...2014-2018 Global Polymerase Chain Reaction (PCR) Market EMISPDF us-thermo-url from 208.89.140.11 on 2014-11-24 17:09:41 GMT. DownloadPDF. technavio insights Downloaded by us-thermo-url from 208.89.140.11 at 2014-11-24 17:09:41 GMT. EMIS. Unauthorized Distribution Prohibited. 2014-2018 Global Polymerase Chain Reaction (PCR) Market Table of Contents 01. Executive Summary.......................................... 1 02. List of Abbreviations ......................................... 2 03. Scope of the Report.......................................... 3 03.1 Market Overview .......................................................... 3 03.2 Product Offerings .......................................................... 3 04. Market Research Methodology ..................... 6 04.1 Market Research Process .......................................... 6 04.2 Research Methodology .............................................. 6 05. Introduction ....................................................... 8 06. Market Landscape ........................................... 9 06.1 Market Overview .......................................................... 9 06.2 Market Size and Forecast........................................... 9 06.3 PCR Market in US ........................................................ 10 06.3.1 Mark et Size and Forecast .................................................. 10 06.4 PCR Market in Europe......................................
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...DNA Fingerprinting Using Agarose Gel S. Aaron Sowards Bio 122 Lab 04 Brianna Adanitsch Jakob Lester Minhenga Ngijoi 2/21/18 Dr. Chad R. Sethman Abstract DNA fingerprinting is the process of analyzing an individual’s DNA base-pair patterns. The DNA fingerprinting lab involved identifying the suspect using Agarose Gel and Polymerase Chain Reaction. It was found that suspect two s DNA matched the crime scene DNA. This is known because suspect twos DNA traveled the same distance as the crime scene DNA. DNA Fingerprinting Using Agarose Gel Introduction In 1984 Dr. Alex Jeffreys came up with deoxyribonucleic acid (DNA) fingerprinting, which is also known as DNA profiling or DNA typing. DNA fingerprinting is the analyzing...
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...Someone’s Name Bio 488C Lab A Re: Writing Assignment 2: Enterics Report 2/22/2016 Enterics Report The group received the Unknown sample of Patient #2 as well as a description of the illness. The method to identify this unknown organism was an Enteropluri tube. By using the inoculation needle in the Enteropluri tube to sample the organism and draw the sample through the set of agar compartments, multiple culture media are thereby inoculated. Given that this test is used on Enterobacteriaceae all organisms are assumed to be Gram negative and oxidase negative. However, each culture media provides a biochemical test that is used to differentiate and identify the microorganism such as glucose fermentation with or without gas production, lactose fermentation, urea hydrolysis, etc. Each feature is used to differentiate and identify the microorganism in question. While the Enteropluri tube is a useful device, I do not believe it is the most effective method of identification in a modern clinical setting. Possible drawbacks of this device is failing to get a uniform inoculation of each media – as the inoculation needle is drawn through each successive media less and less of the sample is present, meaning the first compartments inoculated may receive more cells which will result in quicker growth, compaired to the final compartments which may receive far fewer cells and result in slower growth and possible false negatives. There is also a concern that the media in each compartment...
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...Epidemic Paper Ebola In the late 2014 and early 2015 there was this great outbreak of deadly Ebola in many countries in West Africa. The Ebola virus disease was formerly referred as Ebola hemorrhagic fever. The outbreak has happened in the past, but the recent one was a massive. It proclaimed much life that the west was a no-go zone due to fear of transmission. Due to the collaboration of many world health organizations and non-government organizations the epidemic was controlled and maintained. An epidemic disease is a disease that on outbreak it proclaims many lives in a short period of time before been controlled (Chowell et al. 2015). Ebola, as stated, is caused by a virus pathogen, it a virus is from Filoviridae family which include three main genera: Cuevavirus, Marburgvirus, and Ebolavirus. These are the main viruses responsible for the massive outbreak of Ebola last year in West Africa. For an extensive period of the spell, it is believed that the virus originated from infected animals and transmitted to human through food. Fruits bat of the Pteropodidae family is seen as the carrier of the Ebola virus. The virus was introduced into the human population through close contact with body fluids like blood, saliva, and sweat. From one human to the other the virus is transmitted via direct contact with bodily fluids or material, which had a contact with, infected people (Chowell et al. 2015). The virus is such a deadly to the extent that it resides even in dead bodies...
