...Cellular Structures and Pathogenicity Brittany McMahon ITT Technical College Cellular structures have different impacts on the ability for bacteria to cause disease. Mainly the cells external structures have the biggest effect to adhere to the cells to cause disease. One structure being the pili, a rigid, thin fiber made of protein that expands from the cell surface. The main function are to adhere specific bacterial cells to specific surfaces. There are typically only one or two pili per cell (Hartsock, Angela 2016). Pili that is coated with adhesins can determine the mucosal surface of the respiratory tract and will only adhere to that specific surface to infect those cells (Hartsock, Angela 2016). This bacteria is known as Bordetella. The fimbria is another external structure that is similar to the pili. They are short, filamentous structures that are in large numbers that help in attachment to surfaces (Hartsock, Angela 2016). Like pili the fimbria target tissues they believe will be the best host. Flagella has a completely different function from the pili and fimbria. The main function for flagellum is motility. They are long, tail like appendages attached to bacterial cells that allow the movement (Schuhmacher, J., Rossmann, F., Dempwolff, F., Knauer, C., Altegoer, F., Steinchen, W., Bange, G. 2015). Flagella is made of protein called flagellin. The proteins form a long chain that look like a helical shape. From the cell membrane the flagellum is wide and attaches...
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...1.1 Compare the ultra structure of a prokaryotic and a eukaryotic cell. ANSWER: Prokaryotic and Eukaryotic organisms: are composed of cells, the basic unit of life, with each cell surrounded by a cell membrane while membranes are composed of phospholipids, proteins and carbohydrates arranged in a fluid mosaic structure. The main different between the both cell organism is nucleus: Prokaryotic cells are smaller and simpler organisms without no nucleus but most are unicellular, while eukaryotic cells are larger organisms with a nucleus but often multicellular. Differences Prokaryotic cells Eukaryotic cells Protein DNA is circular, without protein. DNA is associated with protein to form chromatin. Cell wall Cell division is by binary fusion Usually...
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...MARCETTE Biology Survey 09/15/2014 Cell Structure report – Mitochondria All living organisms on Earth are divided into cells. The main concept of cell theory is that cells are the basic structural unit for all organisms. Cells are small compartments that hold the biological equipment necessary to keep an organism alive and successful. So, I would think that cell structures would be all the parts that makes a cell work and maintain the living organism alive. Mitochondria are known as the powerhouses of the cell. They are organelles that act like a digestive system that takes in nutrients, breaks them down, and creates usable energy for the cell. Mitochondria are a membrane bound cellular structure and is found in most of the eukaryotic cells. The way that the mitochondria works is the matrix is filled with water and enzymes. Those proteins take food molecules and combine them with oxygen. The mitochondria are the only place in the cell where oxygen can be combined with the food molecules. After the oxygen is added, the material can be digested. They are working organelles that maintain the cell with full energy. The most important function of the mitochondria is to produce energy. The simpler molecules of nutrition are sent to the mitochondria to be processed and to produce charged molecules. These charged molecules combine with oxygen and produce ATP molecules. This process is known as oxidative phosphorylation. Mitochondria help the cells to maintain proper concentration of...
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...Assignment: SCIE207 Phase 2 Lab Report Title: Animal and Plant Cell Structures 1. Animal Cell: [pic] |Number |Cell Structure |Description and Function | |1 |Nuclear Pore |Nuclear pores are large protein structures that cross| | | |the nuclear envelope, which is the double membrane | | | |inclosing the eukaryotic cell nucleus. The function | | | |of a nuclear pore is to control the way of molecules | | | |between the nucleus and cytoplasm, allowing some | | | |material to go through the membrane. | |2 |Chromatin (DNA) |Chromatin is the combined material of DNA and | | | |proteins. Chromatins are what make up the entire | | | |nucleus of a cell. The function of a chromatin is to | | |...
