are often used to determine the identity of bacteria and differentiate between its species. There are numerous types of tests meant to detect key characteristics such as morphology, stain, motility, fermentation pathways used, oxygen requirements, enzymes present, and redox tests used. By conducting these tests with aseptic techniques, one can usually narrow down an unknown bacterium to its family; with the help of dichotomous keys and Bergey’s Manual of Determinative Bacteriology, particular genus
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test tubes in the test tube rack. Cut out circles from the 2 filter papers using a 6mm hole puncher. Obtain a 500mL bottle of 30% hydrogen peroxide, and measure 25.0 mL [±0.5mL] of Bos Taurus Liver Catalase Enzyme Solution (4.0 mol/L), with the use of a 50.0 mL graduated cylinder. Pour the 25.0 mL enzyme solution into a 250 mL beaker. Using the (cleaned) 50.0 mL graduated cylinder, measure 10.0 mL [±0.5mL] of hydrogen peroxide. Pour 10.0 mL of H2O2 into one of the 5 test tubes, with the use of a funnel
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the laboratories of respected universities such as The Ohio State University, LSU and the University of Colorado Denver. The results of these studies have been published in mainstream medical journals such as “Free Radical Biology & Medicine” and “Enzyme Research.” Below are summaries of these peer-reviewed studies: Antioxidants for the Treatment of Patients with Severe Angioproliferative Pulmonary Hypertension? Virginia Commonwealth University Antioxid Roedox Signal. 2012. Sept 28. Phytochemical
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for the identification of Escherichia coli. An enzyme is produced by E.coli which is beta D glucouronidase. Beta d glucouronidase in turn hydrolyzes beta d glucopyranosid uronic derivatives to aglycons and D glucuronic acid. Bile solubility test is used in laboratory for differentiation of alpha hemolytic Streptococci from Streptococcus pneumoniae. In catalase test it acts as a catalyst for the breakdown of hydrogen peroxide to oxygen and water. Catalase production test is done for an organism by bringing
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Introduction: Biological organisms are classified uniformly in order to easily categorize and identify organisms. This classification, or taxonomy, uses the genus name followed by the species name, in Latin. By having a universal method of identifying bacteria allows for all scientists from any part of the world to identify the same species in an identical manner allowing for a precise of classification. Bacteria are distributed throughout the world in almost every conceivable habit. Bacteria
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for life, they can also be damaging; hence, plants and animals maintain complex systems of multiple types of antioxidants, such as glutathione, vitamin C, and vitamin E as well as enzymes such as catalase, superoxide dismutase and various peroxidases. Low levels of antioxidants, or inhibition of the antioxidant enzymes, cause oxidative stress and may damage or kill cells. As oxidative stress might be an important part of many human diseases, the use of antioxidants in pharmacology is intensively
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Able to precipitate proteins – Good fixative and preservative 3. ACTIVITY 1A /1B Subcellular components of the Living Cell• Sediment 1 – Nuclear Fraction – Nuclei and Unbroken Cells• Sediment 2 – Mitochondria• Sediment 3 – Microsome – Proteins, Enzymes, Inorganic Ions 4. ACTIVITY 1A /1B Subcellular components of the Living Cell• Qualitative Tests – Carbohydrates • Molisch Test • Benedict’s Test – Proteins • Biuret Test • Xanthoproteic Test – Lipids • Sudan Test • Acrolein Test 5. ACTIVITY 1A /1B
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carried out during the chithiraipattam (April-May) in India in 2010 to study the effects of foliar applications of different concentrations of seaweed liquid fertilizer (SLF) of Sargassum wightii on growth, biochemical constituents and antioxidant enzymes of Helianthus annuus L. to explore the possibilities of reducing or avoiding chemical fertilizers and to obtain the highest growth and productive parameters. The foliar spray was given twice in 15days interval at two concentrations (2.5%, 5.0%) of
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Toxicity Paper ENV/410 Toxicity Paper Chemicals that produce systemic toxicity do not always produce the same degree of toxicity in all organs. Instead, one or two organs are usually the target of most of the toxicity produced by the toxic chemical in question. These are the target organs for the toxicity for that chemical. The organ that is the target of toxicity most commonly involved in systemic effects is the central nervous system followed by skin, blood circulation system, liver, lungs
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Toxicity Paper Chemicals that produce systemic toxicity do not always produce the same degree of toxicity in all organs. Instead, one or two organs are usually the target of most of the toxicity produced by the toxic chemical in question. These are the target organs for the toxicity for that chemical. The organ that is the target of toxicity most commonly involved in systemic effects is the central nervous system followed by skin, blood circulation system, liver, lungs and kidneys. Muscle and
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