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Effects of Surfactants

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Submitted By dewble
Words 5669
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1611

9002 ,61 yluJ detpeccA ;9002 ,31 yaM desiveR ;9002 ,91 hcraM devieceR rk.er.irfk@miktc :sserdda liam-E 7529-087-13-28+ :xaF ;8319-087-13-28+ :leT :rohtua gnidnopserroC*

,revewoH .)9( snoitidda tnenopmoc fo redro dna dohtem noitaraperp eht no sdneped noislumeonan a fo ytilibats ehT .)8( mn 001 naht ssel sezis telpord gnivah selucelom tnatcafrus fo mlif laicafretni na yb dezilibats retaw dna lio fo snoisrepsid tnerapsnart dna elbats yllacimanydomreht era snoislumeonaN .)7,6( sdnuopmoc larutan rehto dna ,slarenim ,nimativ ,stnadixoitna ,sloretsotyhp ,sdica yttaf detarutasnuylop ,sdionetorac sa hcus stneidergni evitcaoib ynam fo ytilibaliavaoib dna ytilibulos eht evorpmi ot laitnetop eht sreffo hcihw ,metsys yreviled noislumeonan eht si seigolonhcetonan gnisimorp tsom eht fo enO .sdioniciaspac lareves dna ,slairetam deroloc ,sexaw ,slio laitnesse fo erutxim xelpmoc a sniatnoc dna smucispac fo stiurf epir deird eht fo noitcartxe lairtsudni eht si )CO( mucispac niseroelO .)5( reppep toh fo stnenopmoc tnegnup eht pu ekam hcihw ,sdioniciaspac gnirrucco yllarutan fo ylimaf eht fo srebmem owt era )edimananon -6-non-lyhtem-8-lyhtem]lynehp-yxohtem-3-yxordyh-4[-N( niciaspacordyhid dna )edimane-6-non-lyhtem-8-lyhtem]lynehp -yxohtem-3-yxordyh-4[-N( niciaspaC .)4-2( snoitacilppa lacidem ni dezilitu si dna evitidda doof dna ecips a sa demusnoc ylediw si niciaspaC .)1( yticixot fo ecnesba eht ni ytivitca tnadixoitna sti ot eud ,sredrosid evitarenegedoruen dna ,ekorts ,noisnetrepyh ,esaesid ralucsavoidrac ,recnac sa hcus sesaesid cinorhc detaidem-sserts evitadixo tneverp nac ti esuaceb tneidergni lanoitcnuf lufesu a sa nwonk si )reppep toh ni tneidergni tnegnup niam eht( niciaspaC noitcudortnI stneidergni lanoitcnufoib rof noitaluspacneonan no desucof ylralucitrap neeb sah ygolonhcetonan doof ,yltneceR .snoislumeorcim W/O dna O/W htob fo noitamrof eht depleh )locylg enelyporp ro lorecylg( sloylop dna )lonahte sa hcus( slohocla niahc-trohs fo snoitidda taht dnuof saw tI .)32( slohocla niahc-trohs dna sloylop fo snoitidda yb dna stnatcafrus cinoinon elbatius gnisu detalumrof eb osla nac snoislumeorcim W/O edarg-dooF .snoitacilppa lacituecamrahp dna ,citemsoc ,doof ni desu era taht stnatcafrus evisnepxeni yllaicremmoc ,evitcartta yllaicepse era sneewT ,stnatcafrus cinoinon eht lla fO .sretse ragus dna )sneewT( sretse natibros detalyxohte sa hcus stnatcafrus edarg-doof no desab smetsys sa tsixe selpmaxe wef yreV .)22( erutcurts W/O na otni trevni lliw ,noitulid rehtruf nopu ,yllautneve taht serutcurts suounitnocib mrof dna retaw htiw detardyh eb nac noitisopmoc noitulid niatrec a ta esahp lio eht ni demrof era taht serutcurts delbmessa -fles ehT .noitatiga rednu aidem suoeuqa otni decudortni nehw snoislume )W/O( retaw-ni-lio enif mrof esehT .tnatcafrus-oc dna ,tnatcafrus ,lio fo erutxim ciportosi na htiw dehsilpmocca eb nac noitacifislume ylbmessa-fleS .)12-81( sdohtem erutarepmet noisrevni esahp dna ,)71,61( noitisnart esahp ,noitacifislume suoenatnops ,)51-31( noitacifislume ylbmessa-fles edulcni seuqinhcet ygrene wol esehT .selcitrap dezis-onan fo noitamrof eht etomorp ot seitreporp dna roivaheb esahp fo egatnavda gnikat yb depoleved neeb evah )21( sdohtem noitacifislume ygrene-wol ,revoeroM .snoislumeonan eraperp ot desu eb nac noitazinegomoh cinosartlu dna ,noitazinegomoh erusserp-hgih ,gnirrits raehs -hgih sa hcus sdohtem noitacifislume ygrene-hgiH .)11,01( snoislume dionetorac gniraperp rof ygolonhcet siht fo esu eht erolpxe taht snoitacilbup wef a ylno era ereht dna ,snoitacilppa lairtsudni rieht ni detimil yrev neeb evah yeht

