...Microbiology is requires not only an academic understanding of the microscopic world but also a practical understanding of lab techniques and procedures used to identify, control, and manipulate microorganisms. The proper identification of a microorganism is not only important in a microbiology lab but also in the medical, industrial, and pharmaceutical fields. In this lab report, lab techniques and procedures learned during this course were performed to assess each students’ practical knowledge in microbiology. 6In area of fields I mention earlier microbiology is very important to our vaccination and antibiotics we are using, understand that microorganism play a key role in maintaining life on earth, fixing gases and breaking down dead plant and animal matter into simpler substances that are used at the beginning of the food chain 6. Biotechnologists can also exploit the activities of microbes to benefit humans, such as in the production of medicines, enzymes and food. The goal of this lab report is 1) to demonstrate comprehension of the methods and lab techniques learned during the semester 2) to explain the tests performed on each isolated unknown that led to the identification of each unknown 3) and to give a background on the characteristics, pathogenicity and some uses of one of the identified unknowns. II. Introduction In this lab report I will discuss how I came to find my two unknown bacteria. Each bacteria have undergo many different test to eventually identifying...
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...Introduction: The microbiology of food and the environment are two very important fields in the large scope of microbiological research. Because microorganisms exist almost everywhere, it is important to determine the influences that they place on the food we depend on for survival, and the environment in which we humans call home. In this lab, we conducted five experiments in these two fields, and in doing so gained a better understanding of the influences and importance of microbes in food and the environment. The first exercise was the enumeration of soil microbes. This experiment showcased the immense diversity of bacteria, actinomycetes, and fungi found in soil. This diversity ranges from microbes that are beneficial to the environment by decomposing dead organic matter into energy sources usable by other organisms, to the pathogenic bacterial and fungal spores that can infect humans and animals alike. The techniques used are serial dilutions, which allow for quantification and a close estimation of the amount of said organisms found in a soil sample. (1) The second exercise that we conducted was the microbiology of water experiment. This is a very important standardized experiment used to determine the density of coliforms found in a 100 mL sample of water. It also can be used more specifically to determine the density of Escherichia coli, which can cause food poisoning amongst other illnesses. The techniques used are the multiple tube fermentation method, which involves...
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...HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased, not the writing. The purpose of scientific writing is not to entertain, but to inform. The writing should be simple and easy to understand. There is a specific style that must be followed when writing scientific reports. Scientific writing is typically written in the passive voice. The pronouns "I", "We" and "They" are not typically used.. For example, instead of writing "I used a TSA agar plate to isolate my unknown," it is customary to write, "A trypticase soy agar (TSA) plate was used to isolate the unknown." It is also customary to write in the past tense for most of the report. This includes the introduction, the summary, the description of the materials and methods and the results. The present tense is reserved for the conclusions about the results. See the examples given below. Some other general rules that should be followed are: Microbial nomenclature: The name of the bacterium should written and spelled correctly. The name should be italicized or underlined. Italicized is preferred. For example, Staphylococcus aureus. The genus is capitalized but the species is not. After the full genus name is given in the paper, it can be written as S. aureus, but still italicized. This is as long as there in no other...
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...REQUEST FOR BID SPECIFICATIONS LABORATORY/PATHOLOGY SERVICES AND OUTPATIENT SERVICES LAB GENERAL: 1. Must provide total integrated system that includes general laboratory, microbiology, blood bank, pathology, and EMR. 2. Must include instrument or foreign system interfaces currently working in existing client sites for a. Beckman Power Pro Automation Line b. Nova CCX Blood Gas Analyzer c. Beckman Datalink Multiplexor d. Sysmex XT1800 Hematology Analyzer e. Sysmex XE2100 Alpha Hematology Analyzer f. Sysmex SP100 Slide Maker/Stainer g. Beckman CX5 Chemistry Analyzer h. Clinitek Urinalysis Analyzer i. Beckman Advance Coagulation Analyzer j. Urisys Urinalysis Analyzer k. Sysmex UF50 Urine Microscopic Analyzer l. Vitek 2 Microbiology Analyzer m. Bac T Alert Blood Culture Analyzer n. Centaur Immunochemistry Analyzer o. RALS Point of Care System p. Sebia Capillarys 2 Electrophoresis System q. BD ProbeTec PCR System r. BioSite Triage System s. Mayo Reference Lab t. ARUP Reference Lab u. Nichols Institute Reference Lab 3. Must provide database to include the following: CPT codes, LOINC, LMRP for medical necessity. If provided from 3rd party, list sources. 4. Must support multiple laboratories at different locations. Logic must be included that traces all work by location. 5. Must support patient registration through the lab information system. Abbreviated registration required that merges with hospital registration. Only minimal information required...
