...|Point Spread |Point Spread |Point Spread |Point Spread |Point Spread | |Grammar, punctuation, |10% |Student has 0-2 mechanical errors and no spelling|Student has 2-4 mechanical and/or|Student has 4-6 mechanical and/or |Student has 6-8 mechanical and/or |Same as D | |spelling, mechanics | |errors. No run-on sentences or fragments. |spelling errors |spelling errors, such as a run-on |spelling errors. Appears to lack |0-5 | | | |Student does not write in the second person. |8 |or fragment |proofreading. Run on sentences and| | |= 10% | |9 | |7 |fragments | | | | | | | |6 | | |Citations/MLA |10% |Proper MLA usage and citations, extent...
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...I am going to tell you why I should pass this English 3000.01 class for that I have learned a lot in writing essays. I have learned to use subordination and coordinating conjunctions in my essays when I never thought of using them before and I have become more proficient in preventing fragment and run-ons sentences and I have learned to write a great complete argumentative essay. I have learned to use subordination and coordinating conjunctions in my essays when I never thought of using them before. I have learned to use subordinating conjunctions that introduces a dependent clause by joining it to a main clause. Words like because, when, if and more that help build a sentence better. I even learned that coordinating conjunctions are joining two similarly constructed words or phrases within a sentence. Words like for, or, yet, and more that help in a sentence. I have uses both coordinating and subordination conjunctions in my papers. One sentence that I used a subordinating conjunction “People need to understand that there are people out in our world who do not care about their privacy, when they post everything online” (Gallego) and I used a coordinating conjunction in this paragraph “Think about the idea that everyone achieved a college degree, for all people would be equal in...
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...Fragments, Run-ons and Comma Splices Getting Rid of Fragments There are two kinds of clauses – independent and dependent. A dependent clause has a subject and verb just like an independent clause, but it can’t stand alone because it begins with a dependent word such as after that, so that although, though unless as, as if until because what, whatever before when, whenever even though where, wherever how whether if, even if which, whichever in order that while since who, whom than whose Whenever a clause begins with one of the above dependent words, it is dependent. If we take an independent clause such as We finished the game. and put one of the dependent words in front of it, it becomes dependent: After we finished the game Although we finished the game As we finished the game Before we finished the game If we finished the game Since we finished the game That we finished the game When we finished the game While we finished the game The clause can no longer stand alone. It does not make a complete statement. It leaves the reader expecting something more. It is a fragment and must not be punctuated as a sentence. Any group of words that does not have a subject and a verb is also a fragment. Paid no attention to his parents (no subject) Joe thinking of all his problems (no adequate verb) Speeding along the highway (no subject and no adequate verb) The announcement...
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...field. The components needed in order to run electrophoresis are cathodes( positive, negative), medium for the sample, and the electric current most importantly. The electric current is responsible for creating the field of electricity, and networking the sample that is placed inside. Agarose was the liquid used in order to conclude the size of the DNA fragments. Agarose contains pores which allows DNA to go through, and separate into different size fragments. Gel electrophoresis is known as the movement separation of fragments of DNA due to factors such as size, and charge. In order to be able to identify the size of DNA a ladder needs to be present in order to determine the size in base pairs. DNA moves from negative to positive. DNA is negative due to phosphate group on the backbone, hence it will move towards the positive end. Smaller fragments that have a lower density will move further than the larger fragments with a higher density. The goal of the overall project is to demonstrate how gel electrophoresis works. The theory behind gel electrophoresis allows one to analyze DNA by the separation of size which is a great thing in the world of biochemistry. Thanks to this method of gel...
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...Gel electrophoresis Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.[1] Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through an agarose matrix. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.[2] Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles. Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied.[3] DNA Gel electrophoresis is usually performed for analytical purposes, often after...