mucispac niseroelo ,metsys yreviled evitcaoib ,tnatcafrus ,ylbmessa-fles ,noislumeonan doof :sdrowyeK .mn 29 ot 56 morf gnignar ,egarots gnirud sezis laitini rieht deniatniam snoislumeonan eht ,yllaniF .mn 001 ot 51 morf gnignar sezis elcitrap ni detluser ,MS+retaw/08 neewT/CO dna ,GP+retaw/08 neewT/CO ,retaw/08 neewT/CO fo smetsys gnisu noitacinosartlu yb deraperp snoislumeonan CO ehT .noislume eht fo ezis elcitrap eht no tnedneped erew taht noitarapes esahp dna noiger yduolc elpitlum a htiw ,detneserp erew snoislumeonan esahp-enO .snoislume eht fo sezis eht gnirusaem yb smargaid esahp gnisu denimreted erew saera noislumeonan eht dna ,ylbmessa-fles dna noitacinosartlu htob yb deraperp erew snoislumeonan ehT .CO yfislume ot stnatcafrus suoirav eht fo ytiliba eht dehsiugnitsid ylraelc seicneiciffe noitacifislume denimretred eht dna ,smetsys esahp-eno sa snoisrepsid elbats htiw snoislumeonan CO decudorp 08 neewT .selcihev doof sa esu ot detalumrof erew serutxim gnidnopserroc rieht dna ,)MS( etaraetsonom esorcus ,)GP( locylg enelyporp ,)08 dna ,06 ,04 ,02 neewT( sretse natibros enelyhtyxoylop ,)CO( mucispac niseroelo dna retaw fo gnitsisnoc smetsys noislumeonan dooF :tcartsbA aeroK ,947-021 luoeS ,ytisrevinU iesnoY ,ygolonhcetoiB fo tnemtrapeD2 aeroK ,378-963 kubgnuhC ,gnoesmuE ,noitartsinimdA tnempoleveD laruR ,ecneicS labreH & erutlucitroH fo etutitsnI lanoitaN ,hcraeseR porC labreH fo tnemtrapeD1 aeroK ,647-364 iggnoeyG ,mangnoeS ,etutitsnI hcraeseR dooF aeroK ,retneC hcraeseR onaN-oiB

snoislumeonaN dedaol -niciaspaC fo ytilibatS dna noitamroF eht no stnatcafruS fo stceffE ygolonhceT dna ecneicS dooF fo yteicoS naeroK ehT

*miK iaT-gnohC dna , gnawH nawK-eaJ ,ohC niJ-gnoY ,miK niJ-luhC , iohC niJ-eA
2 1



)9002( 2711 ~ 1611 .pp ,5 .oN ,81 .loV .lonhcetoiB .icS dooF

elcitrap eht ,syad 82 rof snoislume eht gnirots retfA .)1 .giF( retaw dellitsid htiw detulid neht dna moor krad a ni derots dna deretlif erew selpmas ehT .)ASU ,AP ,cifitneicS rehsiF ;005 ledoM ,rotarbmemsid cinoS( rotacinosartlu na gnisu nim 3 rof edutilpma %64 ta detacinos neht dna nim 1 rof Co05 ta detaeh saw esahp suoeuqa dna ,tnatcafrus ,CO gniniatnoc erutxim-erp ehT .erutxim mrofinu a evig ot nim 3 rof ylsuoenegomoh derrits dna soitar tnereffid ta dexim erew 08 neewT dna CO ehT .ylevitcepser ,oitar thgiew tnatsnoc a ta )3 : 001( MS + retaw ro )1 : 2( GP + retaw ro 08 neewT dna ,retaw ,CO htiw deraperp erew stnenopmoc 3 fo gnitsisnoc serutxim-erp eht :rennam gniwollof eht ni detcurtsnoc erew smargaid esahP .snoislumeonan eb ot deredisnoc erew skeew 4 retfa esahp-eno tnerapsnart a deniatniam taht selpmas ehT .tnenopmoc hcae fo thgiew eht no desab tnatcafrus-oc dna ,tnatcafrus ,CO fo snoitisopmoc gniyrav htiw deraperp erew serutxim-erP

noitacinosartlu yb snoislumeonan CO fo noitaraperP

.)ASU ,AM ,drofliM( sretaW morf desahcrup saw retaw dellitsid dezinoieD .edarg lacitylana fo erew stnegaer rehto llA .)dnalreztiwS ,GS shcuB ,53 : 56 = niciaspacordyhid:niciaspac( akulF morf desahcrup erew sdradnats )CLPH( yhpargotamorhc diuqil ecnamrofrep hgih rof sdioniciaspaC .hcirdlA -amgiS morf desahcrup saw )GP( locylg enelyporpylop dna ,)aeroK ,luoeS( slleW nihslI yb deilppus saw )061 -F sa elbaliava yllaicremmoc ;MS( etaraetsonom esorcuS .)ASU ,OM ,siuoL .tS( hcirdlA-amgiS morf desahcrup erew )08 neewT( etaeloonom natibros enelyhteyxoylop dna ,)06 neewT( etaraetsonom natibros enelyhteyxoylop ,)04 neewT( etatimlaponom natibros enelyhteyxoylop ,)02 neewT( etarualonom natibros enelyhteyxoyloP .UHS 000,001 saw eerged ssentoh sti dna g/gm 60.0 ± 84.5 saw CO eht fo tnetnoc sdioniciaspac ehT .)aeroK ,luoeS( F&G morf deilppus saw )CO( mucispac niseroelO slairetaM sdohteM dna slairetaM

