...Experiment 8. The hydrolysis of starch with hydrochloric acid (a) Prepare a water bath by half filling a 250 cm3 beaker with warm water and heating it to boiling point on a tripod and gauze, with a Bunsen burner. When the water boils, reduce the flame to keep the water at boiling point. (b) Label four test-tubes 1-4. (c) Copy the table given below into your notebook. (d) In each tube place 5 cm3 3% starch solution. (e) Using a syringe or graduated pipette, add 3 cm3 Benedict's solution to the starch solution in tube 1 and place the tube in the boiling water bath for five minutes. (f) Rinse the syringe or pipette and use it to add 1 cm3 dilute hydrochloric acid to the starch solution in each of tubes 2, 3 and 4. Note the time and place all three tubes in the water bath. (They will be removed at five, ten and fifteen minutes respectively). (g) Remember to remove tube 1 from the water bath after five minutes if you have not already done so. (h) After five minutes, remove tube 2 from the water bath and cool it under the tap. Neutralize the acid by adding solid sodium bicarbonate, a little at a time, until the addition of one portion produces no fizzing. Place tube in the rack and return to tube 3. (i) After ten minutes in the water bath, remove tube 3, cool and neutralize the contents as described in (h). Place the tube in the rack. (j) After fifteen minutes in the water bath, remove tube 4; cool and neutralize as before, and place it in the rack...
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...Title: Identification of Biochemical in Their Pure Form Objective: To identify the components of the solution in its pure form with various food tests and state the justifications. Observations: Tests | Observations | Reducing sugar test a) Glucose | * White glucose solution changes colour to blue when Benedict’s solution is added. After the solution is heated, the blue solution forms moderate amount of brick red precipitate. | b) Fructose | * White fructose solution changes colour to blue when Benedict’s solution is added. After heating, blue solution forms more amount of brick red precipitate. | c) Lactose | * White lactose solution changes colour to blue when Benedict’s solution is added. After heating, blue solution forms least amount of brick red precipitate. Solution still have a slight blue colour. | Non-reducing sugar test a) Sucrose | * Sucrose solution is neutralized with hydrochloric acid and sodium hydroxide solution. No changes is observed. Blue colour forms when the solution is added with Benedict’s solution. After heating, brick red precipitate is formed. | Iodine test a) Starch | * Blue-black precipitate is formed after 3 drops of iodine is added. | | Sudan III test | * Oil and distilled water were immiscible forming two separate layer. When Sudan III is added, fats globules are stained red. Fat layers forms at the top layer. The solution was opaque and forms at the bottom layer. | Emulsion test |...
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... Introduction Carbohydrates, proteins and lipids are all organic macromolecules composed of polymers which are made up of smaller simpler subunits (monomers). These macromolecules have unique characteristics that allow them to carry out complex functions. In this lab, four (4) tests will be conducted: the Benedict test (for reducing sugar) BNT test; Iodine test (for starch) BIU test; Biuret test (for protein) IDN test; and Sudan III test (for lipids) SDN test. The first test conducted would be the Benedict test. Carbohydrates include sugars, glycogen, starches and cellulose. Three substances will be tested for reducing sugars. These substances include sucrose, glucose and distilled water (H2O). If any of the substances are negative for non-reducing sugars it will have a blue color. If it positive for reducing sugar it will have a yellow to green to reddish orange color for weak to strong presence of the sugar respectively. The Idoine test for starch would be the second test preformed. Staining by distinguishes starch from monosaccharides, diaaccharides and other polysaccharides. Two substances potatoes and distilled water will be tested for starch. If starch is present in any of the substances the iodine will turn it bluish-black. If the...