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...structure which consists of fragments, run-ons, commas, and final punctuations. The steps that were mastered were the Craft of writing step. I at first did not understand all of the steps and what they consist of; I later begin to understand them. How do the skills apply to your academic life? Academically the steps are going to be very valuable because in order to write a correct paper in college I must first master these steps. By me not knowing how to identify fragments can put a huge dent in my grades on essays and other assignments. Punctuation is an important part of just writing in general. Punctuation to me expresses the tone of a sentence and or essay. I don’t want to have run on sentences. I would like to be able to identify run on sentences. How do the skills apply to your professional life? As a professional person people are going to rely on you to write and or present presentations. If you have run on sentences, fragments, and you aren’t using puncation correctly they are going to start to wonder what type of person they have working for them . It is important to be very professional in a professional environment. You not only have to look at certain way you also have to speak a certain ways and write a certain way. Were you surprised by the result? Why or why not? I actually was not surprised by the results just for the simple fact of me not being a very good writer in high school. I have always had a hard time identifying fragments especially. I agree totally...
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...error I continuously made. The essays I have written thus far I have used these word incorrectly twelve times. To correct these errors, I should first know what each word means. There, is at or in a place. There is also used in clauses which the real subject follows the verb for example; Burning calories isn’t easy but there are soothing exercises available. The definition for “their” is belonging to, made by or done by them. The boys went with their father is an example of their used correctly. Other errors I have made during essay writing are fragments and run ons. A fragment is an incomplete part. Michael Jackson began performing at age thirteen is an example of a fragment. To correct this example I need a subject and verb; therefore, Michael Jackson began performing with the Jackson Five at age thirteen is a complete sentence. Run on sentences occur when you do not properly punctuate sentences. An example of a run sentence is: Although all venereal diseases are unsafe the newest member AIDS is extremely dangerous and deadly there is...
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...CHAPTER 7 3. Write the query that will generate a combined list of customers (from tables CUSTOMER and CUSTOMER_2) that do not include the duplicate customer records. (Note that only the customer named Juan Ortega shows up in both customer tables.) SELECT CUST_LNAME, CUST_FNAME FROM CUSTOMER UNION SELECT CUST_LNAME, CUST_FNAME FROM CUSTOMER_2; 4. Write the query that will generate a combined list of customers to include the duplicate customer records. SELECT CUST_LNAME, CUST_FNAME FROM CUSTOMER UNION ALL SELECT CUST_LNAME, CUST_FNAME FROM CUSTOMER_2; 5. Write the query that will show only the duplicate customer records. We have shown both Oracle and MS Access query formats: Oracle SELECT CUST_LNAME, CUST_FNAME FROM CUSTOMER INTERSECT SELECT CUST_LNAME, CUST_FNAME FROM CUSTOMER_2; MS Access SELECT C.CUST_LNAME, C.CUST_FNAME FROM CUSTOMER AS C, CUSTOMER_2 AS C2 WHERE C.CUST_LNAME=C2.CUST_LNAME AND C.CUST_FNAME=C2.CUST_FNAME; Because Access doesn’t support the INTERSECT SQL operator, you need to list only the rows in which all the attributes match. 6. Write the query that will generate only the records that are unique to the CUSTOMER_2 table. We have shown both Oracle and MS Access query formats: Oracle SELECT CUST_LNAME, CUST_FNAME FROM CUSTOMER_2 MINUS SELECT CUST_LNAME, CUST_FNAME FROM CUSTOMER; MS Access SELECT C2.CUST_LNAME...
