...Microbiology is requires not only an academic understanding of the microscopic world but also a practical understanding of lab techniques and procedures used to identify, control, and manipulate microorganisms. The proper identification of a microorganism is not only important in a microbiology lab but also in the medical, industrial, and pharmaceutical fields. In this lab report, lab techniques and procedures learned during this course were performed to assess each students’ practical knowledge in microbiology. 6In area of fields I mention earlier microbiology is very important to our vaccination and antibiotics we are using, understand that microorganism play a key role in maintaining life on earth, fixing gases and breaking down dead plant and animal matter into simpler substances that are used at the beginning of the food chain 6. Biotechnologists can also exploit the activities of microbes to benefit humans, such as in the production of medicines, enzymes and food. The goal of this lab report is 1) to demonstrate comprehension of the methods and lab techniques learned during the semester 2) to explain the tests performed on each isolated unknown that led to the identification of each unknown 3) and to give a background on the characteristics, pathogenicity and some uses of one of the identified unknowns. II. Introduction In this lab report I will discuss how I came to find my two unknown bacteria. Each bacteria have undergo many different test to eventually identifying...
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...MBK – Lab Report Name: __Jade Smart___ Section: ___________________ Aseptic Technique and Culturing Microbes Part 3: Generating Microbial Cultures: Observe your organisms after 24 hours to assess the growth patterns of all tubes. If there is no observable growth allow the tubes to incubate an additional 24 hours. Record your observations. Questions: A. What is the difference between a bactericidal and bacteriostatic agent? Between sterilization and disinfecting? The difference between the two is that bactericidal kills bacteria directly. While bacteriostatic stops the bacteria from growing. Bactericidal will injure the plasma membrane and the cell will leak out, killing it. Bacteriostatic stops bacteria from replicating. The main difference between sterilization and disinfection is, that sterilization kills all microorganisms, while disinfection eliminates harmful microorganisms from inanimate objects and surfaces. Sources: http://study.com/academy/lesson/types-of-antibiotics-bacteriocidal-vsbacteriostatic-narrow-spectrum-vs-broad-spectrum.html http://www.diffen.com/difference/Disinfect_vs_Sterilize B. List five sterilization methods, how they work, and what they are used for. The first form is steam. A machine called an autoclave is heated to 121-134 degrees Celsius. You hold the object there for 15 minutes for 121 degree Celsius or 3 minutes at 134 degree Celsius. It is used to inactivate all fungi, bacteria, viruses,...
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...Introduction Microorganisms are all around us and we interact with them all the time every day. Everyday microorganisms spread or grow. Contaminants such as bacteria and fungi are ubiquitous, meaning they exist everywhere, and they are able to spread or grow as well. Epidemiology is the study of how, when, where, what, and who are involved in the spread and distribution of diseases. The experiment presented was designed to study the epidemic of microorganisms in or on an object of our everyday life. The fish tank in the biology lab is a piece of equipment that houses an environment inside an environment, thus it changes constantly and deals with infinite microorganisms. My group of amateur scientists predicted that there is more bacteria living and spreading in the inside of the tank than the outside of the tank due to its environment it houses inside. The goal of the experiment is to prove using samples from cotton swabs inside and outside the tank and observing those 3 days in algar plates after how there is more colonies of bacteria inside than outside. Materials and Methods Materials we used to execute the experiment were a fish tank, 4 cottons swabs or q-tips, and 4 agar plates to contain the samples from the cotton swabs. The first step in the procedure was to find the naturally contaminated fish tank in our everyday environment. Next you acquire 4 cotton swabs or samples inside and outside the fish tank environment using q- tips. This is done by wiping 2 q- tips...