.mpp 001 dna ,05 ,03 ,01 :snoitartnecnoc gniwollof eht fo sdioniciaspac fo sdradnats laicremmoc morf deniatbo sevruc noitarbilac fo snaem yb deifitnauq erew niciaspacordyhid dna niciaspaC .mn 082 ta tes saw rotceted SIV/VU eht dna ,nim 03 rof nim/Lm 8.0 saw etar wolf ehT .)v/v( 03 : 07 fo oitar a ta retaw dezinoied dna lonahtem fo erutxim a saw esahp elibom ehT .metsys tneidarg a htiw )ASU ,AC ,aralC atnaS ,seigolonhceT tneligA ;mµ 5 ,mm 052 × 6 × 4 ,81C -BDX espilcE xabroZ( nmuloc 81C a no demrofrep saw noitarapeS .)aeroK ,luoeS ,ygolonhceT nilluY ;MTorhCitluM( noitats atad a dna ,)napaJ ,oykoT ,.dtL scitpO amoS ;0473 -S( rotceted SIV/VU a ,)napaJ ,oykoT ,noitaroproC okieS revE ;106 rellortnoc tneidarG( pmup a fo detsisnoc metsys CLPH ehT .)72( . sivaD fo erudecorp deifidom a gnisu CLPH yb deifitnauq erew mucispac niseroelo eht ni sdioniciaspac fo stnuoma ehT CO fo tnetnoc dioniciaspaC la te

.egarots gnirud snoislume eht fo sezis elcitrap eht gnirusaem yb detaulave saw snoislumeonan eht fo ytilibats ehT .smargaid esahp yranret yb detagitsevni erew snoislumeonan eht fo noitamrof eht no snoitartnecnoc dna sepyt tnatcafrus eht fo stceffe ehT .locylg enelyporpylop dna ,etaraetsonom esorcus ,)08 dna ,06 ,04 ,02 neewT( sdica yttaf fo sretse natibros enelyhteyxoylop sa hcus stnatcafrus fo sepyt tnereffid gnisu ylbmessa-fles dna noitacinosartlu yb deraperp erew mucispac niseroelo gniniatnoc snoislumeonan W/O ,hcraeser siht nI .)62( ytilibaliavaoib dna ,ytilibats ,ytilibulos sa hcus seitilanoitcnuf edivorp dna ezis ni mn 005-02 fo smetsys tnerapsnart era hcihw ,serutcurts dezis-retemonan ni detaluspacne eb nac tneidergni evitcaoib A .)52,42( detroper neeb evah snoislumeonan fo snoitacilppa lufsseccus dna ,lortarevser dna niciaspac sa hcus

.etaraetsonom esorcus ,MS ;locylg enelyporp ,GP ;mucispac niseroelo ,CO .ylbmessa-fles dna noitacinosartlu yb snoislumeonan fo noitaraperP .1 .giF

.la te iohC .J- .A

2611

latigiD dna ,)ASU ,CD ,notgnihsaW,.dtL nacsoiB ;NATAG ,297 ledoM( aremac nacSoiB dirg mm 3 acilper gnitarg a htiw detarbilac erew selacs eht dna Vk 021 ta deriuqca erew segamI .)sdnalrehteN ehT ,nevohdniE ,spillihP ;21 IANCET( epocsorcim nortcele noissimsnart a yb denimaxe neht erew sdirg ehT .deird ria neht dna ,niaga retaw htiw dehsaw ,retaw erupartlu ni etateca lynaru %2 fo Lµ 7 htiw deniats erew selpmas eht yletaidemmI .retaw erupartlu htiw ecno dehsaw dna sdirg reppoc detaoc-nobrac no decalp erew snoislumeonan delaenna eseht fo Lµ 7 ,gniniats evitagen roF )MET( ypocsorcim nortcele noissimsnarT .)ASU ,AP ,ellivyremogtnoM ,.cnI cartorciM( 052 APN cartonaN a gnisu gnirettacs thgil resal cimanyd elgna-wol yb denimreted erew snoislumeonan eht fo noitubirtsid ezis dna ezis elcitrap ehT noitubirtsid ezis elcitrap fo noitanimreteD .)1 .giF( sisylana erofeb rh 42 rof etarbiliuqe ot dewolla dna deretlif erew snoislumeonan eht ,yllaniF .noislumeonan eht gnimrof ,rh 2 rof gnirrits citengam etaredom rednu )Lm 001( esahp suoeuqa htiw detulid dna dehgiew yletarucca saw )g 5.0( erutxim ciportosi ehT .noitamrof noislumeonan

rof smargaid esahp eht fo yduts deliated rof CO ot tcepser htiw tnatcafrus fo snoitartnecnoc gnisaercni tcelfer ot nesohc erew soitar erutxim esehT .2 elbaT ni detacidni sa )5:1 dna ,4:1 ,3:1 ,2:1 ,1:1( soitar thgiew tnereffid ta dexim erew 08 neewT dna CO ehT .)82,72( erudecorp debircsed ylfeirb gniwollof eht ot gnidrocca noitacifislume ylbmessa-fles deifidom yb deraperp erew snoislumeonan ehT ylbmessa-fles yb snoislumeonan CO fo noitaraperP .snoislumeonan eht fo noitaraperp eht rof detceles erew tnatcafrus-oc dna tnatcafrus elbatius a neht dna ,detset-erp erew tnatcafrus fo snoitisopmoc dna sepyt gniyrav htiw serutxim yranreT .noislumeonan W/O elbats a niatbo ot detset erew lonahte dna ,GP ,MS ,08 neewT ,06 neewT ,04 neewT ,02 neewT sa hcus ,stnatcafrus-oc dna stnatcafrus cilihpordyh suoiraV .)1 elbaT( smargaid esahp eht rof snoitanibmoc tnereffid 3 ni depuorg erew tnatcafrus dna ,retaw ,CO ehT snoislumeonan rof stnatcafrus fo gnineercS .dnuof erew smetsys esahp-eno ,tnerapsnart hcihw ta stniop eht tneserper ot sisab w/w a no detcurtsnoc erew smetsys tnenopmoc-3 eht fo smargaid esahp-yranret ,yllaniF .denimreted erew snoislumeonan eht fo sezis
2 1 0 2 1 0 2 1 0 HOtE oitar gnixiM

