...Forensic Hair Analysis Forensic hair analysis is a method of studying hair found at the scene of a crime, and the scientific study of hair is referred to Trichology. In this paper I will discuss the different techniques that are used to study hair during an investigation and the many different aspects of the hair to consider based on what type of investigation is taking place. Hair analysis can be used to determine the presence of a suspect at the scene or to detect the presence of certain chemical substances in the victim or suspect. With the help of DNA techniques used in forensic hair analysis an absolute match can be determined by examining hair found at the scene of a crime. According to Douglas Deedrick of the FBI’s Trace Evidence Unit, Humans shed an average of 100 hairs daily and are often used in violent crime investigations such as: homicides, assaults, burglaries, and armed robberies. (Deedrick, 2000) The most useful hairs in a crime scene investigation are longer hairs found on the scalp but can be subject to changes due to hair colorings or a simple haircut. In order to prevent this outside exposure samples should be taken from potential suspects as soon as possible after the crime to avoid any possible contamination. According to Deedrick, examining a hair under a microscope can help to determine many things about the source of the hair and even how the hair was lost either through shedding or by force, or from which location on the body the hair originated...
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...Locard's Exchange Principle, and the limits of Forensic Science. Various case studies will also be analysed throughout the lecture. Case Studies Felicia Lee; Walter Dinivan; Madam Jetkor Miang Singh; Roberto Calvi; Buck Ruxton & the Jigsaw Murders; Acid Bath Haigh; 2005 London Bombings; "Brides in the Bath"; Gareth Williams; The Woodchipper Murder WEEK 2- CHEMICAL ANALYSIS IN FORENSIC SCIENCE Quote "Actus non facit reum nisi mens sit rea" The act is not culpable unless the mind is also guilty. Learning Objective(s) At the end of this topic, you should be able to: 2A. Atomic Structure & Spectroscopy 1. Explain the structure of the atom and Bohr's model; 2. Differentiate between emission and absorption spectroscopy; 3. Explain the chemistry behind EDX and SEM-EDX; 4. Explain the chemistry in NAA; 2B. Molecular Spectroscopy & Chromatography 1. Apply the principles of chromatography; 2. Describe the methods used in the analysis of compounds; 3. Explain the chemistry behind the methods for the analysis of compounds; 4. Identify which method is more effective for the analysis of compounds. Synopsis An introduction to spectroscopy and the structure of atoms. The comparisons...
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...FORENSICS Forensics, by and large, is the application of science to the legal process. It is an emerging research domain in India. There are many different types of forensic sciences baring their vital presence possibly in every field of human endeavor. Of these, let us now discuss about the computational, cyber and the DNA forensics. COMPUTATIONAL FORENSICS: The development of computational methods or mathematical and software techniques to solve forensic issues is called computational forensics. These methods analyze the evidence beyond human cognitive ability. They scrutinize a large volume of data, which is at any case impossible for a human mind to figure out. In spite of this, we can’t say that these techniques alone would serve our purpose because computational forensics is a field which needs huge collaboration between recognition and reasoning abilities of humans combined with comprehension and analytic abilities of the tool or a machine, which is most of the times, a computer. Computational forensics aids us to model the uncertain. At the crime scenes, we usually get incomplete or broken evidences. These evidences are later on modeled by the computational forensic tool which gives us first clues from its largest biometric database (fingerprints, criminal histories, mug-shots, scar and tattoo, physical characteristics like height, weight, hair and eye color and aliases), which is a collection of significant information regarding the criminals, their criminal history...
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...for smallholder farmers in the Philippines. Social and economic factors might prevent households from diagnosing, treating, and eliminating from circulation those animals or animal products, such as milk, that are afflicted with mastitis or which came from individuals afflicted with mastitis; this is especially true in the case of the asymptomatic subclinical mastitis, which tends to become chronic and difficult to eradicate by conventional antimicrobial therapies (Brouillette & Malouin, 2005; Ng et al., 2010). With the advent of technology comes new techniques in identifying and treating diseases such as mastitis through more rapid, accurate, and efficient means, such as fluorescent dye staining or the utilization of electrical fields (Dohoo & Meek, 1982). By far, genetic approaches to the treatment of this disease have been of great interest, primarily because of the lack of information regarding their application in...