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...Weekly iLab iLab: Exploring the Microbiology Lab FirstName Tiffani_LastName: Whitehead Section 1 Lab Safety 1. There are four safety equipment items that a lab should have. Identify two of these four items. (2 pts) Biological Safety Cabinet, Eyewash and shower 2. Identify one of the three ways to keep your work area safe. (1 pt) Keep your workspace free of all unnecessary materials 3. There are five recommendations for dressing properly in a lab environment. Name two of these recommendations. (2 pt) Avoid loose fitting items of clothing, Wear appropriate shoes sandals are not allowed. 4. There are several safety tips to protect one’s mucous membranes and broken skin. Identify one of the recommended tips. (1 pt) Do not apply makeup, put in contact lenses, or otherwise perform activities that might introduce lab organisms to your personal space. 5. When working in a microbiology lab, it is important to contain spills and discard materials properly. Describe how a spill should be cleaned up safely in a microbiology lab. (3 pts) 1. Wear a lab coat, disposable gloves, safety glasses or a face shield, and if needed, approved respiratory equipment. 2. Soak a paper towel(s) in an appropriate disinfectant (70% ethanol or fresh 10% bleach solution) and place around the spill area. 3. Working from the outer edges into the center, clean the spill area with fresh towels soaked in the disinfectant. Be sure to decontaminate any areas or surfaces that...
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...Clinical Microbiology Lab Final December 13, 2013 Table of content Gram Stain Technique……………………………………………………………………………………………… page 1 Culture Transfer Technique……………………………………………………………………………………… page 2 Acid-Fast Stain Technique………………………………………………………………………………………… page 3 The importance of the Gram Stain Technique to a physician……………………………………. page 4 The importance of varying shapes/colonies formation of bacteria……………………………. page 5 Spore Stain Technique………………………………………………………………………………………………. page 6 The Importance of incubation/protocol techniques…………………………………………………... page 7 The importance of various types of media for bacterial growth…………………………………. page 7 The importance of biochemical analysis in the microbial process……………………………… page 8 The importance of studying Clinical Microbiology and how the course will assist me in reaching my professional goals……………………….. page 9 Bibliography……………………………………………………………………………………………………………… page 10 Gram Stain Technique The Gram Stain is one of the most important differential stains used in bacteriology. (Cappuccino and Sherman, Microbiology A Laboratory Manual) Using the gram stain it is possible to determine purple gram-positive cells (S. aureus) from pink gram-negative cells (E. coli). The results of the Gram Stain make it possible to identify microorganisms by their shape, number and morphology. In a clinical setting these results can help in treatment by identifying the type of microorganism...
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...Phuong Nguyen Professor H. Valtierra Microbiology-Lab 152 4 December 2012 Unknown Lab Report There are many reasons for doing this unknown exercise report. This unknown lab exercise is applicable to real life situation. The reasons range from medical purposes, such as identifying and characterizing what microorganisms is causing the disease in a patient. Furthermore, treating the patient with the correct medications is another important factor. To identify an unknown bacterium, the lab exercise was done by applying the correct methods in order. The first procedure that was perform for unknown H28 was the quadrant streak on a tryptic soy agar (TSA) plate. The purpose was to check for purity of the bacterium and to identify the colony morphology. Figure 1 shows the following results for unknown H28 colony morphology: irregular colonies, cream color, undulate margin, and flat elevation on a TSA plate. Furthermore, TSA slant streak patterns was perform to identify arborescent growth and tryptic soy broth procedure was perform to identify sediment for growth of unknown H28. The second procedure that was done was a gram stain to determine the gram reaction and Fig. 1. TSA plate shows irregular colonies, cream color, undulate margin, and flat elevation of unknown H28. 1 identify the morphology. The morphology of unknown H28 is bacilli and the arrangement is streptobacilli. After determining that unknown H28 is a gram positive rod, specific biochemical test were performed. The...
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...Introduction Morphological, staining, cultural and biochemical data on the characteristics of different microorganisms was gathered. This gained information can be used to identify bacteria. A scientist by the name of David Bergey was the first person who proposed the system of bacterial classification in which bacteria are grouped according to Gram reaction, metabolism, and morphology. The first edition of Bergey’s Manual of Systematic Bacteriology was published in 1984. This book can be used to identify a microorganism. The purpose of this experiment is to identify an unknown bacteria using the skills learned in microbiology laboratory this semester. Materials and Methods This experiment was conducted at Louisiana State University in Shreveport...