08 neewT

06 neewT

× × × × × × ○ × × × × × × × × △ × × × × × × × × × × × tnerapsnarT ○ ○ ○ ○ ○ ○ × ○ ○ ○ ○ ○ ○ ○ ○ △ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○

○ ○ ○ ○ ○ ○ × △ ○ ○ ○ ○ ○ ○ ○ × △ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○

○ ○ ○ ○ ○ ○ × △ ○ ○ ○ ○ ○ ○ ○ × △ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○

○ ○ ○ ○ ○ ○ △ △ △ ○ ○ ○ ○ ○ ○ △ △ △ △ △ △ △ △ × × × × desrepsiD 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 CO

3 2 1 3 2 1 3 2 1 3 2 1 3 2 1 3 2 1 3 2 1 3 2 1 3 2 1

02 neewT

snoislumeonan fo noitamrof eht no sneewT dna ,lohocla lyhte ,)CO( mucispac niseroelo fo soitar gnixim eht fo stceffE .1 elbaT
3611

detarapes euqapO & pmulC detaluccolF noitamrof noisumeonan fo ecnaraeppA

neewT

snoislumeonaN dedaol-niciaspaC fo ytilibatS dna noitamroF

.)100.0 < p dna ,10.0 < p ,50.0 < p( tnereffid yltnacifingis erew seulav eht fi enimreted ot deyolpme erew tset egnar elpitlum s’nacnuD dna ecnairav fo sisylanA .)ASU ,CN ,yraC ,smetsyS sisylanA lacitsitatS( 8V swodniW rof SAS gnisu yllacitsitats dezylana erew )3 = n( stnemerusaem eht fo stluser ehT sisylana ataD .skeew 4 rof Co52 ta egarots gnirud ,).cnI cartorciM( 052 APN cartonaN a gnisu gnirettacs thgil resal cimanyd elgna wol yb ezis elcitrap eht gnirusaem yb detaulave saw snoislumeonan eht fo ytilibats ehT snoislumeonan fo ytilibatS .erutpac egami rof desu saw )4.3 v nataG( erawtfos hpargorciM
.selpmas etacilper 5 fo DS±naem sa desserpxe era seulav llA
)1

tibihxe ton did 02 neewT htiw deraperp snoislume ehT .snoislume fo ytilibats dna noitamrof eht ni neewT elbatius a fo noitceles eht rof ypocsorcam yb detaulave sa tset gnineercs eht swohs 1 elbaT .CO dna lohocla lyhte fo desopmoc serutxim htiw stnatcafrus sa desu erew sneewT tnereffid ,noitamrof noislume no sneewT fo seires a fo tceffe eht yfitnedi ot redro nI .snoislumeonan ro -orcim mrof yeht hcihw htiw esae eht dna ,laitnetop noitatirri ,yticixot wol ylevitaler rieht ot eud yliramirp ,smetsys noislume doof ni desu ylediw era sneewT ,eseht fO .setabrosylop dna ,sneewT ,sedirecylgonom gnidulcni ,yrtsudni doof eht ni desu ylnommoc era stnatcafrus cinoinoN

noitamrof noislume rof stnatcafrus fo gnineercS noissucsiD dna stluseR

5.1

1

0.1

1

7.0

1

5.0

1

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84.0±5.51 28.2±4.66 48.2±461 16.5±852 7.67±463 0.22±283 38.1±9.12 19.1±7.03 01.1±0.82 44.1±7.52 59.2±6.83 56.4±261 4.01±942 45.3±932 55.0±1.96 14.6±8.56 03.4±5.77 9.61±3.28 30.1±8.43 8.51±8.28 3.02±041 17.9±161 3.11±291 2.23±651 4.11±951 0.61±251 9.72±521 24.9±551 75.8±252 6.23±612 1.32±391 6.41±402 2.85±432 7.51±462 )mn( ezis elcitraP

noitacinosartlu yb stnetnoc retaw tnereffid ta esahp lio eht sa 08 neewT dna )CO( mucispac niseroelo no desab deraperp snoislumeonan fo sezis elcitraP .2 elbaT
.la te iohC .J- .A
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5.16 1.32 6.45 3.72 4.44 3.33 5.73 5.73 2.43 5.93 6.82 9.24 2.69 39.1 3.38 33.8 4.17 3.41 7.66 7.61 0.06 0.02 0.05 0.52 9.24 6.82 4.93 3.03 7.69 53.1 5.58 89.5 6.47 5.01 2.07 9.21 8.36 9.41 1.45 9.81 9.64 9.12 3.34 3.32 0.73 9.52 7.66 1.11 1.75 3.41 0.05 7.61 4.64 9.71 0.04 0.02 8.96 89.6 6.06 90.9 6.35 7.01 0.05 5.11 5.34 0.31 8.72 7.61 retaW 08 neewT )w/w ,%( noitisopmoC

3.51 2.81 2.22 0.52 3.62 6.82 29.1 33.8 3.41 7.61 0.02 0.52 6.82 3.03 39.1 55.8 9.41 5.71 3.12 0.72 3.13 3.33 0.73 2.22 6.82 3.33 7.53 0.04 3.32 3.03 7.53 5.83 5.34 6.55 CO

4 3 2 5.1 3.1 1 05 01 5 4 3 2 5.1 3.1 05 01 5 4 3 2 5.1 3.1 1 3 2 5.1 3.1 1 3 2 5.1 3.1 1 5.0 retaW