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...H e p a t i t i s M o n t h l y 2 0 0 7 ; 7 ( 3 ) : 1 5 3 -1 6 2 1 Hepatitis C among Hemodialysis Patients: A Review on Epidemiologic, Diagnostic, and Therapeutic Features Seyed-Moayed Alavian 1, Seyed Mohammad-Mehdi Hosseini-Moghaddam 2*, Mohammad Rahnavardi 2 M M M 1 Baqiyatallah Research Center for Gastroenterology and Liver Diseases, Baqiyatallah University of Medical Sciences & Tehran Hepatitis Center, Tehran, Iran 2 Urology and Nephrology Research Center (UNRC), Shaheed Beheshti University of Medical Sciences, Tehran, Iran Hepatitis C virus (HCV) is a major public health problem and is the most common liver disease among hemodialysis (HD) patients. The seroprevalence of HCV infection among HD ranged from 1.9% to 80% in reports published since 1999. The main risk factor for HCV acquisition in HD patients seems the length of time on HD. Phylogenetic analysis of HCV viral isolates has suggested nosocomial patient-to-patient transmission of HCV infection among HD patients. Lack of strict adherence to universal precautions by staff and sharing of articles such as multidose drugs might be the main mode of nosocomial HCV spread among HD patients. Currently, there are several dilemmas on the management of these patients: should HCV-RNA testing be included in the routine screening of HD population for HCV infection?; does periodic serum alanine aminotransferase testing have a role in screening HD patients for HCV infection?; can dialysis really 'save' the liver of HCV-infected...
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...Coccioidiodal Mycosis Coccioidiodal Mycosis According to Fauci et al., fungal infections are classified into categories based on anatomic location and epidemiology. The most frequent anatomic categories are mucocutaneous and deep organ infections. The most common epidemiologic categories are endemic and opportunistic. The endemic mycoses, such as coccidioidomycosis, are infections caused by fungal organisms that are not found in normal human flora and are instead acquired from environmental sources. In contrast, organisms found in normal human microbial flora cause opportunistic infections. Endemic fungal infections are acquired almost exclusively by inhalation of molds in the environment. Soil, dust, and dirt are the natural reservoirs for most of these infections, demonstrated by an increase in cases following dust storms, seismic events, archeological digging, or recreational activities (Fauci et al., 2008). The incidence of endemic fungal infections has risen substantially over the past several decades, especially in geographic locations in which there has been substantial population growth (Fauci et al., 2008). Healthcare providers may be required to recognize and treat an increasing number of severe coccidioidal infections as growth and urbanization to these areas increases. Additionally, a recent study of community-acquired pneumonia (CAP) in Arizona suggested coccidioidomycosis might be a...
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...Eyeless mutation gene located within the second intron of Drosophila melanogaster Justin Lazarus Genetic 300 Abstract The following experiment was conduct over a several week time span to determine and identify the mutation that is causing the eyeless mutation within the Drosophila melanogaster fruit flies. The experiment included genome sequencing and comparison between the Drosophila melanogaster wild type and the Drosophila melanogaster eyeless type. After combining the two different phenotypes. We determined that we were unable to visualize the mutation at a chromosomal level, as both wild-type and eyeless flies looked similar. The experiment involved electrophoresis and Polymerase Chain Reaction (PCR) through which we were able to isolate and amplify the needed DNA eyeless DNA. The difference between the wild-type Drosophila melanogaster and the eyeless Drosophila melanogaster is approximately only 500-nucleotide base pairs. As we see the eyeless phenotype is approximately 3000 base pairs in length while the wild-type phenotype is approximately 2500 nucleotides base pairs in length, a difference of about 500 base pairs. After completing nucleotide sequencing and comparing our data on the blast website, we determined that the eyeless mutation has being interest exons two and three, but more specifically the mutation itself was located within the second intron at base pairs 8264 to 9212. Introduction In the early 20th century scientists had already been acquainted with...
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...Major Crime Unit : NH State Police Summer Internship | Heating up: Cold Cases | Meredythe Leonard | | Heating Up: Cold Cases Countless developments in police science have occurred in the past decade or so. Many of these improvements can be seen as small, but imagine the challenges faced by crime scene investigators 50 or more years ago. The law enforcement agencies of the past did not have routine access to the amount information that officials today can gather and analyze from a crime scene. Current day crime scene investigation can range from the downright tedious to the technologically astounding, but they have all greatly impacted how evidence is collected, documented, and analyzed. There are around one hundred unsolved homicides in New Hampshire alone, dating back to the early 1960’s. (Department of Justice, 2015). Evidence from those crimes could quite possibly be the golden ticket in solving the wrongdoing, but with outdated techniques and capabilities, the answer will remain a mystery. If the state makes older evidence testing a priority, cold cases could finally find the justice deserved with the help of current day technology. Throughout the rest of this discussion, the call for new evidence testing in cold cases is examined while highlighting the need and importance for skilled investigators. Determination and patience, in the advancing field of forensic testing, have unlocked numerous cases not only in New Hampshire, but also across the country...