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... A. Describe the difference between an obligate and facultative aerobe. Obligate aerobe refers to the organisms that cannot survive in environments without any presence of oxygen. Facultative aerobes refer to the organisms that can survive in the presence of oxygen, but they do not require it to survive (Gillespie, Hawkey, & Wiley, 2006). B. Discuss the temperature requirements of the organisms in this lab. The temperature requirement of the organisms in this lab is approximately 37℃. These organisms require this optimum temperature for enzyme reactions to take place. Too low temperature below the optimum deactivates enzymes reactions while too high temperatures above optimum denature enzymes. S. cerevisiae, s. epidermis and l. acidophilus are facultative anaerobe (Gillespie, Hawkey, & Wiley, 2006). C. Discuss under what conditions you would want to control the growth of microbes. To grow bacteria in a laboratory the conditions in the lab needs to mimic the particular microbes natural environmental. To culture microbe’s sterile, disinfecting, aseptic techniques need to be used. For instance, aseptic techniques are also needed to transfer food to the microbe. Sterilization is a process of destroying all forms of life on an object or in a material including endospores. Disinfectant are chemicals which kill growing microbes, but not necessarily spore and are used on inanimate objects...
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...Cover page: Writing Assignments 1 & 2 William H Johnson Jr. Survey of the Sciences Week 1 Assignment 1 – Writing Assignment 1: Science in the media Assignment 2 – Writing Assignment 2: The Process of Science Date of assignment: 12/18/2013 Date turned in: 12/18/20 My best teacher was my French language instructor by the name of Cilote Yves, from France and he was the most encouraging and patient professor I have ever met. He always went the extra mile to make sure that his students understood what they were been taught and in turn have them implemented in real-time scenarios. He was both an instructor and a mentor and made his course very interesting and likeable. He provided all the tools and incentives that were needed for us to accomplish whatever project we undertook! He was also very involved in our lives on and off campus and was very, very, down-to-earth and did everything he could to make sure no one dropped out of school and stayed to the very end. He will truly remain one of the best to me! Cover page: Writing Assignments 1 & 2 William H Johnson Jr. Survey of the Sciences Week 1 Assignment 1 – Writing Assignment 1: Science in the media Date of assignment: 12/18/2013 Date turned in: 12/18/2013 Unit 1: writing Assignment 1. Science in the Media Title 1: Kids' Reading Success Boosted by Long-Term Individualized Instruction Results were based on studies and research carried out by scientists and psychologists, and teachers and...
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...Education and/or Training Required In order to work in this field you must have at least an undergraduate degree in microbiology. Further training with a Masters or Doctorate would be required to do anything other than entry level positions. Excellent microscopy skills and training in aseptic techniques are a must. * Responsibilities and Daily Activities This job requires a strong academic background with a lot of math, chemistry, and biology. Computer and lab skills are essential. Microbiologists grow cultures of bacteria (and other organisms) in medium for study. Using aseptic technique, they grow cultures to measure and calculate the rate of growth and how it grows. They learn what can and cannot kill their organisms and if it is a pathogen, they study ways to stop or slow its growth. In the lab, they work with microscopes, grow cultures, measure the rate of growth, and perform experiments with these organisms. Lab safety is very important in their work. They would NEVER eat or drink in the lab because this could cause contamination or could infect the food they are eating. * Salary Range Microbiologists can work in the academic community, industry or government. The following chart indicates the starting salary for these positions. Type of Work Place | Baccalaureate | Masters | Doctorate | Academic Institution | $21,000 | $29,000 | $46,000 | Industry | $21,000 | $35,000 | $66,000 | Government | $23,000 | $28,000 | $56,500 | * Documentation...
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...to identify the characterization, isolation and identification of microorganism from the desert soil. In the present study, thermal resistant and osmotolerant bacteria from the Thar soils were reported. Bacteria were isolated from 20 soil samples of 100gm each, collected from sand dunes. As Thar Desert were also evaluated for their plant growth promontory effects in terms of seed germination, shoot and root length elongation and total biomass production. It also tells about the occurrence of nodulated legumes. Most of the bacteria grow best in normal environmental conditions, but some grow in extreme conditions like extremes of pH, temperature, high salt reservoirs of microorganisms, as bacteria, protozoans, yeasts, molds, and microscopic worms are routinely found in this environment and are continuously submitted to environmental and anthropogenic perturbations which can lead to serious modifications of microbial taxonomic and functional diversity present in desert soil, therefore some of the fungi like Thermophilic fungi are also present in these conditions. Moreover DNA Based Identification and Characterization of microorganisms were also done from the soil of Thar Desert. Along with this, application of the microorganism isolated from the thar desert are like in biofertiliser, for medicinal parouses etc., example Ricinus communis etc., as nitrogen fixing organisms .Some of the examples of the microorganisms found in thar desert soil are rhizobacteria, Thermonospora archaea...