3.0

1

08 neewT oitar gnixiM

CO

.deifitnedi eb dluoc snoiger noislumeonan W/O eht taht os ,puorg hcae rof yletarapes detcurtsnoc erew smargaid esahp yranret ehT .smargaid esahp gnisu saera noislumeonan eht yduts ot )4-2 elbaT( spuorg 3 ni serutxim esahp lio-tnatcafrus sa desu erew GP dna ,MS ,08 neewT ,CO .)92( margaid esahp-yranret a fo dia eht htiw desserpxe eb nac noitisopmoc sti dna esahp lio-tnatcafrus a rof serutxim fo ruoivaheb esahp eht neewteb pihsnoitaler ehT noitacinosartlu yb noitamrof noislumeonaN .snoislumeonan CO fo seitreporp eht no ,noitacinosartlu dna ylbmessa gnidulcni ,sdohtem noitaraperp dna stnatcafrus-oc fo stceffe eht yduts ot stnemirepxe tneuqesbus ni desu saw 08 neewT ,eroferehT .CO yfislume ot stnatcafrus suoirav eht fo ytiliba eht dehsiugnitsid ylraelc seicneiciffe noitacifislume denimreted eht dna ,smetsys esahp-eno noisrepsid

elbats era taht snoislumeonan CO ecudorp ot tnatcafrus elbatius a sa desu eb nac 08 neewT taht detacidni stluser esehT .)11( noislume eht fo ezis elcitrap eht sesaerced tnatcafrus eht fo noitartnecnoc eht gnisaercni oslA .yltneiciffe erom noislume W/O na ni selcitrap ezilibats dna parw nac yticilihpordyh retaerg htiw stnatcafruS .)1:0:3( CO:lohocla lyhte:neewT sa deraperp nehw lohocla lyhte tuohtiw metsys esahp tnerapsnart a deniatniam noislume eht ,ralucitrap nI .ytreporp ycnerapsnart eht fo noitpecxe eht htiw ,CO dna lohocla lyhte fo desopmoc noitisopmoc hcae fo soitar lla ta ytisrepsid doog htiw snoislume decudorp osla ,08 neewT yleman ,desu stnatcafrus tnereffid 4 eht fo enO .nrettap detarapes dna ,depmulc ,detaluccolf a htiw deinapmocca dna tnerapsnart ton osla saw ti ,revewoh ;ytisrepsid doog ylevitaler a detibihxe taht noislume na decudorp 06 neewT .lohocla lyhte tuohtiw llew esrepsid ton dluoc snoislume eht ,ralucitrap ni ;snrettap detarapes dna ,depmulc ,detaluccolf dewohs dna ycnerapsnart
.selpmas etacilper 5 fo DS±naem sa desserpxe era seulav llA
)1

5.1

1

0.1

1

7.0

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5.0

1

4.44 0.14 4.63 6.74 4.44 0.04 3.33 5.46 0.75 8.94 8.64 6.24 0.63 3.13 9.82 7.42 7.46 0.85 3.15 5.84 4.44 1.83 3.33 0.13 7.62 5.64 4.04 7.53 3.33 0.92 5.81 retaW

2.22 5.02 2.81 8.32 2.22 0.02 7.61 2.23 5.82 9.42 0.42 3.12 0.81 6.51 4.41 4.21 4.23 0.92 6.52 2.42 2.22 1.91 7.61 5.51 3.31 3.32 2.02 9.71 7.61 5.41 62.9 GP )w/w ,%( noitisopmoC

0.02 1.32 3.72 3.41 7.61 0.02 0.52 53.1 89.5 5.01 9.21 9.41 9.81 9.12 3.32 9.52 79.0 53.4 96.7 90.9 1.11 3.41 7.61 9.71 0.02 89.6 90.9 7.01 5.11 0.31 7.61 08 neewT

3.31 4.51 2.81 3.41 7.61 0.02 0.52 49.1 65.8 9.41 5.71 3.12 0.72 3.13 3.33 0.73 49.1 07.8 4.51 2.81 2.22 6.82 3.33 7.53 0.04 3.32 3.03 7.53 5.83 5.34 6.55 CO

3.0

1

)1

14.1±4.36 39.0±1.07 2.22±042 47.0±4.61 35.0±1.52 64.4±2.85 691±252 30.9±8.26 61.6±2.35 44.0±2.73 79.2±5.53 60.1±4.53 13.8±2.66 5.52±612 77.8±1.88 34.7±042 73.3±4.29 02.9±201 55.5±9.78 45.6±9.98 23.3±8.85 04.4±7.77 58.2±411 66.3±751 67.8±831 1.41±131 7.41±631 77.7±051 1.11±841 78.9±451 7.36±133 )mn( ezis elcitraP

5 4 3 5 4 3 2 05 01 5 4 3 2 5.1 3.1 1 05 01 5 4 3 2 5.1 3.1 1 3 2 5.1 3.1 1 5.0 )1:2( GP:retaW

08 neewT oitar gnixiM

CO

noitacinosartlu yb )GP( locylg enelyporp dna retaw fo soitar tnereffid ta esahp lio eht sa 08 neewT dna )CO( mucispac niseroelo no desab deraperp snoislumeonan fo sezis elcitraP .3 elbaT
5611