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...LAB REPORT BLOODSTAIN ANALYSIS (http://www.crimescene-forensics.com/images/Blood_Spatter_001B.gif) COURSE: CHM 1107: Introduction to Forensic Science - Professor Ira S. Krull DATE: 10.27.11 INTRODUCTION Blood is a biological fluid found in animals that delivers necessary nutrients and oxygen throughout the body and carries away metabolic waste products. The average adult has a blood volume of roughly 5 liters, comprising of plasma, blood cells and platelets, and it is usually red in color, though not always. The most abundant cells in vertebrate blood are red blood cells, constituting about 45% of whole blood. These cells contain hemoglobin, an iron-containing protein, which distributes oxygen throughout the human body. Blood is circulated around the body through blood vessels by the pumping action of the heart. In humans, blood is pumped from the strong left ventricle of the heart through arteries to peripheral tissues and returns to the right atrium of the heart through the superior vena cava (see Fig 1). It then enters the right ventricle and is pumped through the pulmonary artery to the lungs and returns to the left atrium through the pulmonary veins. Blood then enters the left ventricle to be circulated again. (Fig 1: http://upload.wikimedia.org/wikipedia/commons/thumb/e/e5/Diagram_of_the_human_heart_%28cropped%29.svg/220px-Diagram_of_the_human_heart_%28cropped%29.svg.png) Blood is a type of evidence found most often at violent crime scenes and...
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...DNA profiling (also called DNA testing, DNA typing, or genetic fingerprinting) is a technique employed by forensic scientists to assist in the identification of individuals by their respective DNA profiles. DNA profiles are encrypted sets of numbers that reflect a person's DNA makeup, which can also be used as the person's identifier. DNA profiling should not be confused with full genome sequencing.[1] It is used in, for example, parental testing and criminal investigation. Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different to distinguish one individual from another, unless they are monozygotic twins.[2] DNA profiling uses repetitive ("repeat") sequences that are highly variable,[2] called variable number tandem repeats (VNTR), particularly short tandem repeats (STR)s. VNTRs loci are very similar between closely related humans, but so variable that unrelated individuals are extremely unlikely to have the same VNTRs. The DNA profiling technique was first reported in 1984[3] by Sir Alec Jeffreys at the University of Leicester in England,[4] and is now the basis of several national DNA databases. Dr. Jeffreys's genetic fingerprinting was made commercially available in 1987, when a chemical company, Imperial Chemical Industries (ICI), started a blood-testing centre in England.[5] Contents [hide] 1 DNA profiling process 1.1 RFLP analysis 1.2 PCR analysis 1.3 STR analysis 1.4 AmpFLP ...
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...according to New York Times. Enteroviruses are spread through bodily fluids from coughing or sneezing and food or water that is contaminated by very minimal, trace amounts of fecal matter. Herpes Simplex virus. Worldwide, herpes simplex encephalitis is the most common cause of non-epidemic encephalitis (Banatvala, pg 80). The herpes virus group includes a number of common infections, including herpes simples, varicella-zoster (cause of chickenpox and shingles) and others. About 2,100 people are hospitalized each year from herpes-associated encephalitis. (Photo shows “axial diffusion-weighted image reveals restricted diffusion in left medial temporal lobe consistent with herpes encephalitis. This patient also had positive result on polymerase chain reaction assay for herpes simplex virus, which is both sensitive and specific. In addition, patient had periodic lateralized epileptiform discharges on electroencephalography, which supports diagnosis of herpes encephalitis”. Source: http://emedicine.medscape.com) These viruses share certain features, including the capacity to cause an infection and then to go into hiding (New York Times). They can lie dormant for periods of time as short as months or as long as a lifetime. In most cases encephalitis from these viruses occur in people with impaired immune systems such as those with Human Immunodefiency Virus (HIV) (New York Times). Herpes simplex virus is the most common cause of encephalitis in developed countries. It’s responsible for...