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...integer, the function chosen would be to seek a match against a list of employee numbers; if the variable were a string, it would seek a match against a list of names. In either case, both functions would be known in the program by the same name. This type of polymorphism is sometimes known as overloading. 3) Polymorphism can mean, as in the ML language, a data type of "any," such that when specified for a list, a list containing any data types can be processed by a function. (For example, if a function simply determines the length of a list, it doesn't matter what data types are in the list.) 4) In PHP, polymorphism means that if B is a descendant of A and a function can accept A as a parameter, it can also accept B. We describe a new basis for the construction of a genetic linkage map of the human genome. The basic principle of the mapping scheme is to develop, by recombinant...
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...DNA Barcode sequence of marine ornamental Squat shrimp Thor amboinensis using mtCO1 from Gulf of Mannar coastal waters V.Priyalakshmi1, S.Dhanasekaran1, M.A.Badhul Haq2*, M.Nirosh Banu2, S.Vaitheeswari2 and P.Vengadesan2 1P.G and Research Department of Zoology, Yadava College, Madurai Kamaraj University, 2Marine Virology Laboratory, Faculty of Marine Sciences, CAS in Marine Biology, Annamalai University, Parangipettai - 608 502, Email*:drhaqmarinevirology@gmail.com Abstract Marine shrimp are among the most popular invertebrates in the marine ornamental aquarium trade. In this study exclusively focused in addressing the phylogenetic location of genus Thor amboinensis. A molecular phylogeny of the marine ornamental shrimp species based on...
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...adjusted to 30 mM with NaOH, and pH was adjusted to 7.0 with N-methylglucamine (NMG) or HCl. PIP2 liposomes (20–200 nm) were prepared by sonicating 1 mM PIP2 (Boehringer Mannheim) in distilled water. Reconstituted monoclonal PIP2 antibody (Perspective Biosystems, Framingham, MA) was diluted 40-fold into experimental solution. Current–voltage relations of all currents reversed at EK and showed characteristic rectification, mostly owing to the presence of Na+ in FVPP and possibly also residual polyamines. Current records presented (measured at 30 C, −30 mV holding potential) are digitized strip-chart recordings. Purified bovine brain Gbg29 was diluted just before application such that the final detergent (CHAPS) concentration was 5 M. Detergent-containing solution was washed away thoroughly before application of PIP2, because application of phospholipid vesicles in the presence of detergent usually reversed the effects of Gbg; presumably, Gbg can be extracted from membranes by detergent plus phospholipids. Molecular biology. R188Q mutation was constructed by insertion of the mutant oligonucleotides between the Bsm1 and BglII sites of pSPORT– ROMK1 (ref. 11). A polymerase chain reaction (PCR) fragment (amino acids 180–391) from pSPORT–ROMK1 R188Q mutant was subcloned into pGEX2T vector (Pharmacia) for expression of R188Q mutant protein of GST–RKC. The construction, expression and purification of GST–IKC (amino acids 182– 428 of IRK1), GST–GKC (180–462 of GIRK1), GST–IKN (1–86 of IRK1) have...
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...1. Write an essay on protein structure and synthesis Protein synthesis is a cellular process leading to the production of proteins. This term is also synonymous to protein translation. It begins with a sequential process of transcription of DNA into mRNA, which is then used as input for translation after exon-intron splicing. The addition of successive tRNA molecules based on the code of mRNA matched up by base-pairing through their anti-codons in the ribosomes creates the nascent protein. After the protein chain has been synthesized, post-translation modification occurs, e.g. phosphorylation, motifs added to the protein. This may happen at various levels: secondary (alpha-helix, beta-sheets, turn, random coiling), tertiary and quarternary. Protein synthesis is also the process wherein peptide bonds between two amino acids whose formation is controlled. The synthesis begun when the mRNA combines in a little subunit of ribosomes close to an AUG sequence in mRNA. Start codon which is the AUG codon is being such because it does the coding of the first amino acid to be made of protein. “The AUG codon base-pairs with the anticodon of tRNA carrying methionine. A large ribosomal subunit binds to the complex, and the reactions of protein synthesis itself can begin. The aminoacyl-tRNA to be called for next is determined by the next codon (the next three bases) on the mRNA. Each amino acid is coded for by one or more (up to six) codons” (Center for Bioenergy and Photosynthesis...