snoislumeonaN dedaol-niciaspaC fo ytilibatS dna noitamroF

.noitartnecnoc wol dna noitisopmoc elpmis sti ot eud esu doof rof snoislumeonan gnitalumrof ni laicifeneb eb yam metsys siht ,stneidergni noislume fo ytefas dna tsoc eht htob fo smret ni ,eroferehT .stnevlos sa tca ot dnet hcihw ,CO eht ni sdica yttaf fo ecneserp eht ot eud ylbamuserp ,tnevlos ro tnatcafrus -oc a fo noitidda eht tuohtiw retaw/08 neewT/CO metsys eht morf demrof eb nac noislumeonan a taht etartsnomed ylraelc stluser esehT .margaid esahp eht fo srenroc 08 neewT dna CO eht htob ni derrucco hcihw ,noitarapes esahp fo noiger eht si aera yerg krad eht ,yllaniF .mn 004 dna 001 neewteb gnignar ,noislume eht fo noiger yduolc ,elpitlum eht si aera yerg ehT .mn 001 woleb gnignar noislumeonan eht fo noiger tnerapsnart eht ,esahp-eno eht si aera etihw ehT .noislumeonan eht fo ezis elcitrap eht gniziretcarahc yb denimreted saw yradnuob esahp ehT .2 .giF ni detneserp si retaw/08 neewT/CO metsys tnenopmoc -3 eht fo margaid esahp ehT .)01( revoc ot tnatcafrus erom eriuqer dluow hcihw ,saera ecafrus retaerg ylpmi sezis elcitrap rellams taht tcaf eht ot eud eb thgim sihT .tnatcafrus fo noitartnecnoc eht ot lanoitroporp ylesrevni saw noitubirtsid ezis elcitrap eht taht gnitacidni ,ezis elcitrap gnisaerced ot dael CO susrev tnatcafrus fo noitartnecnoc

eht gnisaercni taht raelc saw tI .)mn 84.0 ± 5.51( ezis elcitrap muminim fo noislumeonan a demrof )%5.16:%1.32 : %3.51( 4 : 5.1 : 1 fo oitar eht saerehw ,)mn 8.51 ± 8.28( ezis elcitrap mumixam fo noislumeonan a demrof ,CO fo tnuoma mumixam eht gniniatnoc ,)%1.45 : %9.81 : %0.72( retaw/08 neewT/CO metsys eht rof 2 : 7.0 : 1 fo oitar ehT .retaw %2.69-1.45 dna ,08 neewT %3.72-39.1 ,CO %0.72 -29.1 ta esahp suoeuqa eht ni mn 001 naht rellams erew sezis elcitrap eht ,retaw/08 neewT/CO fo metsys eht morf snoislumeonan gniraperp ni ,eroferehT .snoislumeonan fo noitamrof eht ni noitubirtsid ezis elcitrap tceffa yltaerg thgim 08 neewT fo noitartnecnoc eht taht detacidni stluser esehT .mn 001 naht rellams yltsom erew snoislumeonan eht fo sezis elcitrap eht ,5.1 : 1 fo oitar a ot dna ,1 : 1 ot 7.0 : 1 fo oitar a morf desaercni oitar 08 neewT:CO eht sa ,revewoH .mn 462 dna 521 neewteb gnignar sezis elcitrap fo snoislumeonan ni detluser 5.0:1 dna 3.0:1 fo soitar 08 neewT :CO eht taht nees eb nac tI .oitar thgiew CO tnatsnoc a ta dna retaw/C08 neewT fo soitar thgiew tnereffid ta noitartnecnoc 08 neewT eht fo noitcnuf a sa ,retaw /08 neewT/CO metsys eht morf deraperp snoislumeonan eht fo snoitubirtsid ezis elcitrap eht swohs 2 elbaT retaw /08 neewT/CO metsys eht no noitamrof noislumeonaN
.selpmas etacilper 5 fo DS±naem sa desserpxe era seulav llA
)1

5.1 1

1 1

7.0

1

5.0

1

8.95 0.35 7.46 3.85 5.84 9.39 0.38 5.27 1.86 0.26 5.25 5.54 3.49 4.48 7.47 6.07 7.46 5.55 5.84 1.54 6.49 9.58 1.77 3.37 7.76 retaW

97.1 95.1 49.1 57.1 64.1 28.2 94.2 71.2 40.2 68.1 75.1 73.1 38.2 35.2 42.2 21.2 49.1 66.1 64.1 53.1 48.2 05.2 13.2 02.2 30.2 MS )w/w ,%( noitisopmoC

1.32 3.72 7.61 0.02 0.52 53.1 89.5 5.01 9.21 9.41 9.81 9.12 79.0 53.4 96.7 90.9 1.11 3.41 7.61 9.71 85.0 56.2 67.4 66.5 89.6 08 neewT

4.51 2.81 7.61 0.02 0.52 39.1 55.8 9.41 5.71 3.12 0.72 3.13 49.1 07.8 4.51 2.81 2.22 6.82 3.33 7.53 59.1 58.8 9.51 9.81 3.32 CO

3.0

1

)1

55.0±7.33 54.1±8.35 36.1±1.72 59.0±6.43 94.9±321 71.0±08.6 40.0±7.71 12.1±2.52 16.3±9.13 97.6±0.53 47.8±2.66 69.9±531 72.0±37.4 99.2±0.61 1.81±4.95 7.91±3.09 4.93±521 2.23±8.99 9.11±4.88 74.3±961 47.3±7.34 99.1±4.24 43.4±1.99 5.04±551 5.53±841 )mn( ezis elcitraP

4 3 4 3 2 05 01 5 4 3 2 5.1 05 01 5 4 3 2 5.1 3.1 05 01 5 4 3 )3:001( MS:retaW

08 neewT oitar gnixiM

CO

noitacinosartlu yb )MS( etaraetsonom esorcus dna retaw fo soitar tnereffid ta esahp lio eht sa 08 neewT dna )CO( mucispac niseroelo no desab deraperp snoislumeonan fo sezis elcitraP .4 elbaT
.la te iohC .J- .A
6611