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...Agarose Gel Electrophoresis for the Separation of DNA Fragments Pei Yun Lee, John Costumbrado, Chih-Yuan Hsu, Yong Hoon Kim Department of Molecular, Cell, and Developmental Biology, University of California Los Angeles Video Article Chapters 0:05 Title 1:31 Preparation of the Gel 3:21 Setting up Gel Apparatus and Separating DNA Fragments 4:55 Observing Separated DNA Fragments 5:43 Results: Agarose Gel Electrophoresis of PCR Products 6:23 Conclusion Cite this Article Lee, P. Y., Costumbrado, J., Hsu, C. Y., Kim, Y. H. Agarose Gel Electrophoresis for the Separation of DNA Fragments. J. Vis. Exp. (62), e3923, doi:10.3791/3923 (2012). Abstract Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb1. Agarose is isolated from the seaweed genera Gelidium and Gracilaria, and consists of repeated agarobiose (L- and D-galactose) subunits2. During gelation, agarose polymers associate non-covalently and form a network of bundles whose pore sizes determine a gel's molecular sieving properties. The use of agarose gel electrophoresis revolutionized the separation of DNA. Prior to the adoption of agarose gels, DNA was primarily separated using sucrose density gradient centrifugation, which only provided an approximation of size. To separate DNA using agarose gel electrophoresis, the DNA is loaded into pre-cast wells in the gel and a current applied. The phosphate backbone of the DNA (and RNA) molecule...
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...Study Session at Alicia’s Transition: General Description: Alicia is finishing cooking while hosting a study session with our Student, Gabby and Quentin. She’s a bit nervous, as the in-laws are coming over that evening (on short notice). They talk about the challenges of writing for college courses. Dialogue: Alicia I’m so glad you all came over. Thanks for letting me cook during our study session. My in-laws told us at the last minute that they’re coming over later this evening, and I can really use your moral support! Gabby No problem, Alicia. Everything smells wonderful; I’m sure your in-laws’ visit will go just fine. Quentin Yeah, and I really needed this study session, too. I’m a little anxious about my current writing assignment. Maybe I can create a computer program so that my paper writes itself! I could really use some help like that. Student Text Bubble Writing for college courses sure is challenging. Gabby It’s challenging, but the reward is well worth the effort. Quentin, you should take your assignment seriously. I’ve found that, in my classes as well as in my marketing and communications field, the quality of your writing really matters. Alicia Gabby’s right. Lots of people fear the writing process, but as long as you’re enthusiastic and positive about it, you’ll be successful. And Quentin, if you need help, just use the CWE. Student Text Bubble What is the CWE? Alicia It’s the University’s Center for...
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...Android Tutorial ANDROID TUTORIAL Simply Easy Learning by tutorialspoint.com tutorialspoint.com i ABOUT THE TUTORIAL Android Tutorial Android is an open source and Linux-based operating system for mobile devices such as smartphones and tablet computers. Android was developed by the Open Handset Alliance, led by Google, and other companies. This tutorial will teach you basic Android programming and will also take you through some advance concepts related to Android application development. Audience This tutorial has been prepared for the beginners to help them understand basic Android programming. After completing this tutorial you will find yourself at a moderate level of expertise in Android programming from where you can take yourself to next levels. Prerequisites Android programming is based on Java programming language so if you have basic understanding on Java programming then it will be a fun to learn Android application development. Copyright & Disclaimer Notice All the content and graphics on this tutorial are the property of tutorialspoint.com. Any content from tutorialspoint.com or this tutorial may not be redistributed or reproduced in any way, shape, or form without the written permission of tutorialspoint.com. Failure to do so is a violation of copyright laws. This tutorial may contain inaccuracies or errors and tutorialspoint provides no guarantee regarding the accuracy of the site or its contents including this tutorial. If you discover...