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...and Plant Cell Structures Instructions: Your lab report will consist of the completed tables. Label each structure of the plant and animal cell with its description and function in the provided tables. When your lab report is complete, submit this document to your instructor in your assignment box. 1. Animal cell: Observe the diagram that shows the components of an animal cell. Using the textbook and virtual library resources, fill in the following table: Animal Cell Number Cell Structure Description and Function 1 Chromatin (DNA) The primary functions are to package DNA into a smaller volume to fit the cell, to enforce DNA macromolecule to allow mitosis, to prevent DNA damage, and to control gene expression and DNA replications 2 Nucleolus The largest structure in the nucleus of eukaryotic cells where it primarily serves as the site of ribosome synthesis and assembly. 3 Nucleus The nucleus is basically the command center of the cell, it controls eating, movement, and reproduction. 4 Nuclear Pore Are large protein complexes that serve as the pathways for exchange of the materials between the nucleus and cytoplasm 5 Nuclear Envelope A highly regulated membrane barrier that separates the nucleus from the cytoplasm in eukaryotic cells. 6 Centrioles Self-replicating organelles made up of nine bundles of microtubules and appear to help in organizing cell division. 7 Cilia and Flagella Hair like organelles that branch out from the surface of the cell and are essential...
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...from rapid muscular contraction. It is similar to explosive strength and involves a large proportion of fast twitch muscle fibres and the ability to recruit as many motor units as quickly as possible. Power is produced through the skeletal muscles. Although skeletal muscle cells come in different shapes and sizes the main structure of a skeletal muscle cell remains the same. The Epimysium protects the muscle from friction against other muscles and bones. It also continues at the end of the muscle to form (along with...
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...Cellular Structures and Pathogenicity All the various surface components of a bacterial cell are important in the ecology because they mediate the contact of the bacterium possesses result from immediate contact with its environment. Its must use its surface components to assess the environment and respond in a way that supports its own existence and survival in that environment. The surface properties of a membrane and cell envelope, including capsules, glycocalyx, slayers, peptidoglycan and lps, and the other surface structures, such as flagella and pili or fimbriae. Bacterial surface components may have a primary biological function that has nothing to do with path ogenicity. However, there are endless examples wherein a bacterial surface component plays an indispensable role in the pathogenesis of infectious disease. Bacterial structures may act as permeability barriers that allow selective passage of nutrients and exclusion of harmful substances; adhesions used to attach or adhere to specific surfaces or tissues; enzymes to mediate specific reactions on the cell surface important in the survival of the organism; protective structures against phagocytic engulfment or killing; antigenic disguises to bypass activation oh host immune defenses; endotoxins, generally cell wall components, that cause an inflammatory response in the host; “sensing proteins” that can respond to temperature, osmolality, salinity, light, oxygen, genome of the cell that will cause expression of some...
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...Colloids and Surfaces B: Biointerfaces 102 (2013) 412–419 Contents lists available at SciVerse ScienceDirect Colloids and Surfaces B: Biointerfaces journal homepage: www.elsevier.com/locate/colsurfb Non-cytotoxic antibacterial silver–coumarin complex doped sol–gel coatings Swarna Jaiswal a,b , Kunal Bhattacharya c , Maeve Sullivan d , Maureen Walsh d , Bernadette S. Creaven d , Fathima Laffir e , Brendan Duffy a,∗ , Patrick McHale b a Centre for Research in Engineering Surface Technology (CREST), FOCAS Institute, Dublin Institute of Technology, Dublin 8, Ireland School of Biological Sciences, Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland c Nanolab Research Centre, FOCAS Institute, Dublin Institute of Technology, Dublin 8, Ireland d Centre for Pharmaceutical R&D, School of Science, Institute of Technology, Tallaght, Dublin 24, Ireland e Materials & Surface Science Institute, University of Limerick, Dublin, Ireland b a r t i c l e i n f o a b s t r a c t Microbial colonisation on clinical and industrial surfaces is currently of global concern and silane based sol–gel coatings are being proposed as potential solutions. Sol–gels are chemically inert, stable and homogeneous and can be designed to act as a reservoir for releasing antimicrobial agents over extended time periods. In the present study, silver nitrate (AgN) and a series of silver coumarin complexes based on coumarin-3-carboxylatosilver (AgC) and it is 6, 7 and 8 hydroxylated analogues...