MS fo stceffe eht yduts oT MS+retaw/08 neewT /CO metsys eht no MS fo noitutitsbus laitrap fo tceffE

deraperp ew ,noitamrof noislumeonan CO no tnemecalper

.retaw + GP dna ,edirecylgirt niahc muidem ,nihticel fo desopmoc smetsys noislume ni noiger latsyrc diuqil eht desaercni dna noiger noislumeonan esahp -eno ,raelc eht fo tnetxe eht decuder yltnacifingis retaw % tw 01 sa elttil sa neve ,retaw htiw GP gnicalper nehw taht dnuof ohw )03( letaP fo hcraeser eht htiw detalerroc stluser esehT .08 neewT eht gnicalper fo tceffe eht dewohs hcihw ,%03 woleb saw 08 neewT fo tnuoma eht nehw demrof erew sesahp-elpitlum dna eno eht htob ,noitidda nI .retaw/08 neewT/CO metsys eht naht noiger rellams ylthgils a detibihxe metsys GP+retaw/08 neewT/CO eht taht dewohs hcihw ,sesahp-elpitlum dna -eno eht fo stnetxe eht ni ecnereffid egral a saw ereht ,metsys eht ni retaw eht ecalper ot GP fo noitidda eht yB .retaw/08 neewT/CO metsys eht gnisu devresbo esoht ot ralimis erew noiger noitarapes eht dna ecnetsixe noislumeonan fo tnetxe ehT
.la te

.MS+retaw/08 neewT/CO metsys eht htiw noitacinosartlu yb demrof snoislumeonan fo margaid esahp-yranreT .4 .giF

.etaraetsonom esorcus ,MS ;08 neewT ,T ;mucispac niseroelo ,CO

.ylevitcepser ,noitarapes esahp fo noiger eht dna ;)mn 004 dna 001 neewteb( noiger yduolc ,elpitlum eht ;noiger )mn 001 woleb( noislumeonan esahp-eno eht tibihxe saera yerg krad dna ,yerg ,etihw ehT .3 .giF ni nwohs era smetsys GP + retaw/08 neewT/CO eht fo segnahc margaid esahp ehT .metsys eht ni GP+retaw %1.79-3.34 dna ,08 neewT %3.32-79.0 ,CO %3.33-49.1 htiw demrof erew snoislumeonan CO ,yltneuqesnoC .retaw/08 neewT/CO fo metsys eht ni %0.72 ot derapmoc sa %3.33 ta dehcaer yllamixam saw noislumeonan eht ni CO fo tnuoma eht ,metsys siht nI .mn 47.0 ± 4.61 fo ezis elcitrap muminim a htiw noislumeonan a demrof )%4.17 : %3.41 : %3.41( 5 : 1 : 1 fo oitar eht saerehw ,mn 77.8 ± 1.88 fo ezis elcitrap mumixam a htiw noislumeonan a demrof ,CO fo tnuoma mumixam a gniniatnoc ,)%3.34 : %3.32 : %3.33( 3.1 : 7.0 : 1 fo oitar GP + retaw : 08 neewT:CO ehT .retaw/08 neewT/CO fo metsys eht ot ralimis eb ot demees hcihw ,tnatcafrus fo tnuoma dlof 3-2 muminim a deriuqer esahp suoeuqa eht fo noitartnecnoc ehT .tnatcafrus-oc retaw/08 neewT /CO fo metsys eht ot derapmoc sa noiger noislumeonan eht ni sesaercni llams erew ereht GP + retaw/08 neewT/CO fo metsys eht nI .mn 001 woleb gnignar sezis elcitrap fo snoislumeonan ni detluser 5.1:1 dna ,1:1 ,7.0:1 ,5.0:1 fo soitar 08 neewT : CO eht taht nees eb nac tI .)3 elbaT( ylevitcepser ,tnevlos dna tnatcafrus eht sa GP dna 08 neewT htiw deraperp erew )1 : 2( GP + retaw/08 neewT/CO fo metsys eht rof snoitalumrof ehT .noiger noislumeonan esahp-eno ,raelc a gnicudorp osla elihw 08 neewT dna GP fo esu eht ecuder ot syaw dnif ot deen a si ereht ,eroferehT .)03( )gk /g 1 si doof ni timil eht( sesoprup doof rof seititnauq egral ni elbarovafnu si GP ,revewoH .tnatsnoc cirtceleid hgih ylevitaler a sah dna sdnob negordyh smrof ti esuaceb ,retaw ot ralimis si GP ,sloylop eht lla fO .snoitalumrof doof yek morf meht evomer ot erusserp tnatsnoc si ereht seirtnuoc ynam nI .egami evitagen a htiw stnatcafrus edarg doof era sneewT .stnenopmoc laudividni sa naht rehtar ,)GP ro lorecylg( sloylop dna ,)lonahte sa hcus( slohocla niahc-trohs ,stnatcafrus fo sepyt rehto htiw snoitanibmoc ni desu netfo era stnatcafrus ,yrtsudni doof eht ni snoislume fo seitreporp lanoitcnuf eht evorpmi ot redro nI GP+retaw/08 neewT /CO metsys eht no GP fo noitutitsbus laitrap fo tceffE
,CO .retaw/08 neewT/CO metsys eht htiw noitacinosartlu yb demrof snoislumeonan fo margaid esahp-yranreT .2 .giF .detarapes esahp ,SP ;08 neewT ,T ;mucispac niseroelo

.detarapes esahp ,SP ;locylg enelyporp ,GP ;08 neewT ,T ;mucispac niseroelo ,CO

.GP+retaw/08 neewT/CO metsys eht htiw noitacinosartlu yb demrof snoislumeonan fo margaid esahp-yranreT .3 .giF