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...Title: Pond Microorganisms Lab Observation: Different types of organisms live in different ecosystems. Question (Problem): What types of microorganisms are found in pond water in a North Carolina pond? Hypothesis: If samples from a pond ecosystem are examined under a microscope, then ______________________________________________________________________ ______________________________________________________________________ Materials: (List the materials used in this experiment here.) Procedure: 1. Collect a few drops of water from the bottom of your water sample using an eye dropper. 2. Place a drop on the center of the microscope slide. 3. Cover the slide with a cover slip by lowering the cover slip at an angle over the water drop in a manner that spreads out the water drop, but does not trap air bubbles. 4. Observe the prepared slide under the microscope. 5. Use the picture ID sheet to identify the organisms observed. 6. Draw a picture in a data table of each organism observed. 7. Write the name of the organism under the picture, if the organism can be identified. 8. Describe each organism using qualitative (adjectives) and quantitative (measurements and counts) terms. 9. Repeat steps 1 to 8 until enough organisms are found. Results: (Describe what you observed. List the different types of organisms found and how many of each type of organism was found. Write a description of each organism (step 8).) Hint: A data table with a title and...
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...What is a Microbiologist? If you become a microbiologist, you may isolate organism cultures and classify the types of organisms found in soil, water and living hosts. You might also observe how organisms interact with each other and with living plant and animal tissue, as well as analyze the relationship between organisms and disease. Some of your duties could include preparing technical reports and research papers detailing your findings and making recommendations based on your research. You might also supervise lab workers and technicians and perform lab experiments for government health departments and physicians. A microbiologist is a scientist who studies the properties of fungi, algae, bacteria and other microscopic organisms. Areas of specialization include virology, immunology or bioinformatics. The following chart provides an overview about this career. Degree Required Bachelor's degree Field of Study Microbiology, biochemistry or cell biology BS Biology is the course needed to take Microbiology. Microbiology Training For over 80 years we have been training the food industry and we pride ourselves on the quality and content of our training portfolio. We offer a range of microbiology training to the food and drinks industry from standard courses on HACCP (RSPH), shelf-life and auditing to food microbiology and tailored confidential training. In partnership with Skill gate we have developed an online microbiology course that covers eight basic modules...
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...Introduction Morphological, staining, cultural and biochemical data on the characteristics of different microorganisms was gathered. This gained information can be used to identify bacteria. A scientist by the name of David Bergey was the first person who proposed the system of bacterial classification in which bacteria are grouped according to Gram reaction, metabolism, and morphology. The first edition of Bergey’s Manual of Systematic Bacteriology was published in 1984. This book can be used to identify a microorganism. The purpose of this experiment is to identify an unknown bacteria using the skills learned in microbiology laboratory this semester. Materials and Methods This experiment was conducted at Louisiana State University in Shreveport...
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...Yasmin Martinez Biology 2310 Lab Report I Antibiotics, Antiseptics, and Disinfectants June 22, 2018 Introduction As the world continues to evolve, bacteria evolves along with it. This can begin to impact the effect of antimicrobials, which are used to inhibit or kill the growth of microorganisms. Antimicrobial testing is important to determine the susceptibility within a given agent. It is also important to consider resistance of an antimicrobial agent to a given microorganism. Antimicrobial resistance occurs when a given drug loses its effect due to the microbes changing, either because of mutation or they acquire new genetic information from other microbes.1 Materials and Methods The materials required for this lab are the four bacterial broth cultures: Staphylococcus saprophyticus, Escherichia coli, Pseudomonas fluorescens, and Bacillus subtilis. Eight trypticase soy agar (TSA) plates, a Kirby-Bauer Antibiotic Disk Dispenser, a container with sterile filter paper disk, another container with 70% alcohol, forceps, sterile swabs, Hydrogen Peroxide, Bleach, Antiseptic Mouth Wash,...