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...Class : FHSB1214 P16 Group Member : Lim Phei Ching Lee Jer Win Chong Hui Li Title : Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions . Objective : To Investigate of Effect of Salive and Hydrochloric Acid in Carbohydrate Solutions . Apparatus & Equipment : Boiling tubes , Beaker , Graduated plastic dropper , Water bath, ~ 37 oC , Water bath, ~ 95oC Materials : Carbohydrate solution A , Carbohydrate solution B , Benedict‟s solution , 3 M Hydrochloric acid , 3 M Sodium hydroxide , Iodine solution Procedures : Part 1 1. Two boiling tubes containing 1 ml solution A and 1 ml of solution B were prepared respectively . 1ml of Benedict solution was added into each tube . Both tubes were heated together in the 95°C water bath for two minutes . Results were recorded in table 1 . 2. A few drops of solution A and B were added separately on a white tile . 1-2 drops of iodine solution were added on each solution . Part 2 3. Boiling tubes were labeled 1,2,3,4 . 2 ml of solution B was pipette into each of four boiling tubes . 4. Tubes 1 and 2 were placed in a water bath of 37°C to heat up the solution . 5. Small beaker was salivated till it reached about 5 ml . 6. This step was done approximately at the same time . 2ml of saliva prepared was pipetted into tubes 1 and 4 . The contents of tubes were shaken well to ensure through mixing . 2ml of HCL...
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...the presence of these important compounds in food by using chemical reagents that react in predictable ways in the presence of these nutrients. Please refer to the notes given above on: How to record qualitative data. (Marks will be awarded based on proper recording.) What to do if you don’t obtain the desired results. Flowchart Students will be allowed to proceed with the experiment only if they have come into the laboratory with a flowchart of the day’s experiment. Lab manual version 6_201505 FHSB1214 Biology I & FHSC1214 Fundamentals of Cell Biology 14 Procedures: Part 1: Identification of Carbohydrates (A) Test for reducing sugars The reducing sugars include all monosaccharide, such as glucose and fructose, and some disaccharides, such as maltose and lactose, using 0.1 – 1% sugar solutions. Common tests for reducing sugars include Benedict’s test (described below) and Fehling’s test (not described here). See ‘basis of test’ below for explanation of the following reaction: Benedict’s test for...
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...Experiment 8. The hydrolysis of starch with hydrochloric acid (a) Prepare a water bath by half filling a 250 cm3 beaker with warm water and heating it to boiling point on a tripod and gauze, with a Bunsen burner. When the water boils, reduce the flame to keep the water at boiling point. (b) Label four test-tubes 1-4. (c) Copy the table given below into your notebook. (d) In each tube place 5 cm3 3% starch solution. (e) Using a syringe or graduated pipette, add 3 cm3 Benedict's solution to the starch solution in tube 1 and place the tube in the boiling water bath for five minutes. (f) Rinse the syringe or pipette and use it to add 1 cm3 dilute hydrochloric acid to the starch solution in each of tubes 2, 3 and 4. Note the time and place all three tubes in the water bath. (They will be removed at five, ten and fifteen minutes respectively). (g) Remember to remove tube 1 from the water bath after five minutes if you have not already done so. (h) After five minutes, remove tube 2 from the water bath and cool it under the tap. Neutralize the acid by adding solid sodium bicarbonate, a little at a time, until the addition of one portion produces no fizzing. Place tube in the rack and return to tube 3. (i) After ten minutes in the water bath, remove tube 3, cool and neutralize the contents as described in (h). Place the tube in the rack. (j) After fifteen minutes in the water bath, remove tube 4; cool and neutralize as before, and place it in the rack...
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...Objective: To identify the components of the solution in its pure form with various food tests and state the justifications. Observations: Tests | Observations | Reducing sugar test a) Glucose | * White glucose solution changes colour to blue when Benedict’s solution is added. After the solution is heated, the blue solution forms moderate amount of brick red precipitate. | b) Fructose | * White fructose solution changes colour to blue when Benedict’s solution is added. After heating, blue solution forms more amount of brick red precipitate. | c) Lactose | * White lactose solution changes colour to blue when Benedict’s solution is added. After heating, blue solution forms least amount of brick red precipitate. Solution still have a slight blue colour. | Non-reducing sugar test a) Sucrose | * Sucrose solution is neutralized with hydrochloric acid and sodium hydroxide solution. No changes is observed. Blue colour forms when the solution is added with Benedict’s solution. After heating, brick red precipitate is formed. | Iodine test a) Starch | * Blue-black precipitate is formed after 3 drops of iodine is added. | | Sudan III test | * Oil and distilled water were immiscible forming two separate layer. When Sudan III is added, fats globules are stained red. Fat layers forms at the top layer. The solution was opaque and forms at the bottom layer. | Emulsion test | * After shaking- White, opaque solution was formed. * After...
