal violet. Because the purple stain impart its color to all cells. After a short period of time, the purple dye is then washed off, and the smear is then covered with iodine, a mordant. When the iodine is then washed off, both the gram-positive and gram-negative bacteria appear dark violet in color or...

Words: 931 - Pages: 4

Premium Essay

Environmental and Food Microbiology Repor

...Introduction: The microbiology of food and the environment are two very important fields in the large scope of microbiological research. Because microorganisms exist almost everywhere, it is important to determine the influences that they place on the food we depend on for survival, and the environment in which we humans call home. In this lab, we conducted five experiments in these two fields, and in doing so gained a better understanding of the influences and importance of microbes in food and the environment. The first exercise was the enumeration of soil microbes. This experiment showcased the immense diversity of bacteria, actinomycetes, and fungi found in soil. This diversity ranges from microbes that are beneficial to the environment by decomposing dead organic matter into energy sources usable by other organisms, to the pathogenic bacterial and fungal spores that can infect humans and animals alike. The techniques used are serial dilutions, which allow for quantification and a close estimation of the amount of said organisms found in a soil sample. (1) The second exercise that we conducted was the microbiology of water experiment. This is a very important standardized experiment used to determine the density of coliforms found in a 100 mL sample of water. It also can be used more specifically to determine the density of Escherichia coli, which can cause food poisoning amongst other illnesses. The techniques used are the multiple tube fermentation method, which involves...

Words: 3244 - Pages: 13

Premium Essay

Microbiology

...Diagnosis of an Infected Patient: Microbiology Paper Bio 212 18 January 2015 Diagnosis of an Infected Patient After collecting a sputum sample from a patient that is suspected or may be infected with a bacterium from one of the following genera: Escherichia, Mycoplasma or Bacillus; Each bacteria listed should be isolated by utilizing each of the various staining techniques. The best staining techniques to use is the Gram stain or the Acid- fast stain due to the fact that they both will provide a lot of information in detail regarding the bacteria being studied. It is very important to be observant of how each bacterium obtained reacts to each stain, and how the results obtained will lead us in a developing diagnosis. The first technique being used is the Gram stain. Gram stain is probably one of the most common used staining procedures used in the field of microbiology. It is one of the differential stains that are used to characterize bacteria in one of the two groups: either gram positive or gram negative bacteria. Bacteria prepared for the Gram stain is a heated fixed smear that is covered with a crystal violet. Because the purple stain impart its color to all cells. After a short period of time, the purple dye is then washed off, and the smear is then covered with iodine, a mordant. When the iodine is then washed off, both the gram-positive and gram-negative bacteria appear dark violet in color or purple. The next, process is the slide is then washed with alcohol...

Words: 921 - Pages: 4

Premium Essay

Micro

...1. What were the historical scientific breakthroughs that led to our current understanding of viruses, viroids, and prions? Researchers discovered viruses by studying a plant disease. The discovery of viruses resulted from the search for the infectious agent causing tobacco mosaic disease. In 1935, Wendell Stanley isolated tobacco mosaic virus, making it possible to carry out chemical and structural studies on a purified virus. Viroids are infectious pieces of RNA that cause some place diases such a potato spindle tuber disease. In 1980s Prions were first discovered as infectious proteins. Prions are pathogens that are proteins, and they appear to cause a number of degenerative brain diseases, such as Scrapiein sheep, mad cow disease, and Creutzfeldt-Jakob disease in humans. 2. How do viruses, viroids, prions, and bacteria differ in terms of their biological identity and method of infection? Viruses contain both nucleic acids such as DNA or RNA and a protein coat. Viruses cause infection by inserting their genetic material in a host cell. The host cell then reproduces and reads the genetic material, making new viruses that eventually burst out of the cell and leave to infect more. The reason viruses cause disease is that the infected cells can’t carry out the normal functions necessary. Viroids on the other hand have no protein coat, but are only the nucleic acid RNA. Since they have no protein coat and are only the nucleic acid RNA they are often carried around inside...

Words: 1177 - Pages: 5

Premium Essay

Diagnoses of an Infected Patient

...Diagnoses of an Infected Patient By: Reathea Felder Microbiology Excelsior college | | When given a sputum sample it is important to take in consideration the type of bacteria that could be present, causing illness in the patient. There are hundreds of different types of bacteria, but I will only focus on how to detect three of them: Bacillus, Escherichia, and Mycoplasm. I will also discuss how to identify the genera causing the patients infection by describing the different staining procedure protocols. The term bacillus has two different meanings. The lowercase bacillus means bacterial shape while the italicized bacillus refers to a specific genus. Bacillus cells are usually single rods that may look like straws that often form long twisted cells (p. 78). They are typically rods that produce endospores. Bacillus is a gram-positive cell that may at times have gram negative cells. In a gram positive cell, there are many layers of a thick, rigid structure known as peptidoglycan. They also have teichoic acids which are made primarily of alcohol and phosphate. This acid plays a role in cellular growth and prevention of cell wall breakdown. One of the most well known bacteria in microbiology, Escherichia can be characterized as rod shaped having short hair like appendages. Escherichia is a gram negative...

Words: 848 - Pages: 4

Premium Essay

Architecture Research Outline

...INTRODUCTION: Major changes were made in architecture, while scientists were slowly beginning to create different cures for infectious diseases. Rival styles were created and architects were well known as artists. With discovering new medical advances, science was like a trip into the future. Sculpting and carving was well used for the beautification process, which revolutionized the eighteen-hundreds. The growth of microbiology and biochemistry had been linked to ways of finding new methods of killing deadly bacteria. ARCHITECTURE: A. Nineteenth Century: The Victorian Period lasted for approximately sixty-nine years. The love of this rare form of architecture quickly spread across England. Many revived styles of the past were used, but so were new methods and materials. This idea of basing entire buildings on ancient models revived Greek and Gothic styles. B. Changes in Patronage: There were changes in patronage for the middle class that included the spread of new wealth. To celebrate this and show the world what they could do, the middle class built abundantly; and thus antiquarianism was born. C. Beautification: Many buildings were made to appear less unorthodox than they really were by facades and embellishments. Some ideas that made the eighteenth century so unique were their classical front pieces, and Georgian pediments. This was not only to show beauty but also grace and their monumental significance. GOTHIC REVIVAL PHASES: A. Romantic: In this...

Words: 479 - Pages: 2