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...this book do not intend disrespect for any particular patient population or racial or ethnic group but are solely presented as memory devices to assist in the learning of a complex and important medical subject. We welcome suggestions for future editions. 1) Write in a conversational style for rapid assimilation. 2) Include numerous figures serving as "visual memory tools" and summary charts at the end of each chapter. These can be used for "cram sessions" after the concepts have been studied in the text. 3) Concentrate more on clinical and infectious disease issues that are both interesting and vital to the actual practice of medicine. MARK GLADWIN, MD BILL TRATTLER, MD D CONTENTS Preface v PART 1 1 2 3 BACTERIAL TAXONOMY CELL STRUCTURES, VIRULENCE FACTORS, and TOXINS...
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...analyzed it under a microscope. We were then able to assess what kind of bacteria we had and if it was gram positive or negative. Introduction: Simple staining (the use of a single stain) allows a microbiologist to observe the morphology (shape) and arrangement of bacteria. In order to classify bacteria into different groups a differential staining procedure must be done. A differential stain involves the use of two or more stains. Depending on the components of the bacterial cell wall or outer layers, the bacteria will either retain the primary stain or have the primary stain removed in a decolorizing step and then retain the secondary stain. The gram stain is the most common differential stain used in the microbiology laboratory to categorize bacteria. The primary stain is the cationic dye Crystal violet and the secondary stain is the cationic dye Safranin. Since both stains are cationic, they are both attracted to the negatively charged particles in the bacterial cell wall. So what causes some bacteria to retain the Crystal violet stain while others are decolorized by the alcohol and then pick up the Safranin stain? The current theory behind gram staining is that the crystal violet enters the...
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...Difference between a cell wall And a Gram Stain Janet Myers 12:30p.m. Tuesday February 23 2016 Introduction: Gram staining is based on the ability of bacteria cell wall to retaining the crystal violet dye during solvent treatment. The cell walls for Gram-positive microorganisms have a higher peptidoglycan and lower lipid content than gram-negative bacteria. Bacteria cell walls are stained by the crystal violet. Iodine is subsequently added as a mordant to form the crystal violet-iodine complex so that the dye cannot be removed easily. This step is commonly referred to as fixing the dye. However, subsequent treatment with a decolorizer, which is a mixed solvent of ethanol and acetone, dissolves the lipid layer from the gram-negative cells. The removal of the lipid...
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...Chromatography INTRODUCTION IN THIS LAB, WE WILL BE EXPRESSING THE GREEN FLUORESCENT PROTEIN (GFP) IN BACTERIA AND PURIFYING IT USING COLUMN CHROMATOGRAPHY. THE SPECIFIC TYPE OF CHROMATOGRAPHY WE WILL BE USING IS HYDROPHOBIC INTERACTION CHROMATOGRAPHY (HIC). THE FOLLOWING IS INFORMATION FROM BIO-RAD INC., THE SUPPLIER OF THE REAGENTS: GFP has several stretches of hydrophobic amino acids, which results in the total protein being very hydrophobic. When the supernatant, rich in GFP, is passed over a HIC column in a highly salty buffer (Binding Buffer), the hydrophobic regions of the GFP stick to the HIC beads. Other proteins which are less hydrophobic (or more hydrophilic) pass right through the column. This single procedure allows the purification of GFP from a complex mixture of bacterial proteins. Loading the GFP supernatant onto the chromatography column When students load the GFP supernatant onto their columns, it is very important that they do not disturb the upper surface of the column bed when performing the chromatography procedure. The column matrix should have a relatively flat upper surface. A slightly uneven column bed will not drastically affect the procedure. However, subsequent steps of loading, washing, and eluting should minimize disrupting the column such that beads "fluff up" into the buffer. When loading the GFP supernatant onto the column, the pipette tip should be inserted into the column and should rest against the side of the column...
