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Antiseptics Lab Report

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Words 682
Pages 3
Yasmin Martinez
Biology 2310
Lab Report I
Antibiotics, Antiseptics, and Disinfectants
June 22, 2018

Introduction As the world continues to evolve, bacteria evolves along with it. This can begin to impact the effect of antimicrobials, which are used to inhibit or kill the growth of microorganisms. Antimicrobial testing is important to determine the susceptibility within a given agent. It is also important to consider resistance of an antimicrobial agent to a given microorganism. Antimicrobial resistance occurs when a given drug loses its effect due to the microbes changing, either because of mutation or they acquire new genetic information from other microbes.1

Materials and Methods The materials required for this lab are the four bacterial broth cultures: Staphylococcus saprophyticus, Escherichia coli, Pseudomonas fluorescens, and Bacillus subtilis. Eight trypticase soy agar (TSA) plates, a Kirby-Bauer Antibiotic Disk Dispenser, a container with sterile filter paper disk, another container with 70% alcohol, forceps, sterile swabs, Hydrogen Peroxide, Bleach, Antiseptic Mouth Wash, …show more content…
With the measured zones of inhibition, E. coli was susceptible to K30, Am10, S10, C30, and resistant to E15 and P10. P. fluorescens was susceptible to
Antibiotic Code Concentration standards Resistant Intermediate Susceptible
Kanamycin K30 30 mcg (-- removed HTML --) =18
Ampicillin Am10 10 mcg a <=27 b (-- removed HTML --) =28
>=17
Erythromycin E15 15 mcg (-- removed HTML --) =23
Penicillin P10 10 units a NS b <=28 NS NS
>=29
Streptomycin S10 10 mcg a <=11 b NS 12-14
NS >=15
NS
Chloramphenicol C30 30 mcg (-- removed HTML --) =18

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