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...Class : FHSB1214 P16 Group Member : Lim Phei Ching Lee Jer Win Chong Hui Li Title : Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions . Objective : To Investigate of Effect of Salive and Hydrochloric Acid in Carbohydrate Solutions . Apparatus & Equipment : Boiling tubes , Beaker , Graduated plastic dropper , Water bath, ~ 37 oC , Water bath, ~ 95oC Materials : Carbohydrate solution A , Carbohydrate solution B , Benedict‟s solution , 3 M Hydrochloric acid , 3 M Sodium hydroxide , Iodine solution Procedures : Part 1 1. Two boiling tubes containing 1 ml solution A and 1 ml of solution B were prepared respectively . 1ml of Benedict solution was added into each tube . Both tubes were heated together in the 95°C water bath for two minutes . Results were recorded in table 1 . 2. A few drops of solution A and B were added separately on a white tile . 1-2 drops of iodine solution were added on each solution . Part 2 3. Boiling tubes were labeled 1,2,3,4 . 2 ml of solution B was pipette into each of four boiling tubes . 4. Tubes 1 and 2 were placed in a water bath of 37°C to heat up the solution . 5. Small beaker was salivated till it reached about 5 ml . 6. This step was done approximately at the same time . 2ml of saliva prepared was pipetted into tubes 1 and 4 . The contents of tubes were shaken well to ensure through mixing . 2ml of...
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...internalised in the price of the good, this may reduce demand for the good and the level of its production, eating sugary food and drink will increase the likelihood of obesity, early death, depression and certain heart disease. Poor health will also adversely affects work and productivity. Higher prices would discourage people from consuming unhealthy foods. Reducing consumption of sugary foods would have a significant impact in improving health. Also, adding tax on sugar can create an incentive for firms to supply alternatives which are healthier. If firms have incentives to promote healthier foods and drinks with substantially lower sugar content, then consumers will to an extent follow the supply. Moreover, taxes raise revenue for government, this can be spent on alternatives like tax on cigarettes could be used for funding government services to help people to stop smoking. However, it is difficult to measure the level of negative externality. Demand for sugary and drink tend to be price inelastic, so higher taxes will not reduce demand much. Sugary foods are demerit goods. Demerit goods are goods whose consumption is regarded as being harmful to the people that consume them, but people are usually unaware about the harm that the demerit goods can cause. Due to imperfect information, consumers might not have enough information on how it might affect their health, so their demand for the sugary food is higher than it should be and it...
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...food. One of the main drivers has been the consumption of food in rapidly developing nations China and India. Rising consumption in meat leads to more demand in corn to feed animals. However farmers have been producing corn and soy beans for other reasons, mainly to create bio-fuels out of them. And to make matters even worse, governments are seeing bio-fuel as a solution for the increasing global warming situation. They are making a solution for global warming and creating another problem food is getting more expensive. The reason behind this is because farmers get subsidies from the government thus leaving the production of their crops as food behind. This makes it more expensive for other farmers to feed animals that will later lead to food in the market. These are 2 big problems that are happening right now because we want to protect the environment but we all know that we do not want to go hungry doing it. What is unfortunate in this situation is that high tariffs are shutting out producers of alternative products that can be turned into bio-fuels, most notable sugar cane, from the U.S. and EU. One of the most notably sugar cane producers (Brazil) confronts import tariffs of about 25% in the U.S. and 50% in the EU. These import tariffs raise the price of sugar and makes it uncompetitive with corn and soy beans thus breaking the chance of converting sugar into bio-fuels and ethanol. It is an unfortunate situation because sugar is seen as a more environmental friendly raw...