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...Microbiology lab study guide Exam 1 Know step by step instructions on use of microscope to view a specimen (image remains in focus even when changing objective lens – parfocal) * Bright field- Dark image against bright background. * Dark field- Field surrounding the specimen appears black while object is brightly illuminated. * Phase contrast- Specimens appear as various levels of “darks” against a bright background. * Fluorescence- fluorescence dye is used which emits fluorescence under UV light.. * Total Magnification = Magnification by the objective lens X Magnification by ocular lens. * FOCUS MICROSCOPE PROPERLY by starting with scanning lens and moving up each objective, using scope’s parfocal property Know step by step instructions for ocular micrometer calibration using the stage micrometer * Calibration = Stage Micrometer /Ocular Micrometer * Stage Micrometer: mm convert to um * Ocular Micrometer: 0 – 100 Ocular Units * EX: 0.2mm X 1,000 um = 200 um * 1mm * Placed stage micrometer superimposed by the ocular micrometer. * First left line of the ocular micrometer aligned with one of the marks on the stage micrometer * EX 25 of ocular micrometer on 8 major lines of the stage micrometer are perfectly aligned= 800 µm ÷ 25 ocular units = 32 um/OU * Record more than one measurement * Calibrate each magnification * Know the different parts of the microscope...
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...To discover my unknown, I first conducted the gram test on my species. The result was gram positive coccus. After that I did the catalase test which determines if the bacteria can breakdown hydrogen peroxide into oxygen and water. My unknown was catalase positive which lead to three genera, one of which my unknown could be. The three genera were Micrococcus, Planococcus, Staphylococcus. By doing the SIM motility test I learned that my unknown was nonmotile. With the FTM tube test I learned that my unknown was a facultative anaerobe. The sugar fermentation tubes resulted in my species fermenting acid but were negative for gas. These tests helped me decide that my unknown’s genius was Staphylococcus. Using the white key, I got the best fit match for Staphylococcus epidermidis. Of the three Staphylococcus options in the white key, Staphylococcus aureus had a gold pigment which my unknown was negative for. Staphylococcus saprophyticus was negative for nitrate reduction, and an acid former for mannitol unlike my unknown. Therefore, Staphylococcus epidermidis had the most matches on the white key. There were no tests which I had to redo due to inconclusiveness. For the litmus milk reaction, my organism was acid, alkaline and reduction negative, coagulation and peptonization positive. On the white key my unknown did not match any of the option listed for the genus Staphylococcus. Compared to Bergey’s Manuel of Systematic Bacteriology, my organism matched the many descriptions listed...
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...“Choose your unknown wisely” were the words that our instructor used when we were picking our unknown bacterial microorganism. Each person was paired with another member of the class, and was expected to work as a team to figure out the unknown that was chosen. Using steps and experiments from the Bergey’s Manual, we were suppose to figure out the unknown and how the bacteria we had chosen was classified and characterized. My hypothesis when first starting the test was that this unknown was going to be something we had already dealt with in class. I thought our unknown would be Escherichia coli. Materials and Methods When we chose our unknown bacterial microorganism, it had the number one on the side. The broth had a one in turbidity on a scale from one (being the lowest) to four (being the highest). To see more clearly in the tube, swirling was used for sediment to be seen. The first experiment we did was testing the unknown to see if it was a gram-negative bacterium or whether it was a gram-positive bacterium. Majority of the bacteria that had been handled with in class fell under gram negative. How to tell whether the bacteria is gram negative or gram positive is by staining the bacteria and watching it change it’s color. If the bacteria turn purple, that would show that the bacteria are gram-positive. If the bacteria turn red, then that would explain that it is gram-negative. Another test we did on the bacteria was a streak plate method, which would show how many colonies...
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