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...Title: Pond Microorganisms Lab Observation: Different types of organisms live in different ecosystems. Question (Problem): What types of microorganisms are found in pond water in a North Carolina pond? Hypothesis: If samples from a pond ecosystem are examined under a microscope, then ______________________________________________________________________ ______________________________________________________________________ Materials: (List the materials used in this experiment here.) Procedure: 1. Collect a few drops of water from the bottom of your water sample using an eye dropper. 2. Place a drop on the center of the microscope slide. 3. Cover the slide with a cover slip by lowering the cover slip at an angle over the water drop in a manner that spreads out the water drop, but does not trap air bubbles. 4. Observe the prepared slide under the microscope. 5. Use the picture ID sheet to identify the organisms observed. 6. Draw a picture in a data table of each organism observed. 7. Write the name of the organism under the picture, if the organism can be identified. 8. Describe each organism using qualitative (adjectives) and quantitative (measurements and counts) terms. 9. Repeat steps 1 to 8 until enough organisms are found. Results: (Describe what you observed. List the different types of organisms found and how many of each type of organism was found. Write a description of each organism (step 8).) Hint: A data table with a title and...
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...Unknown Laboratory Report Sarah Mansfield Biology 203 Professor Lana November 19, 2014 INTRODUCTION Microorganisms are the earliest forms of life and most have thrived since the early birth of the planet. They have evolved into many different forms of life such as animals, humans, and plants. While some microorganisms impact life in a positive way by sustaining life, others cause disease and even death (Nester, et al. 2012). Prokaryotes are one of the earliest organisms to exist, and many of these organisms can thrive in conditions far worse than other living things. For example, they can tolerate extreme temperatures, acidic, or alkaline environments (Foster 1996). Prokaryotic cells do...
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...it required oxygen to grow. This test took me to the next test to check if my bacteria had Cytochrome Oxidase in its electron transport chain. If the test shows a positive, then the bacteria uses oxygen as electron acceptor. I got a wood stick and rubbed some of my bacteria onto the oxidase card. Within 20 seconds the oxidase card turned blue indicating a positive result. This also explains my result from the gas pack test. Oxygen is important to my bacteria. All known organisms can be lined up by their size. To figure out what size the bacteria I did a simple stain of my bacteria to figure out the size of it. Inspecting the slide under the microscope, it was revealed that my bacteria is less than 2um. Viruses are the smallest microorganism out there ranging from 20nm to 300nm. They are invisible to the naked eye. An electron microscope is needed to visualize them. Acid and base test helps to know which type of environment is beneficial to a bacteria. To figure out my family I had to inoculate a 3.6pH broth to see if my bacteria can survive acidic environment. I inoculated the broth and placed it into a 37C incubator for 48 hours. When I came back to inspect the broth, I found there was no growth. This test finally helped me to narrow down the bacteria family, which is Pseudomonas. Now that I knew I was working with the Pseudomonas family, there were quite of few tests I needed to complete to figure out which genus/species I had. I introduced 6 different sugars:...
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...Feeding Operations (CAFO). A CAFO functions as an industrial plant and its only purpose is to produce meat products as rapidly and as lucratively as they possibly can. A CAFO is an agricultural operation that congregates animals, feed, manure, urine, dead animals, and production operations on a small land area. The production methods used in these operations is the cause for measurable damages across a wide range of environmental, biological, and economic factors. (Institute of Science, Technology, and Public Policy, 2008) One of these factors is a public health one. The extensive use of antibiotics in livestock CAFO’s, especially for non-therapeutic uses such as growth promotion contributes to the development of antibiotic-resistant microorganisms that are more difficult to treat and are causing food-borne diseases in humans. It is important to identify and reduce the unnecessary use of antibiotics on these animals in order to prevent the creation of antibiotic-resistant bacteria. Some of the Difficulties For decades, antibiotics such as Tylosin and Monensin have been used for the treatment of diseases in animals and humans. The United States Food and Drug Administration approved in the 1950’s their use in animal feed as long as they are given “in low doses to help animals grow faster, produce more meat, and avoid illness.” (Food Market Institute, n.d., p. 1) According to Tam & Willson, (2011) “antibiotics are also routinely placed in livestock feed and water to increase...
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