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...What is Cloning? Clones are organisms that are exact genetic copies. Every single bit of their DNA is identical. Clones can happen naturally—identical twins are just one of many examples. Or they can be made in the lab. Below, find out how natural identical twins are similar to and different from clones made through modern cloning technologies. How Is Cloning Done? Many people first heard of cloning when Dolly the Sheep showed up on the scene in 1997. Artificial cloning technologies have been around for much longer than Dolly, though. There are two ways to make an exact genetic copy of an organism in a lab: artificial embryo twinning and somatic cell nuclear transfer. 1. Artificial Embryo Twinning Artificial embryo twinning is a relatively low-tech way to make clones. As the name suggests, this technique mimics the natural process that creates identical twins. In nature, twins form very early in development when the embryo splits in two. Twinning happens in the first days after egg and sperm join, while the embryo is made of just a small number of unspecialized cells. Each half of the embryo continues dividing on its own, ultimately developing into separate, complete individuals. Since they developed from the same fertilized egg, the resulting individuals are genetically identical. Artificial embryo twinning uses the same approach, but it is carried out in a Petri dish instead of inside the mother. A very early embryo is separated into individual cells, which are allowed...
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...... 1 1.1 Phylogeography....................................................................................................... 1 1.2 Palaeodrainage........................................................................................................ 4 1.3 Background on Study Species .................................................................................. 6 1.4 Aims and Hypothesis ............................................................................................... 8 2. Materials and Methods ............................................................................................... 10 2.1 Locations ............................................................................................................... 10 2.2 Expectations of Hypothesis.................................................................................... 12 2.3 Sample Collection and Storage .............................................................................. 13 2.4 DNA Extraction ...................................................................................................... 13 2.5 Pilot study...
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...REGULATION OF PHYSIOLOGICAL SYSTEMS BY NUTRIENTS Free Radicals, Antioxidants, and Nutrition Yun-Zhong Fang, Sheng Yang, and Guoyao Wu, PhD From the Department of Biochemistry and Molecular Biology, Beijing Institute of Radiation Medicine, Beijing, China; the Division of Animal Nutrition, Department of Animal Science, China Agricultural University, Beijing, China; and the Department of Animal Science and Faculty of Nutrition, Texas A&M University, College Station, Texas, USA Radiation hazards in outer space present an enormous challenge for the biological safety of astronauts. A deleterious effect of radiation is the production of reactive oxygen species, which result in damage to biomolecules (e.g., lipid, protein, amino acids, and DNA). Understanding free radical biology is necessary for designing an optimal nutritional countermeasure against space radiation–induced cytotoxicity. Free radicals (e.g., superoxide, nitric oxide, and hydroxyl radicals) and other reactive species (e.g., hydrogen peroxide, peroxynitrite, and hypochlorous acid) are produced in the body, primarily as a result of aerobic metabolism. Antioxidants (e.g., glutathione, arginine, citrulline, taurine, creatine, selenium, zinc, vitamin E, vitamin C, vitamin A, and tea polyphenols) and antioxidant enzymes (e.g., superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidases) exert synergistic actions in scavenging free radicals. There has been growing evidence over the past three decades...
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...Organism * The application of genetic analysis and DNA technology to the study of development has brought about a revolution in our understanding of how a complex multicellular organism develops from a single cell. * In 1995, Swiss researchers identified a gene that functions as a master switch to trigger the development of the eye in Drosophila. * A similar gene triggers eye development in mammals. * Developmental biologists are discovering remarkable similarities in the mechanisms that shape diverse organisms. * While geneticists were advancing from Mendel’s laws to an understanding of the molecular basis of inheritance, developmental biologists were focusing on embryology. * Embryology is the study of the stages of development leading from fertilized egg to fully formed organism. * In recent years, the concepts and tools of molecular genetics have reached a point where a real synthesis of genetics and developmental biology has been possible. * When the primary research goal is to understand broad biological principles, the organism chosen for study is called a model organism. * Researchers select model organisms that are representative of a larger group, suitable for the questions under investigation, and easy to grow in the lab. * For study of the connections between genes and development, suitable model organisms have short generation times and small genomes that are suitable for genetic analysis. * Model...