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...Some Types of Grammars I. Phrases Phrase is a group of related words that does not include a subject and verb. There are different kind of phrases: -Noun Phrases: A noun phrase consists of a noun and all its modifiers. Example: -The lost puppy was a wet and stinky dog. -It was a story as old as time. -Verb Phrases: A verb phrase consists of a verb and all its modifiers. Example: -He was waiting for the rain to stop. -You have been sleeping for a long time. -Gerund Phrases: A gerund phrase is simply a noun phrase that starts with a gerund. Example: -Taking my dog for a walk is fun. -Walking in the rain can be difficult. -Infinitive Phrases: An infinitive phrase is a noun phrase that begins with an infinitive. Example: -Everybody loves to watch movies. -I went to Spain to study the language and culture. -Appositive Phrases: An appositive phrase restates a noun and consists of one or more words. Example: -My wife, the love of my life, is also my best friend. -My idea, to recycle the paper, was accepted by the boss. -Participial Phrases: A participial phrase begins with a past or present participle. Example: -I am really excited, considering all the people that will be there. -We are looking forward to the movie, having seen the trailer last week. -Prepositional Phrases: A prepositional phrase begins with a preposition and can act as a noun, an adjective or an adverb. Example: -The book was on the table. -She was lost in...
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...LATEST BIOCHEMICAL TECHNIQUES CAPILLARY ELECTROPHORESIS Capillary electrophoresis is an analytical technique that separates ions based on their electrophoretic mobility with the use of an applied voltage. The electrophoretic mobility is dependent upon the charge of the molecule, the viscosity, and the atom’s radius. In conventional electrophoresis, electrically charged analytes move in a conductive liquid medium under the influence of an electric field. The rate at which the particles moves is directly proportional to the applied electric field; the greater the field strength, the faster the mobility. If two ions are the same size, the greater charge will move the fastest. For ions of the same charge, the smaller particle has less friction and overall faster migration rate. The technique of capillary electrophoresis was designed to separate species based on their size to charge ratio in the interior of a small capillary filled with an electrolyte. Capillary electrophoresis is used most predominately because it gives faster results and provides high resolution separation. It is a useful technique because there is a large range of detection methods available. PRINCIPLE Electrophoresis is the process whereby the movement of ions is produced under the influence of an applied voltage across a field that the ions exist. In electrophoresis, ions that are negatively charged will move or migrate towards the positively charged electrode while ions that are positively charged will...
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...ENG-105 Rubric: Rhetorical Analysis of a Public Document Assignment |Criteria |% Value |1: Unsatisfactory |2: Less Than Satisfactory |3: Satisfactory |4: Good |5: Excellent | |% Scaling | |0% |65% |75% |85% |100% | |Content and Ideas – 60% | |Introduce and summarize the |20% |The introduction of the website is|The introduction of the website|The introduction of the website is |The introduction of the |The introduction to the website| |website | |not present AND the summarization |is not present OR the |present. The summarization of the |website is present. The |is present and elaborate. The | | | |of the website is missing. |summarization of the website is|website is present. |summarization of the website |summarization of the website is| | | | |incomplete. | ...
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...obtained from genomic analysis (via PCR and gel electrophoresis) confirmed that the genotypic result is consistent with the phenotypic result observed at the beginning of the lab. However, DNA fragments of 3 lab subjects didn’t show up on the gel. The allele frequencies can’t be calculated because the data is insufficient to apply the Hardy-Weinberg equation. There are many factors that might be contributed to the invisibility of these DNA fragments, most likely accidental errors. For example, the DNA wasn’t loaded onto the gel probably, or the DNA for some reason didn’t sink to the bottom of the well, or just simply there was not enough DNA. To determine the genotypic profile of the students PTC gene, DNA samplers from each individual was collected from saliva. Using premade PTC primers (short oligonucleotides), a DNA template that encoded the PTC gene (approximately, 303 bp) was amplified by PCR. After amplification, the produced DNA fragments were digested with Fnu4H1 to identify if the lab subjects have a C or a T at position 785 (PTC taster gene location, Figure 3.) Fnu4H1 (5’ GCTGC 3’) is an enzyme that cuts DNA at specific restriction length polymorphism (RFLP) site present only in wild type alleles and absent in mutant alleles ((5’ GTTGC 3’.) Digested DNA will be cut into 2 fragments 239 bp and 64 bp in length. (Figure 2) This discrepancy in length allowed us to profile the alleles...
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