The premise of this lab is to measure our enzymatic activity of Alkaline phosphatase, we began measuring our activity by applying different substrate concentration of p-nitrophenyl phosphate in relationship to absorbance over the span of 70 seconds, the data obtained in table 5.1, the different substrate concentration 0.9mM, 0.675mM, 0.45mM, 0.225mM, 0.09mM, 0.045mM, 0.0225mM. keeping our enzyme constant at 100μL, what we concluded is a significant decrease on the absorbance as we decrease the substrate
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hydrogen peroxide, and dish soap. You need a light to see where you’re going and to search for food. But, I wonder if a store bought glow stick would last longer? In a bought glow stick the mixtures are sensitizer is a chemical that causes an allergic reaction in the normal tissue after exposure to the chemical. Fluorophore is a molecule creating fluorescent emission in the visible light spectrum. Chromophores are wavelengths of light that are absorbed by fluorophores, creating visible light. Bought glow
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The purpose of the hydrate lab was to calculate the percent mass and the number of moles of H2O within a sample of Copper (II) Sulfate Pentahydrate. During the process of the experiment, there were multiple errors that lead to an average of 14% error from the theoretical value. One of the errors included the duration of time in which the hydrate was placed on top of the bunsen burner flame. The alterations in the duration of time between the two trials would lead to a difference in the amount of
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The purpose of today’s lab was to observe precipitation reaction and to create full and net ionic equations for each precipitate formed. The first part of this lab required us to combine four unknown solutions in well plates and see which react. Next, we had to create eight mixtures, in the well plates. Most of these mixtures created precipitates. The second part of the lab was essential in identifying which solution was in each of the unknown bottles. We were able to identify all four unknown solutions
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The purpose of this lab was to determine the effect of temperature and pH on the reaction rate of Catecholase. The substrates of catecholase are catechol and oxygen, and it is an enzyme that speeds up chemical processes. Enzymes work best under optimal conditions, and we tested the reaction rate of catecholase with substances containing different temperatures and pH levels. pH measures the acidity or alkalinity of a substance, and is measured on a scale from 1 to 14. Temperature is the measurement
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In the 4th quarter of 9th grade science we continued our study of chemistry with a focus on chemical formulas, chemical reactions, and stoichiometry. Formative assessments included practice questions on how to write proper names and formulas for chemical compounds, balancing equations, converting between chemical units, and titration problems. The lab experiments for the quarter consisted of a freezing point experiment, chemical flame test comparison, the activity series of four metals, production
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Mystery Substance Lab Write-Up The haters always say, “Johnny, you’re bad at chemistry.” Well, this lab proved the haters wrong. Charlie and I were the first in our class to guess the mystery substance correctly, (we hope), and we did it while doing the fewest tests (we think). “That’s a weird brag,” the haters would say. I think they’re just jealous. The goal of this lab was to find out as much as possible about the given mystery substance. Charlie and I did that, and more. As I said earlier, we
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act as efficient catalysts for specific chemical reactions. They are able to convert a specific set of reactants, known as substrates, into a specific set of products. Even at low temperatures, enzymes continue to allow a reaction to occur by lowering the activation energy of the given reaction. Reactions continue to occur even in the absence of enzymes, however due to the slow reaction rates without enzymes, sometimes the effects of the reaction would be considered insignificant. Enzymes are present
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Introduction: This unit, about Enzymes and Biotechnology, contains information on how enzymes work in chemical reaction. For every enzyme there is a substrate made of reactant molecules that is worked upon. During a chemical reaction, the enzyme lactase breaks lactose molecules into their monomers, glucose and galactose. This lab is observing enzyme specificity as demonstrated by how lactase affects the presence of glucose in soy, cow, and rice milk. Data: Conclusion: 1. Cow milk contains lactose
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This is a lab in which we will be using combustion to make magnesium and turn into a fine powder. We will first put the crucible on a mesh above bunser burner to heat it up to get out any moisture. We will then record the weight and weigh the magnesium in the crucible. Then we will put the crucible over a flame with the magnesium inside until it turns into the powder. There are many changes in the world. These changes are like changes in chemistry because some you don’t see and there is some
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