PRACTICAL REPORT NAME | Dylan Yong Chun Yen | PARTNER’S NAME | Ng Cheau Wen | PRATICAL GROUP | PG 15 | DATE OF PRATICAL SESSION | 18th June 2014 | PROGRAMME | Foundation in Science ( P stream ) | UNIT CODE | FHSB 1214 | UNIT DESCRIPTION | Biology I | YEAR &TRIMESTER OF STUDY | 2014 Trimester 1 | TITLE OF LAB REPORT | Practical 3: Investigation of Action of Saliva and 3M Hydrochloric Acid in Two Carbohydrate Solutions | LECTURER’S NAME | Ms. Bong Siew
Words: 992 - Pages: 4
At any given moment, all of the work being done inside any cell is being done by enzymes. If you understand enzymes, you understand cells. A bacterium like E. coli has about 1,000 different types of enzymes floating around in the cytoplasm at any given time. Enzymes have extremely interesting properties that make them little chemical-reaction machines. The purpose of an enzyme in a cell is to allow the cell to carry out chemical reactions very quickly. These reactions allow the cell to build things
Words: 598 - Pages: 3
Biochemistry Empirical procedure for purifying Enzyme X: 1. To begin the purification process, mix blue green algae with an appropriate quantity of a buffer and triturate through use of a mechanical crushing process. Centrifuge at 4 degrees C for 10 min at 5,000 rpm. Next, determine whether the supernatant and the precipitate has the highest specific activity of the desired enzyme. The specific activity is the ratio of biochemical activity to the weight or volume of total protein
Words: 313 - Pages: 2
Experiment 5 Enzyme Kinetics: The Effect of Yeast Alcohol Dehydrogenase and Coenzyme on the Rate of Oxidation of Ethanol. Results Raw data: Effect of Enzyme Concentration | Reagent | 1 | 2 | 3 | 4 | Buffer B | 2.5 ml | 2.4 ml | 2.3 ml | 2.2 ml | NAD+ | 200 μl | 200 μl | 200 μl | 200 μl | Dilute ADH | 200 μl | 300 μl | 400 μl | 500 μl | 6 M Ethanol | 100 μl | 100 μl | 100 μl | 100 μl | Time 0 sec. | 0.022 | 0.026 | 0.069 | 0.073 | Time 5 sec. | 0.028 | 0.038 | 0.110 | 0.114
Words: 1202 - Pages: 5
filtrates of a white rot fungus, Lachnocladium sp. This enzyme was purified by anion exchange and gel filtration chromatography. Laccase activity was determined using ABTS (2, 2’-azinobis-(3-ethylbenzthiazoline)-6-sulphonic acid) substrate. The culture filtrate had maximum laccase activity of 1.62 U/ml after 14 days of incubation. The purified laccase had an optimum temperature of 50 oC and its optimum pH was 6 for ABTS. The activity of this enzyme was enhanced by Fe2+, Cu2+, Zn2+and Ca2+, and was inhibited
Words: 3358 - Pages: 14
A Hydrophilic and Hydrophobic Organic Solvent Mixture Enhance Enzyme Stability in Organic Media Pepito A. Comeque Clinical Biochemistry / CLS 301 16 JAN 2014 Dr. Jared Thomas Rutledge A Hydrophilic and Hydrophobic Organic Solvent Mixture Enhance Enzyme Stability in Organic Media The enzymatic catalysis in organic solvents present many potential reaction that are impossible in aqueous solution, including chiral synthesis and resolution, the alteration of fats and oils and the creation
Words: 783 - Pages: 4
The effect of pH on salivary amylase Introduction: Saliva in the mouth contains the protein salivary amylase which acts on starches to break them down into mono- and disaccharides.(2) Saliva has been found to have an average pH level of about 6.78 +/- when tested in various locations of the mouth from multiple healthy individuals.(1) Saliva acts as a buffer against any possible changes in the pH from acids produced from bacteria, maintaining the oral cavity at an almost
Words: 999 - Pages: 4
The Discussion should be written after the Results section so that you have a good idea of what the experiment has demonstrated. The discussion section should definitely have a statement of your expected findings (Pechenik, 86). This should include your hypothesis and a brief statement about why these types of results are expected. There should also be a comparison of how your actual results related to your expected findings (Pechenik, 86). Here, you should state whether or not your results supported
Words: 1314 - Pages: 6
Enzymes are generally globular proteins, acting alone or in larger complexes. Like all proteins, enzymes are linear chains of amino acids that fold to produce a three-dimensional structure. The sequence of the amino acids specifies the structure which in turn determines the catalytic activity of the enzyme.[18] Although structure determines function, a novel enzyme's activity cannot yet be predicted from its structure alone.[19] Enzyme structures unfold (denature) when heated or exposed to chemical
Words: 336 - Pages: 2
Task 4: Metabolism 1. They speed up reactions and do not change themselves, which means they can be used over and over. Enzymes help facilitate chemical reactions. Enzymes will lower the activation energy needed to start the reaction and that is how the reaction will be sped up. Enzymes are specific for certain reactions and are proteins. Not all catalysts though are enzymes. (Sanders, 2014) 2. (Gresham HS IB Biology, 2007) 3. (Hudon-Miller, S. 2012) 4 & 5. When table sugar
Words: 756 - Pages: 4