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...Duangjitcharoen, Chaiyavat Chaiyasut, Teruhiko Nitoda, Hiroshi Kanzaki Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages Electronic Journal of Biotechnology, vol. 13, núm. 5, 2010, pp. 1-15, Pontificia Universidad Católica de Valparaíso Chile Available in: http://www.redalyc.org/articulo.oa?id=173318799002 Electronic Journal of Biotechnology, ISSN (Electronic Version): 0717-3458 edbiotec@ucv.cl Pontificia Universidad Católica de Valparaíso Chile How to cite Complete issue More information about this article Journal's homepage www.redalyc.org Non-Profit Academic Project, developed under the Open Acces Initiative Electronic Journal of Biotechnology ISSN: 0717-3458 http://www.ejbiotechnology.info DOI: 10.2225/vol13-issue5-fulltext-1 Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages Duangporn Kantachote1 1 · Pakorn Prachyakij1 · Wilawan Charernjiratrakul1 Metta Ongsakul · Yodsawee Duangjitcharoen2 · Chaiyavat Chaiyasut2 Teruhiko Nitoda3 · Hiroshi Kanzaki3 1 Department of Microbiology, Faculty of Science, Prince of Songkla University, Thailand 2 Department of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University, Thailand 3 The Graduate School of Natural Science and Technology, Okayama University, Japan Corresponding author: duangporn.k@psu.ac...
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...Medications | 1. lisinopril 10mg QD 2. HCTZ 12.5mg QD | Allergies | Height | Weight | Levels of End of Life Care | NKDA | 5”3” | 64kg (141 lbs) | Full Code | Admission Date | Admission Diagnosis | Admitting Physicians | Today | S/P (Status/Post) R total knee replacement | McNasty Ortho surgeon | Scenario Data | Learning Objectives | Note: This simulation is part of a Microbiology course (MICRO 290). The main learning objective is to use a simulation of sepsis to facilitate student visualization and understanding of the mechanisms of key chemical and biochemical events in the human body during a human pathological scenarioBy the end of this scenario, the participant will be able to: 1. Observes and discusses the use of patient history and assessment data in the early identification and management of patients at risk for or with sepsis, septic shock and multi-system organ dysfunction (ANALYSIS). 2. Observes and discusses the possible diagnostic orders and therapies including medications for the management of patients with sepsis, septic shock and multi-system organ dysfunction. Uses factor analysis to calculate doses of medication (COMPREHENSION, APPLICATION). 3. Applies the microbiology concepts learned throughout the course to explain the physiological outcomes involved in sepsis (COMPREHENSION, APPLICATION, ANALYSIS). The microbiology concepts are the following: a. Sepsis and the immune system – Relate the signs (including labs) and symptoms to the...
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...------------------------------------------------- Antimicrobial Susceptibility, MIC and MBC ------------------------------------------------- Microbiology Lab Report 3 Carolina Toscanojara Antibiotic Sensitivity. Objective: Antibiotics are chemical compounds that selectively interfere with the growth of microorganisms while causing little or no damage to the animal or human host. The purpose of this lab was to determine the effectiveness antibiotics against any given bacterium by means of examining the zone of inhibition, the minimum inhibitory concentration, and the minimum bactericidal concentration. Introduction: Chemical antimicrobial agents are chemical compounds that are capable of either preventing the microorganisms for multiplying (bacteriostatic) in order for the immune system of the host to overcome them or killing them completely (bactericidal). Antibiotics taken to improve the symptoms from a disease are called chemotherapeutic drugs; they are produced by a microorganism such as bacterium or fungus and/or are derived from a chemical produced by one. The test carried out to determine which antibiotic will be most successful in treating certain bacteria is called Antibiotic Susceptibility Test (AST) and it is often done by the Kirby-Bauer method, which uses Muller Hinton agar and paper discs containing specific concentrations of antibiotics are placed on the agar surface after rubbed with bacterial specimen. Results of AST are examined for zones of inhibition...