7611

snoislumeonaN dedaol-niciaspaC fo ytilibatS dna noitamroF

deriuqer serutavruc tnereffid pu ekat ot ytilibixelf tneiciffus mlif laicafretni eht swolla dna ecafretni eht fo sserts gnidneb eht sesaerced stnatcafrus-oc fo ecneserp ehT .esahp suoeuqa eht morf detarapes saw esahp lio eht dna mn 003 revo fo egnar ezis a htiw demrof saw noislumeonan a ,)01 : 2 : 1( erutxim retaw/08 neewT/CO eht fo oitar eht gnisaerced yb ,revewoH .mn 50.2±3.61 fo ezis elcitrap a htiw noislumeonan a demrof ,tnatcafrus fo tnuoma muminim a gniniatnoc ,)%2.59:%75.3:%91.1( 01:3:1 fo oitar retaw/08 neewT/CO ehT .)5 .giF( tnetnoc retaw %7.58 naht erom fo noitidnoc eht rednu demrof saw noiger noislumeonan ehT .)5 elbaT( ylevitcepser ,%5.99-7.58 dna ,09.11 -73.0 ,68.2-80.0 fo segnar ta metsys retaw/08 neewT/CO eht htiw deniatbo erew snoislumeonaN .noitacinosartlu yb deraperp noislumeonan eht naht decuder erom saw ti dna ,ylbmessa-fles yb deraperp noislumeonan eht ni mn 001 woleb demrof noiger noislumeonan ehT .noitacinosartlu yb deraperp CO gniniatnoc noislumeonan W/O na rof snoitidnoc mumitpo eht tceles ot desu saw hcihw ,enola 08 neewT gnisu deraperp saw ylbmessa-fles yb noislumeonan ehT ylbmessa-fles yb noitamrof noislumeonaN .)33( irafaJ yb detroper esoht htiw tnemeerga doog ni era stluser esehT .tnevlos ralop evitanretla na htiw retaw fo tnemecalper eht nopu detcepxe eb dluoc roivaheb esahp fo ecneuqes emas eht rehtehw dehsilbatse
.la te

ylmrif neeb ton sah ti ,revewoh ,ylgnitseretnI .tnatcafrus -oc a tuohtiw metsys retaw/08 neewT/CO eht ni 08 neewT fo taht morf tnereffid ton erew metsys MS + retaw /08 neewT/CO eht ni MS dna 08 neewT fo stnetnoc ehT .)23( noitamrof noislume gnirud ytilibulos esol ton seod dna esahp lio eht otni noitulossid rof cilihpordyh erom si MS ,elihwnaeM .)13( esahp lio eht ni elbulosni yllacitcarp tub retaw ni elbulos era hcihw ,sloylop elpmis cilihpordyh tsael eht fo eno si GP taht tcaf eht ot eud eb yam tluser sihT .CO gniniatnoc noislumeonan W/O na fo noitamrof eht rof GP naht evitceffe erom saw tnatcafrus-oc a sa MS .mn 9.11 ± 4.88 fo ezis elcitrap mumixam a htiw noislumeonan a demrof ,CO fo tnuoma mumixam a gniniatnoc ,)%0.05 : %7.61 : %3.33( 5.1 : 5.0 : 1 fo oitar MS + retaw dna ,08 neewT ,CO ehT .noitamrof noislumeonan ni evitceffe erom saw MS dna ,metsys GP + retaw/08 neewT/CO eht ni GP fo taht ot derapmoc sa ,%48.2-64.1 ta ,llams ylemertxe saw esahp suoeuqa eht ni MS fo tnetnoc ehT .)4 .giF( metsys GP + retaw/08 neewT /CO dna metsys retaw/08 neewT/CO eht ot derapmoc sa noiger noislumeonan eht ni sesaercni llams erew ereht ,metsys MS + retaw/08 neewT/CO eht nI .)4 elbaT( mn 001 woleb gnignar sezis elcitrap fo snoislumeonan ni detluser 5.1 : 1 dna ,1 : 1 ,7.0 : 1 ,5.0 : 1 ,3.0 : 1 fo soitar 08 neewT:CO eht taht nees eb nac tI .)3 : 001( MS + retaw dna ,08 neewT ,CO fo erutxim a htiw esahp suoeuqa suonegomoh a
.selpmas etacilper 5 fo DS±naem sa desserpxe era seulav llA
)1

5

1

4

1

3

1

70.0±6.21 60.0±1.31 70.0±1.31 01.0±6.31 61.0±8.41 20.0±4.51 36.2±5.11 12.0±6.31 70.0±6.31 51.0±5.41 30.0±8.51 52.0±6.61 0521±5931 7.08±7.59 50.2±3.61 91.1±2.51 51.0±6.61 53.0±4.71 esahp-2 esahp-2 0616±1611 0352±0551 521±033 286±358 )mn( ezis elcitraP
)1

ylbmessa-fles yb stnetnoc retaw tnereffid ta esahp lio eht sa 08 neewT dna )CO( mucispac niseroelo no desab deraperp snoislumeonan fo sezis elcitraP .5 elbaT
.la te iohC .J- .A
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7.58 09.11 9.09 85.7 2.59 79.3 6.79 30.2 0.99 38.0 5.99 14.0 7.58 34.11 9.09 72.7 2.59 18.3 6.79 59.1 0.99 97.0 5.99 04.0 7.58 7.01 9.09 28.6 2.59 75.3 6.79 38.1 0.99 47.0 5.99 73.0 7.58 25.9 9.09 60.6 2.59 71.3 6.79 36.1 0.99 66.0 5.99 33.0 retaW 08 neewT )w/w ,%( noitisopmoC

83.2 25.1 97.0 14.0 71.0 80.0 68.2 28.1 59.0 94.0 02.0 01.0 75.3 72.2 91.1 16.0 52.0 21.0 67.4 30.3 95.1 18.0 33.0 71.0 CO

3 5 01 02 05 001 3 5 01 02 05 001 3 5 01 02 05 001 3 5 01 02 05 001 retaW

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