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...Introduction Human embryonic stem cells (hES cells) are currently discussed not only by the biologists by whom they were discovered but also by the medical profession, media, ethicists, governments and politicians. There are several reasons for this. On the one hand, these ‘super cells’ have a major clinical potential in tissue repair, with their proponents believing that they represent the future relief or cure of a wide range of common disabilities; replacement of defective cells in a patient by transplantation of hES cell‐derived equivalents would restore normal function. On the other hand, the use of hES cells is highly controversial because they are derived from human pre‐implantation embryos. To date, most embryos used for the establishment of hES cell lines have been spare embryos from IVF, but the creation of embryos specifically for deriving hES cells is also under discussion. The most controversial variant of this is the transfer of a somatic cell‐nucleus from a patient to an enucleated oocyte (unfertilized egg) in order to produce hES cells genetically identical to that patient for ‘autologous’ transplantation (so‐called ‘therapeutic’ cloning); this may prevent tissue rejection. The question ‘Can these cells be isolated and used and, if so, under what conditions and restrictions’ is presently high on the political and ethical agenda, with policies and legislation being formulated in many countries to regulate their derivation. The UK has been the first to pass...
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...Chapter 10 Chapter 11 Chapter 12 Chapter 13 Chapter 14 Chapter 15 Chapter 16 Chapter 17 Chapter 18 Chapter 19 Chapter 20 Chapter 21 Chapter 22 Chapter 23 Introduction to Biochemistry Water Amino Acids and the Primary Structures of Proteins Proteins: Three-Dimensional Structure and Function Properties of Enzymes Mechanisms of Enzymes Coenzymes and Vitamins Carbohydrates Lipids and Membranes Introduction to Metabolism Glycolysis Gluconeogenesis, The Pentose Phosphate Pathway, and Glycogen Metabolism The Citric Acid Cycle Electron Transport and Oxidative Phosphorylation Photosynthesis Lipid Metabolism Amino Acid Metabolism Nucleotide Metabolism Nucleic Acids DNA Replication, Repair, and Recombination Transcription and RNA Processing Protein Synthesis Recombinant DNA Technology 1 10 27 46 65 85 104 119 137 153 169 185 199 213 227 241 256 269 284 300 315 330 348 Chapter 1 Introduction to Biochemistry 1) Which elements account for more than 97% of the weight of most organisms? A) C, H, N, Mg, O, S B) C, H, N, O, P, S C) C, H, N D) Fe, C, H, O, P E) Ca2+ , K+ , Na+ , Mg 2+ , ClAnswer: B Page Ref: Section 2 2) Proteins in biological membranes may be A) porous. B) attached to the membrane surface. C) span the membrane. D) All of the above E) B and C only Answer: D Page Ref: Section 3 3) Which statement about cellulose is false? A) It is the most abundant polysaccharide in nature. B) Its monomers are joined...
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...Carbondale OpenSIUC Publications Department of Anthropology 6-1-2004 An Integrated Analysis of Pre-Hispanic Mortuary Practices: A Middle Sicán Case Study Izumi Shimada Southern Illinois University Carbondale Ken-ichi Shinoda National Science Museum, Tokyo Julie Farnum Montclair State University Robert Corruccini Southern Illinois University Carbondale Hirokatsu Watanabe Terra Information Engineering Company Follow this and additional works at: http://opensiuc.lib.siu.edu/anthro_pubs © 2004 by The Wenner‐Gren Foundation for Anthropological Research Published in Current Anthropology, Vol. 45, No. 3 ( June 2004) at 10.1086/382249 Recommended Citation Shimada, Izumi; Shinoda, Ken-ichi; Farnum, Julie; Corruccini, Robert; and Watanabe, Hirokatsu, "An Integrated Analysis of PreHispanic Mortuary Practices: A Middle Sicán Case Study" (2004). Publications. Paper 8. http://opensiuc.lib.siu.edu/anthro_pubs/8 This Article is brought to you for free and open access by the Department of Anthropology at OpenSIUC. It has been accepted for inclusion in Publications by an authorized administrator of OpenSIUC. For more information, please contact jnabe@lib.siu.edu. C u r r e n t A n t h r o p o l o g y Volume 45, Number 3, June 2004 2004 by The Wenner-Gren Foundation for Anthropological Research. All rights reserved 0011-3204/2004/4503-0004$3.00 An Integrated Analysis of PreHispanic Mortuary Practices A Middle Sican Case Study1 ´ by Izumi Shimada, Ken-ichi...
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