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...Cheese Lab Background: Enzymes are proteins that catalyze or control metabolic reaction. Enzymes work by lowering the amount of activation energy needed so the reaction will happen more quickly. The molecules that an enzyme acts upon are called substrates, the substrate solutions used in this lab were milk and water. In this lab, the enzymes are specific for particular substrates. The enzyme (junket tablet) converts these substrates into different molecules by curdling. If the enzyme concentration required to start the reaction increases and speeds up a reaction, then it will remain unchanged unless certain limiting factors are introduced. Analysis & Discussions: Enzyme concentration: According to figure 5, if the amount of concentration of junket tablet increased, the time for initial curdles to form in room temperature milk decreased. This was evident because the curdles that were formed on the edges of the test tube was much faster as the amount of enzyme concentration increased by 1/5th each. The trend shows the inclination of time versus the concentration of the junket tablet, indicating the presence of a higher amount of enzymes controls the decrease in time. Moreover, the curdles were then formed larger by the last few trials according to table 5. The hypothesis is proven true by figure 5, where the rate of reaction increased as the enzyme concentration increased except at trial four where it decreased slightly. In conclusion, the higher the enzyme concentration...
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...four hours away, which means at least a day’s turnaround. "We have to be very good at repairing instruments ourselves and doing on-site and preventive maintenance," says Nancy Felton, laboratory manager at Marian, a 167bed hospital in Santa Maria, Calif., about equidistant from San Francisco and Los Angeles. Consequently, reliability and user-friendly maintenance and repair are key criteria in the hospital’s purchase of new or replacement instruments. "We want to be able to deal with most problems ourselves," Felton says. Marian Medical Center and other small hospitals (under 200 beds) share similar labmanagement problems: When considering new tests, these labs must justify not only the cost of the equipment, but also the space and training resources they will consume. Turnaround time and volume are also important factors. 1 Practice Management Toolkit © 2011 College of American Pathologists. All rights reserved. At the same time, small hospitals must compete vigorously to attract and retain physicians. And, since these hospitals are often the sole providers in their areas, they emphasize high-quality, wide-ranging patient care. "Being small is no excuse for being less than excellent," says Edward Ewing, DO, laboratory medical director for Waynesboro Hospital, a 62-bed facility in central Pennsylvania. Testing, Testing In deciding...
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...BIOL334 Microbiology & Immunology Textbook Content I. Lecture 1: Syllabus II. Reading Assignments for Chapter 1 A. Lecture 2: 1. Define Microbiology & Microorganisms 2. sec 1-1: pp. 1-4: Members of the microbial world B. Lecture 3: 3. Why is microbiology important? 4. Integrated content of Sec 1.4, pp. 17-19 C. Self-Learning: History of Microbiology 5. Sec1.3, pp. 11-16 please read! III. Chapter 2: All sections will be covered from this chapter but keep in mind, BIOL201 emphasizes ALL microscopes; I will focus on the bright-field light microscope, discuss staining, and then provide a brief overview of other types of microscopes. D. Order of content in lecture: 2.1, 2.2 Light Microscopes emphasizing bright-field microscopy up to p. 26, Staining: 2.3 p. 31-34; Sec 2.2; Other microscopes in sec 2-2 p. 26-31; sec 2.4-2.5. E. This will be covered 8/26, 8/28, and part of 9/2. F. Have a general idea on how all of the microscopes work that I do not cover in depth. My emphasis will be on: Brightfield, fluorescence, and electron microscopy. Cell Structure- Ideal Lecture order: (Chapter 3 &4) DATE | Text Chapter | Lecture Topic | | | Prokaryotic Cell Structure & Function. Ch 3 discusses the structure of Bacteria while Ch 4 discusses Archaea. Note: Ch. 5- Eukaryotic Cell Structure was covered in Biol-201. I will not cover this information but I will...
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...Antibiotic Resistance and Gram Staining of Unknown Bacterial Samples Microbiology Lab July 1, 2013 Abstract: In this final laboratory assignment, each student was given an agar plate. Each plate was swiped with a pure culture bacteria sample. Where the sample came from and what kind of bacteria was unknown to the students. We were asked to observe the colony morphology on our plates and to perform the antibiotic sensitivity/resistance test. The plates were put into a 37° incubator oven. The next day the plates were taken out of the oven and we calculated the zone of inhibition. We then performed a gram stain of our bacteria and analyzed it under a microscope. We were then able to assess what kind of bacteria we had and if it was gram positive or negative. Introduction: Simple staining (the use of a single stain) allows a microbiologist to observe the morphology (shape) and arrangement of bacteria. In order to classify bacteria into different groups a differential staining procedure must be done. A differential stain involves the use of two or more stains. Depending on the components of the bacterial cell wall or outer layers, the bacteria will either retain the primary stain or have the primary stain removed in a decolorizing step and then retain the secondary stain. The gram stain is the most common differential stain used in the microbiology laboratory to categorize bacteria. The primary stain is the cationic dye Crystal violet and the secondary...
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