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...wrong with soda is that it is filled with carbs, calories, and most importantly sugar. Soda has caused many people to go obese and can cause some serious health issues including diabetes and Alzheimer's disease. Soda is very bad for everyone because of the high amounts of sugar, carbs, and sodium which is causing obesity. A way to prevent the consumption of soda is to put a tax on the bubbly beverage itself. If the cost went up 10% then not many people would buy it because of the price increase. “Currently, 33 states have sales taxes on soft drinks (mean tax rate, 5.2%)” (A Soda Tax Would Help Reduce Obesity). Yes, there is a tax on soft drinks in a lot of states in the U.S. but it’s not enough for people to stop purchasing these soft drinks. A way to prevent people to stop the purchase of these products is to increase the tax from 5.2% to at least 10% because they would feel that it would be too expensive so they would get a water or lemonade instead....
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...major mechanical problems requiring massive repairs. The human body has certain limitations and guidelines that must be very closely adhered. The human body must maintain critical balances to remain healthy and working the way it was intended and to be able to balance the effects of food on human beings. Nutrition is a basic biological influence on human behavior. Consuming large amounts of “junk food,” like sweetened boxed cereals, candy, carbonated drinks, potato chips, and fast food cause depression, hyperactivity, and even anger in children and teenagers. The amount of candy eaten by children and sometimes adults also causes major sugar imbalances to occur in the human body. In the article “Food Traditions: The Thread that Links Generations,” Bonny Wolf explains why “Food imprinting is strong’’ (135). There is a daily absorption of high-sugar or artificially sweetened carbonated drinks using such poisonous artificial ingredients as NutraSweet, Aspartame, Sucralose, inordinate amounts of high-fructose corn syrup, and many other physically dangerous additives and...
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...Everglades with its unique ‘sheet flow of water’, had made the place one of the hottest tourist spots. The scenario started changing in the late 1980’s with the beginning of developments like creation of agricultural farms for growing sugarcane and vegetables, building up of new canals and levees for protecting the farms and the cities from floods and increase in the infrastructure in the east of the everglades. Some parts of the marshland started facing drought and others flood. Earlier it used to cover an area of 10,360 kms but today it has been reduced to half its size. The primary reason for this condition was the pollution caused by companies like Big sugar, construction companies and various other factors (Marjory Stoneman Doughlas, 1969). The Big sugars comprise of a group of sugarcane growers mainly U.S sugars and are primarily responsible for destroying the Everglades. Farmers of these agricultural farms use high content phosphorus fertilizers in excessive quantities, to improve the quality of their soil. The runoff water from these sugarcane farms carrying the killer phosphorus gets drained into the Everglades through canal-water discharge from Lake Okeechobee and the Everglades agricultural area. It was revealed that this overdose of nutrient results in the overgrowth of unwanted plant and algae species or eutrophication,...
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...increase and hopefully result in people buying less of them. I agree, the United States government should implement a junk food tax. Some people could argue that this tax wouldn't make a difference as far as reducing the amount of junk food sold. But there is evidence that shows it could be successful. First off, there...
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...How To Avoid Junk Food Consumption By Jared Lee Ads by Google Healthy Eating www.xo.gr Ποτέ δεν ήταν τόσο εύκολο να βρεις αυτό που ψάχνεις: Χρυσός Οδηγός! Leptin Slimming Green Tea www.green-coffee-800.com Weight Management Green Tea Offers for retail and wholesale Νέο Κινητό Nokia www.nokia.com Όλα τα στοιχεία για το προηγμένο Nokia Smartphone. Ενημερώσου εδώ! Companies like McDonald, Pizza Hut and Dominos earn million of dollars by selling food items that are widely considered in the medical fraternity as "junk". Consumption of junk food is an addiction that is affecting millions of people across the globe including children, teenagers and even adults. These foods are rich in harmful ingredients like fat, sugar, salt and preservatives that can result in serious medical complications such as coronary heart diseases, obesity, hypertension, diabetes and even cancer. Hence, it is very important to avoid these foods in the diet. Below listed are some useful ways of avoiding junk food consumption. 1. One should consume at least 8 big glasses of water everyday. This ensures effective removal of any toxins present inside the tissues and blood vessels. 2. Hunger is the biggest factor that makes a person vulnerable to junk food consumption. It is important to feed the stomach periodically with foods like nuts, cereals, fruits and milk. 3. It is important to change the diet pattern for a healthy living. One should start with a heavy breakfast, a reduced lunch and a very meager...
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