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...With the invention of a technique to amplify DNA, polymerase chain reaction (PCR), experts can now make DNA fingerprints from even one trillionth of a gram of DNA. The beginnings of DNA profiling are credited to Sir Alec Jeffreys, of Leicester, England in 1977. He and his coworkers were studying ways to resolve immigration and paternity suits by detailing the genetic links between individuals. He soon realized that his discovery could establish the identity of a person and called his technique genetic fingerprinting. Dr. Jeffreys demonstrated that a genetic fingerprint is specific to each individual and the pattern does not belong to any other person on earth except for identical twins. Jeffreys’ techniques were put to the test when law enforcement contacted him to assist in 2 local murder cases. He was...
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...Studies VII Practical 9 Cell Biology Studies IX Practical 10 Cell Biology Studies X - Experiment Description Page Writing of Lab Reports Identification of Biomolecules 5 13 Identification of Unknown Carbohydrate Solutions and Investigation of Action of Saliva and HCl in Carbohydrate Solution at Two Different Temperatures Investigation of the Effects of Catalase Concentration on Hydrogen Peroxide Decomposition 20 Synthesis of Starch Using an Enzyme Extracted from Potato Tuber Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Microscopy 27 Practical 6 Cell studies II Practical 7 Cell studies III Extraction of Cell Organelles by Cell Fractionation Determination of Solute Potential of Potato Cell Sap 47 Practical 8 Cell studies IV Effects of Different Treatments on Stained Potato Cells 64 Practical 9 Energetics I Respiration of Germinating Beans 67 Microscopic Examination of Cells at Various Stages of Plant Mitosis and Meiosis DNA, Mitosis and Meiosis Modelling 71 Respiration of Yeast 93 Practical 3 Enzyme studies I (Experiment 1) Optional: Practical 3 Enzyme studies I (Experiment 2) Practical 4 Enzyme studies II Practical 5 Cell studies I - - Practical 10 Energetics II Lab manual version 6_201505 FHSB1214 Biology I & FHSC1214 Fundamentals of Cell...
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...Studies VII Practical 9 Cell Biology Studies IX Practical 10 Cell Biology Studies X - Experiment Description Page Writing of Lab Reports Identification of Biomolecules 5 13 Identification of Unknown Carbohydrate Solutions and Investigation of Action of Saliva and HCl in Carbohydrate Solution at Two Different Temperatures Investigation of the Effects of Catalase Concentration on Hydrogen Peroxide Decomposition 20 Synthesis of Starch Using an Enzyme Extracted from Potato Tuber Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Microscopy 27 Practical 6 Cell studies II Practical 7 Cell studies III Extraction of Cell Organelles by Cell Fractionation Determination of Solute Potential of Potato Cell Sap 47 Practical 8 Cell studies IV Effects of Different Treatments on Stained Potato Cells 64 Practical 9 Energetics I Respiration of Germinating Beans 67 Microscopic Examination of Cells at Various Stages of Plant Mitosis and Meiosis DNA, Mitosis and Meiosis Modelling 71 Respiration of Yeast 93 Practical 3 Enzyme studies I (Experiment 1) Optional: Practical 3 Enzyme studies I (Experiment 2) Practical 4 Enzyme studies II Practical 5 Cell studies I - - Practical 10 Energetics II Lab manual version 6_201505 FHSB1214 Biology I & FHSC1214 Fundamentals of Cell...
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...experiments in this paper done through instruction in graduate course: Biotechnology laboratory in the natural sciences and mathematics department at the university of texas at dallas, RICHARDSON, TX 75080 march 2016 [Company name] | [Company address] ------------------------------------------------- γ-globin analysis by expression profiling through RT-qPCR, quantification through ELISA, and oxidative stress management analysis by FACS from KU812F cells under treatment by δ aminolevulinic acid, succinylacetone, and N-methyl mesoporphyrin Shaan Sarode, Jose Cordero, and Dr. Li Liu experiments in this paper done through instruction in graduate course: Biotechnology laboratory in the natural sciences and mathematics department at the university of texas at dallas, RICHARDSON, TX 75080 march 2016 [Company name] | [Company address] ------------------------------------------------- γ-globin analysis by expression profiling through RT-qPCR, quantification through ELISA, and oxidative stress management analysis by FACS from KU812F cells under treatment by δ aminolevulinic acid, succinylacetone, and N-methyl mesoporphyrin Shaan Sarode, Jose Cordero, and Dr. Li Liu ABSTRACT Hemoglobinopathies refer to a group of blood related disorders that encompass important disease such as thalassemia and sickle cell disease. Because many of these disease are hereditary more aggressive genetic therapies are showing promise as possible avenues of treatment. One such method is to re-express...
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